HIKES_RAT
ID HIKES_RAT Reviewed; 197 AA.
AC Q5M808; G3V889;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 2.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Protein Hikeshi {ECO:0000305};
GN Name=Hikeshi {ECO:0000312|RGD:1359455};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Spleen;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Acts as a specific nuclear import carrier for HSP70 proteins
CC following heat-shock stress: acts by mediating the nucleoporin-
CC dependent translocation of ATP-bound HSP70 proteins into the nucleus.
CC HSP70 proteins import is required to protect cells from heat shock
CC damages. Does not translocate ADP-bound HSP70 proteins into the nucleus
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Forms an asymmetric homodimer; required for binding and
CC nuclear import of HSP70 proteins. Interacts with ATP-bound HSP70
CC proteins. Interacts with NUP62 and NUP153 (via F-X-F-G repeats).
CC Interacts with HSPA8. {ECO:0000250|UniProtKB:Q53FT3}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9DD02}.
CC Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q53FT3}. Nucleus
CC {ECO:0000250|UniProtKB:Q53FT3}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5M808-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5M808-2; Sequence=VSP_043956;
CC -!- SIMILARITY: Belongs to the OPI10 family. {ECO:0000305}.
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DR EMBL; CH473956; EDM18567.1; -; Genomic_DNA.
DR EMBL; BC088340; AAH88340.1; -; mRNA.
DR RefSeq; NP_001013919.1; NM_001013897.1. [Q5M808-2]
DR RefSeq; XP_006229724.1; XM_006229662.3. [Q5M808-1]
DR AlphaFoldDB; Q5M808; -.
DR SMR; Q5M808; -.
DR STRING; 10116.ENSRNOP00000023468; -.
DR PaxDb; Q5M808; -.
DR PeptideAtlas; Q5M808; -.
DR PRIDE; Q5M808; -.
DR Ensembl; ENSRNOT00000023468; ENSRNOP00000023468; ENSRNOG00000017383. [Q5M808-1]
DR GeneID; 293103; -.
DR KEGG; rno:293103; -.
DR UCSC; RGD:1359455; rat. [Q5M808-1]
DR CTD; 51501; -.
DR RGD; 1359455; Hikeshi.
DR eggNOG; KOG4067; Eukaryota.
DR GeneTree; ENSGT00390000004056; -.
DR HOGENOM; CLU_084839_2_0_1; -.
DR InParanoid; Q5M808; -.
DR OrthoDB; 1223488at2759; -.
DR TreeFam; TF313222; -.
DR Reactome; R-RNO-3371453; Regulation of HSF1-mediated heat shock response.
DR PRO; PR:Q5M808; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Proteomes; UP000234681; Chromosome 1.
DR Bgee; ENSRNOG00000017383; Expressed in quadriceps femoris and 20 other tissues.
DR ExpressionAtlas; Q5M808; baseline and differential.
DR Genevisible; Q5M808; RN.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0016607; C:nuclear speck; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0030544; F:Hsp70 protein binding; ISS:UniProtKB.
DR GO; GO:0061608; F:nuclear import signal receptor activity; ISO:RGD.
DR GO; GO:0034605; P:cellular response to heat; ISS:UniProtKB.
DR GO; GO:0007030; P:Golgi organization; ISO:RGD.
DR GO; GO:0030324; P:lung development; ISO:RGD.
DR GO; GO:0006606; P:protein import into nucleus; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; ISS:UniProtKB.
DR InterPro; IPR008493; DUF775.
DR InterPro; IPR031318; OPI10.
DR PANTHER; PTHR12925; PTHR12925; 1.
DR Pfam; PF05603; DUF775; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Nucleus; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..197
FT /note="Protein Hikeshi"
FT /id="PRO_0000245264"
FT REGION 18..55
FT /note="Required for F-X-F-G repeats-nucleoporins
FT recognition and nuclear import"
FT /evidence="ECO:0000250|UniProtKB:Q53FT3"
FT REGION 124..134
FT /note="Flexible linker region involved in nuclear import of
FT HSP70 proteins"
FT /evidence="ECO:0000250|UniProtKB:Q53FT3"
FT VAR_SEQ 1..39
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_043956"
SQ SEQUENCE 197 AA; 21619 MW; 69C2CF45B6EC8A2B CRC64;
MFGCLVAGRL VQTAAQQVAE DKFVFDLPDY ENINHVVVFM LGTIPFPEGM GGSVYFSYPD
SNGVPVWQLL GFVTNGKPSA IFKISGLKSG EGSQHPFGAM NIVRTPSVAQ IGISVELLDS
LAQQTPVGSA AVSSVDSFTQ FTQKMLDNFY NFASSFALSQ AQMTPNPSEM FIPANVVLKW
YENFQRRLAQ NPLFWKT
