HIL_DROME
ID HIL_DROME Reviewed; 818 AA.
AC Q0E908; Q960G2;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Hillarin {ECO:0000312|FlyBase:FBgn0050147};
DE AltName: Full=D-Hillarin {ECO:0000303|PubMed:15818553};
DE Short=D-hil {ECO:0000303|PubMed:15818553};
GN Name=Hil {ECO:0000312|FlyBase:FBgn0050147};
GN ORFNames=CG30147 {ECO:0000312|FlyBase:FBgn0050147};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAK93501.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAK93501.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAK93501.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, INTERACTION WITH PNUT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=15818553; DOI=10.1002/neu.20131;
RA Ji Y., Rath U., Girton J., Johansen K.M., Johansen J.;
RT "D-Hillarin, a novel W180-domain protein, affects cytokinesis through
RT interaction with the septin family member Pnut.";
RL J. Neurobiol. 64:157-169(2005).
CC -!- FUNCTION: May act as a modulator of septin function during cytokinesis
CC in the developing nervous system. {ECO:0000303|PubMed:15818553}.
CC -!- SUBUNIT: Interacts with pnut. {ECO:0000269|PubMed:15818553}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex
CC {ECO:0000269|PubMed:15818553}. Cleavage furrow
CC {ECO:0000269|PubMed:15818553}. Note=During mitosis, detected at the
CC cell cortex in metaphase but is concentrated at the cleavage furrow at
CC telophase. Colocalizes with pnut at the cell cortex.
CC {ECO:0000269|PubMed:15818553}.
CC -!- TISSUE SPECIFICITY: Localizes to the neuropil of the embryonic central
CC nervous system (at protein level). Also detected in third instar larval
CC brain (at protein level). {ECO:0000269|PubMed:15818553}.
CC -!- DEVELOPMENTAL STAGE: Very low levels detected in 0-6 hour embryos with
CC expression up-regulated after 12 hours of embryogenesis and persisting
CC into adult stages (at protein level). {ECO:0000269|PubMed:15818553}.
CC -!- DISRUPTION PHENOTYPE: Mutants are viable with a 98.4% hatch rate which
CC is close to the wild-type hatch rate. No apparent defects in the
CC central nervous system. {ECO:0000269|PubMed:15818553}.
CC -!- SIMILARITY: Belongs to the transglutaminase-like superfamily.
CC {ECO:0000305}.
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DR EMBL; AE013599; AAF57448.2; -; Genomic_DNA.
DR EMBL; AE013599; AAF57449.2; -; Genomic_DNA.
DR EMBL; AY052077; AAK93501.1; -; mRNA.
DR RefSeq; NP_611493.2; NM_137649.3.
DR RefSeq; NP_725980.1; NM_166402.2.
DR AlphaFoldDB; Q0E908; -.
DR IntAct; Q0E908; 3.
DR STRING; 7227.FBpp0085567; -.
DR PaxDb; Q0E908; -.
DR PRIDE; Q0E908; -.
DR EnsemblMetazoa; FBtr0086255; FBpp0085567; FBgn0050147.
DR EnsemblMetazoa; FBtr0086256; FBpp0085568; FBgn0050147.
DR GeneID; 37328; -.
DR KEGG; dme:Dmel_CG30147; -.
DR UCSC; CG30147-RA; d. melanogaster.
DR UCSC; CG30147-RB; d. melanogaster.
DR CTD; 37328; -.
DR FlyBase; FBgn0050147; Hil.
DR VEuPathDB; VectorBase:FBgn0050147; -.
DR eggNOG; KOG1700; Eukaryota.
DR eggNOG; KOG4575; Eukaryota.
DR GeneTree; ENSGT00940000172737; -.
DR HOGENOM; CLU_009339_1_0_1; -.
DR InParanoid; Q0E908; -.
DR OMA; VAIHCAR; -.
DR OrthoDB; 936224at2759; -.
DR PhylomeDB; Q0E908; -.
DR BioGRID-ORCS; 37328; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37328; -.
DR PRO; PR:Q0E908; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0050147; Expressed in mouthpart and 22 other tissues.
DR GO; GO:0005938; C:cell cortex; IDA:UniProtKB.
DR GO; GO:0032154; C:cleavage furrow; IDA:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR002931; Transglutaminase-like.
DR InterPro; IPR001781; Znf_LIM.
DR SMART; SM00132; LIM; 1.
DR SMART; SM00460; TGc; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 1.
DR PROSITE; PS50023; LIM_DOMAIN_2; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; LIM domain; Metal-binding; Reference proteome;
KW Zinc.
FT CHAIN 1..818
FT /note="Hillarin"
FT /id="PRO_0000437138"
FT DOMAIN 9..76
FT /note="LIM zinc-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT REGION 97..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 216..272
FT /evidence="ECO:0000255"
FT COMPBIAS 114..141
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 788
FT /note="H -> Q (in Ref. 3; AAK93501)"
FT /evidence="ECO:0000305"
FT CONFLICT 794
FT /note="Missing (in Ref. 3; AAK93501)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 818 AA; 94465 MW; 4833D507336884F7 CRC64;
MYRPNFYEST CLRCSETVYQ VDRVGPLKDF TFFHSGCFKC VHCGTKLTLK TYFNNQHKQD
DKEVYCSSHV PKSGPGHLDQ TSVGIRQALN APRTNKFVNE QIRGTRSEVD GGPLGGSRQS
TPNGYGSREI SSPSQNDSDY KYGRFDASAL HIAHALKQTE IQKAYNKARE KPIDFYLARE
EQAHLEMKHR KEEDDLYRKF ASKRAEEDRK IQDEFQDEWE RELQRLTHKF EKELATSRRS
RDEANILTMR HEQQKEDLEK NMTLRRSKKK ESITRKMLEH ERYETAALVD RQSSEMLELI
SARRSEYMQS ESIFLDDEFS EGAVPVEYPL NAPIPAPPAV SKFQIYTDPI EFEDVDRIAI
SVAQEDQKTF TDLVRQLVGR CTTDIEKART IFRWITVKNL NAMHFDDDLR GDTPMGLLRG
IKYGTESYHV LFKRLCSYAG LHCVVIKGYS KSAGYQPGVK FQDSRFRNSW NAVYVAGAWR
FVQCNWGARH LVNAKEAPKQ GRGKNDSLRY EYDDHYFLTD PREFIYEFYP LQEEWQLLKR
PITLREFENL PFVRSLFFRY GLHFADEGYG AVVFTDDTGA ATVRIAMPTD MQSCLIFHYN
LKFYDSDEEL SYDGVSLKRF VMQSVIGNIV AFRVHAPCSG AFLLDIFANA VTPQEYLTGE
PMKFKSVCKF KICCEELQTV MVPLPDCASG EWGPTKATRL FGLIPITHQD PLIFAGRSLD
LQFRMSRPLT DFMATLHKNG IEEKKLAKYV THSTLDDIVT FIINFPEEGQ YGLDIYTREL
GGPQHHHHHN NNNSSSSSGE KHLLTHCCKY LINSSKRN
