HILA_SALTY
ID HILA_SALTY Reviewed; 553 AA.
AC P43015;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Transcriptional regulator HilA;
DE Short=Protein IagA;
GN Name=hilA; Synonyms=iagA; OrderedLocusNames=STM2876;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=SL1344;
RX PubMed=8817493; DOI=10.1111/j.1365-2958.1995.mmi_18040715.x;
RA Bajaj V., Hwang C., Lee C.A.;
RT "hilA is a novel ompR/toxR family member that activates the expression of
RT Salmonella typhimurium invasion genes.";
RL Mol. Microbiol. 18:715-727(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP FUNCTION.
RC STRAIN=SL1344;
RX PubMed=8951817; DOI=10.1046/j.1365-2958.1996.d01-1718.x;
RA Bajaj V., Lucas R.L., Hwang C., Lee C.A.;
RT "Co-ordinate regulation of Salmonella typhimurium invasion genes by
RT environmental and regulatory factors is mediated by control of hilA
RT expression.";
RL Mol. Microbiol. 22:703-714(1996).
RN [4]
RP FUNCTION.
RC STRAIN=SL1344, and SL5283;
RX PubMed=10931326; DOI=10.1046/j.1365-2958.2000.01991.x;
RA Lostroh C.P., Bajaj V., Lee C.A.;
RT "The cis requirements for transcriptional activation by HilA, a virulence
RT determinant encoded on SPI-1.";
RL Mol. Microbiol. 37:300-315(2000).
RN [5]
RP REGULATION OF PRG OPERON.
RC STRAIN=SL1344, and SL5283;
RX PubMed=11466291; DOI=10.1128/jb.183.16.4876-4885.2001;
RA Lostroh C.P., Lee C.A.;
RT "The HilA box and sequences outside it determine the magnitude of HilA-
RT dependent activation of P(prgH) from Salmonella pathogenicity island 1.";
RL J. Bacteriol. 183:4876-4885(2001).
RN [6]
RP REVIEW.
RX PubMed=11755416; DOI=10.1016/s1286-4579(01)01488-5;
RA Lostroh C.P., Lee C.A.;
RT "The Salmonella pathogenicity island-1 type III secretion system.";
RL Microbes Infect. 3:1281-1291(2001).
RN [7]
RP REGULATION BY HILC AND HILD.
RC STRAIN=SL1344;
RX PubMed=11442828; DOI=10.1046/j.1365-2958.2001.02462.x;
RA Schechter L.M., Lee C.A.;
RT "AraC/XylS family members, HilC and HilD, directly bind and derepress the
RT Salmonella typhimurium hilA promoter.";
RL Mol. Microbiol. 40:1289-1299(2001).
CC -!- FUNCTION: The main transcriptional regulator of the Salmonella
CC pathogenicity island 1 (SPI1) gene expression. Activates the expression
CC of invasion genes by a direct action at their promoters and also
CC indirectly by increasing the level of InvF. Also binds upstream of prgH
CC and directly activates the expression of prgHIJK operon.
CC {ECO:0000269|PubMed:10931326, ECO:0000269|PubMed:8817493,
CC ECO:0000269|PubMed:8951817}.
CC -!- INDUCTION: Expressed in response to both environmental conditions and
CC genetic regulatory factors. Transcription is subject to complex control
CC and is stimulated by the SPI1-encoded HilC and HilD.
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DR EMBL; U25352; AAD12579.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21756.1; -; Genomic_DNA.
DR PIR; S70817; S70817.
DR RefSeq; NP_461797.1; NC_003197.2.
DR RefSeq; WP_001120085.1; NC_003197.2.
DR AlphaFoldDB; P43015; -.
DR SMR; P43015; -.
DR STRING; 99287.STM2876; -.
DR PaxDb; P43015; -.
DR EnsemblBacteria; AAL21756; AAL21756; STM2876.
DR GeneID; 1254399; -.
DR KEGG; stm:STM2876; -.
DR PATRIC; fig|99287.12.peg.3032; -.
DR HOGENOM; CLU_034088_0_0_6; -.
DR OMA; FASAYGM; -.
DR PhylomeDB; P43015; -.
DR BioCyc; SENT99287:STM2876-MON; -.
DR PHI-base; PHI:563; -.
DR PHI-base; PHI:8302; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50005; TPR; 1.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Phosphoprotein; Reference proteome; TPR repeat;
KW Transcription; Transcription regulation; Two-component regulatory system;
KW Virulence.
FT CHAIN 1..553
FT /note="Transcriptional regulator HilA"
FT /id="PRO_0000081108"
FT REPEAT 372..405
FT /note="TPR"
FT DNA_BIND 11..107
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 62
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000250"
FT CONFLICT 28
FT /note="L -> V (in Ref. 1; AAD12579)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 553 AA; 63041 MW; 5124EF8D8F14AEE9 CRC64;
MPHFNPVPVS NKKFVFDDFI LNMDGSLLRS EKKVNIPPKE YAVLVILLEA AGEIVSKNTL
LDQVWGDAEV NEESLTRCIY ALRRILSEDK EHRYIETLYG QGYRFNRPVV VVSPPAPQPT
THTLAILPFQ MQDQVQSESL HYSIVKGLSQ YAPFGLSVLP VTITKNCRSV KDILELMDQL
RPDYYISGQM IPDGNDNIVQ IEIVRVKGYH LLHQESIKLI EHQPASLLQN KIANLLLRCI
PGLRWDTKQI SELNSIDSTM VYLRGKHELN QYTPYSLQQA LKLLTQCVNM SPNSIAPYCA
LAECYLSMAQ MGIFDKQNAM IKAKEHAIKA TELDHNNPQA LGLLGLINTI HSEYIVGSLL
FKQANLLSPI SADIKYYYGW NLFMAGQLEE ALQTINECLK LDPTRAAAGI TKLWITYYHT
GIDDAIRLGD ELRSQHLQDN PILLSMQVMF LSLKGKHELA RKLTKEISTQ EITGLIAVNL
LYAEYCQNSE RALPTIREFL ESEQRIDNNP GLLPLVLVAH GEAIAEKMWN KFKNEDNIWF
KRWKQDPRLI KLR
