ID   HIKES_BOVIN             Reviewed;         197 AA.
AC   Q56JY0;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Protein Hikeshi {ECO:0000250|UniProtKB:Q53FT3};
GN   Name=HIKESHI {ECO:0000250|UniProtKB:Q53FT3};
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lymphoid epithelium;
RA   Yu J., Meng Y., Wang Z., Hansen C., Li C., Moore S.S.;
RT   "Analysis of sequences obtained from constructed full-length bovine cDNA
RT   libraries.";
RL   Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Heart ventricle;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts as a specific nuclear import carrier for HSP70 proteins
CC       following heat-shock stress: acts by mediating the nucleoporin-
CC       dependent translocation of ATP-bound HSP70 proteins into the nucleus.
CC       HSP70 proteins import is required to protect cells from heat shock
CC       damages. Does not translocate ADP-bound HSP70 proteins into the nucleus
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms an asymmetric homodimer; required for binding and
CC       nuclear import of HSP70 proteins. Interacts with ATP-bound HSP70
CC       proteins. Interacts with NUP62 and NUP153 (via F-X-F-G repeats).
CC       Interacts with HSPA8. {ECO:0000250|UniProtKB:Q53FT3}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9DD02}.
CC       Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q53FT3}. Nucleus
CC       {ECO:0000250|UniProtKB:Q53FT3}.
CC   -!- SIMILARITY: Belongs to the OPI10 family. {ECO:0000305}.
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DR   EMBL; AY911348; AAW82115.1; -; mRNA.
DR   EMBL; BC103227; AAI03228.1; -; mRNA.
DR   RefSeq; NP_001029398.1; NM_001034226.2.
DR   AlphaFoldDB; Q56JY0; -.
DR   SMR; Q56JY0; -.
DR   STRING; 9913.ENSBTAP00000026632; -.
DR   PaxDb; Q56JY0; -.
DR   PeptideAtlas; Q56JY0; -.
DR   PRIDE; Q56JY0; -.
DR   Ensembl; ENSBTAT00000026632; ENSBTAP00000026632; ENSBTAG00000019995.
DR   GeneID; 504867; -.
DR   KEGG; bta:504867; -.
DR   CTD; 51501; -.
DR   VEuPathDB; HostDB:ENSBTAG00000019995; -.
DR   VGNC; VGNC:29853; HIKESHI.
DR   eggNOG; KOG4067; Eukaryota.
DR   GeneTree; ENSGT00390000004056; -.
DR   HOGENOM; CLU_084839_2_0_1; -.
DR   InParanoid; Q56JY0; -.
DR   OMA; RMEQNPN; -.
DR   OrthoDB; 1223488at2759; -.
DR   TreeFam; TF313222; -.
DR   Reactome; R-BTA-3371453; Regulation of HSF1-mediated heat shock response.
DR   Proteomes; UP000009136; Chromosome 29.
DR   Bgee; ENSBTAG00000019995; Expressed in semimembranosus muscle and 108 other tissues.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0016607; C:nuclear speck; IEA:Ensembl.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0030544; F:Hsp70 protein binding; ISS:UniProtKB.
DR   GO; GO:0061608; F:nuclear import signal receptor activity; IBA:GO_Central.
DR   GO; GO:0034605; P:cellular response to heat; ISS:UniProtKB.
DR   GO; GO:0007030; P:Golgi organization; IEA:Ensembl.
DR   GO; GO:0030324; P:lung development; IEA:Ensembl.
DR   GO; GO:0006606; P:protein import into nucleus; ISS:UniProtKB.
DR   GO; GO:0015031; P:protein transport; ISS:UniProtKB.
DR   InterPro; IPR008493; DUF775.
DR   InterPro; IPR031318; OPI10.
DR   PANTHER; PTHR12925; PTHR12925; 1.
DR   Pfam; PF05603; DUF775; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nucleus; Protein transport; Reference proteome; Transport.
FT   CHAIN           1..197
FT                   /note="Protein Hikeshi"
FT                   /id="PRO_0000245261"
FT   REGION          18..55
FT                   /note="Required for F-X-F-G repeats-nucleoporins
FT                   recognition and nuclear import"
FT                   /evidence="ECO:0000250|UniProtKB:Q53FT3"
FT   REGION          124..134
FT                   /note="Flexible linker region involved in nuclear import of
FT                   HSP70 proteins"
FT                   /evidence="ECO:0000250|UniProtKB:Q53FT3"
SQ   SEQUENCE   197 AA;  21653 MW;  B63129391C5D4655 CRC64;
     MFGCLVAGRL VQTAAQQVAE DKFVFDLPDY ENINHVVVFM LGTVPFPEGM GGSVYFSYPD
     SNGMPVWQLL GFVTNGKPSA IFKISGLKSG EGSQHPFGTM NIVRTPSVAQ IGISVELLDS
     LAQQTPVGNA AVSSVDSFTQ FTQKMLDNFY NFASSFAVSQ AQMTPSPSEM FIPANVVLKW
     YENFQRRLAQ NPLFWKT