HIK34_SYNY3
ID HIK34_SYNY3 Reviewed; 464 AA.
AC P74199;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Sensor histidine kinase Hik34 {ECO:0000303|PubMed:15471853};
DE EC=2.7.13.3 {ECO:0000269|PubMed:15965020};
GN Name=hik34 {ECO:0000303|PubMed:15471853};
GN OrderedLocusNames=slr1285 {ECO:0000312|EMBL:BAA18290.1};
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
RN [2]
RP FUNCTION IN HYPEROSMOTIC STRESS RESPONSE, REGULON, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=15471853; DOI=10.1074/jbc.m410162200;
RA Paithoonrangsarid K., Shoumskaya M.A., Kanesaki Y., Satoh S., Tabata S.,
RA Los D.A., Zinchenko V.V., Hayashi H., Tanticharoen M., Suzuki I.,
RA Murata N.;
RT "Five histidine kinases perceive osmotic stress and regulate distinct sets
RT of genes in Synechocystis.";
RL J. Biol. Chem. 279:53078-53086(2004).
RN [3]
RP FUNCTION IN SALT STRESS RESPONSE, REGULON, AND DISRUPTION PHENOTYPE.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=15805106; DOI=10.1074/jbc.m412174200;
RA Shoumskaya M.A., Paithoonrangsarid K., Kanesaki Y., Los D.A.,
RA Zinchenko V.V., Tanticharoen M., Suzuki I., Murata N.;
RT "Identical Hik-Rre systems are involved in perception and transduction of
RT salt signals and hyperosmotic signals but regulate the expression of
RT individual genes to different extents in synechocystis.";
RL J. Biol. Chem. 280:21531-21538(2005).
RN [4]
RP FUNCTION IN HEAT SHOCK RESPONSE, REGULON, CATALYTIC ACTIVITY, INDUCTION,
RP AUTOPHOSPHORYLATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=15965020; DOI=10.1104/pp.104.059097;
RA Suzuki I., Kanesaki Y., Hayashi H., Hall J.J., Simon W.J., Slabas A.R.,
RA Murata N.;
RT "The histidine kinase Hik34 is involved in thermotolerance by regulating
RT the expression of heat shock genes in synechocystis.";
RL Plant Physiol. 138:1409-1421(2005).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=19411329; DOI=10.1128/jb.00183-09;
RA Vidal R., Lopez-Maury L., Guerrero M.G., Florencio F.J.;
RT "Characterization of an alcohol dehydrogenase from the Cyanobacterium
RT Synechocystis sp. strain PCC 6803 that responds to environmental stress
RT conditions via the Hik34-Rre1 two-component system.";
RL J. Bacteriol. 191:4383-4391(2009).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=25738257; DOI=10.3390/life5010676;
RA Cerveny J., Sinetova M.A., Zavrel T., Los D.A.;
RT "Mechanisms of High Temperature Resistance of Synechocystis sp. PCC 6803:
RT An Impact of Histidine Kinase 34.";
RL Life 5:676-699(2015).
CC -!- FUNCTION: Member of a two-component system Hik34/Rre1, controlling
CC expression of at least 20 genes in response to hyperosmotic stress (0.5
CC M sorbitol) or salt (0.5 M NaCl) (PubMed:15471853, PubMed:15805106,
CC PubMed:19411329). Represses expression of heat shock genes under normal
CC growth conditions (PubMed:15965020). Required for survival of long-term
CC heat shock exposure (PubMed:25738257). {ECO:0000269|PubMed:15471853,
CC ECO:0000269|PubMed:15805106, ECO:0000269|PubMed:15965020,
CC ECO:0000269|PubMed:19411329, ECO:0000269|PubMed:25738257}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3; Evidence={ECO:0000269|PubMed:15965020};
CC -!- INDUCTION: Expression strongly induced by heat shock at 44 degrees
CC Celsius for 20 minutes, after 60 minutes expression has fallen.
CC {ECO:0000269|PubMed:15965020}.
CC -!- PTM: When expressed in E.coli autophosphorylates at 18 to 30 degrees
CC Celsius; less phosphorylation occurs at 36 and none occurs at 42 or 48
CC degrees Celsius. {ECO:0000269|PubMed:15965020}.
CC -!- DISRUPTION PHENOTYPE: Loss or reduction of expression of about 20 genes
CC in response to hyperosmotic stress (0.5 M sorbitol) or salt (0.5 M
CC NaCl) stress (PubMed:15471853, PubMed:15805106, PubMed:19411329).
CC Increased survival after heat shock from 30 to 48 degrees Celsius for
CC 2-3 hours. Increased expression of some heat shock and photosystem-
CC related proteins (PubMed:15965020). Cells die after 24 hours of heat
CC shock (32 to 44 degrees Celsius). Increased expression of some heat
CC shock proteins during normal growth (PubMed:25738257).
CC {ECO:0000269|PubMed:15471853, ECO:0000269|PubMed:15805106,
CC ECO:0000269|PubMed:15965020, ECO:0000269|PubMed:19411329,
CC ECO:0000269|PubMed:25738257}.
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DR EMBL; BA000022; BAA18290.1; -; Genomic_DNA.
DR PIR; S75831; S75831.
DR AlphaFoldDB; P74199; -.
DR SMR; P74199; -.
DR IntAct; P74199; 5.
DR STRING; 1148.1653376; -.
DR PaxDb; P74199; -.
DR EnsemblBacteria; BAA18290; BAA18290; BAA18290.
DR KEGG; syn:slr1285; -.
DR eggNOG; COG0642; Bacteria.
DR InParanoid; P74199; -.
DR OMA; RMELIFR; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR CDD; cd00082; HisKA; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR Pfam; PF00512; HisKA; 1.
DR SMART; SM00388; HisKA; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
PE 1: Evidence at protein level;
KW Kinase; Phosphoprotein; Reference proteome; Stress response; Transferase;
KW Two-component regulatory system.
FT CHAIN 1..464
FT /note="Sensor histidine kinase Hik34"
FT /id="PRO_0000453142"
FT DOMAIN 235..449
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 238
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ SEQUENCE 464 AA; 52623 MW; A3D94EB293D7B3DF CRC64;
MNEVCLKLSD LFVSSGWGGY DRGRAPQWAH PRAQQQWFGA IAALEPFLRQ TLPNVGGELP
GICLTGPAPV LKDAVLVRNF YQGIATPWEE FSPWPCLAGE ESEWSAVPPM REIPLFPQDP
LAEEQFCWLM TPQFGLLLLL GKNEQGLAQF YWTFDPEILQ QAWLSLQARL KYGLSPDLSL
LQKTIAAFNF PQPDFRLVTY FGQLMLDYQP NPYNLPPCQE QESAEPSPDV ELLQALTHEV
RTPLTSIRTL TKLLLRRKDL SPEVLKRIES IDRECSDQIS RMDLIFRATE LESTPLPELV
VPLTVTSLEA VFQAGIPRWQ KQAQRYNVNL QAQIPHSLPQ VWSNPSLLDQ VLGGMIEKFV
RNFNGGGEIN LQITTAGDQL KVQFHTQSVH QANPVRALGE LLMFQPQTGC LSLNWDVTKN
LFQLLGGKLI VRRRSPSEEI LTIYLKCEQR TVPVANYDRQ FTMV
