HIM14_CAEEL
ID HIM14_CAEEL Reviewed; 842 AA.
AC Q23405;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=MutS protein homolog him-14;
DE AltName: Full=High incidence of males protein 14;
DE AltName: Full=MutS protein homolog 4;
GN Name=him-14 {ECO:0000312|WormBase:ZK1127.11};
GN Synonyms=msh-4 {ECO:0000303|PubMed:23832114};
GN ORFNames=ZK1127.11 {ECO:0000312|WormBase:ZK1127.11};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS
RP OF GLY-116; THR-238; ASP-406 AND SER-538.
RC STRAIN=Bristol N2;
RX PubMed=10545458; DOI=10.1093/genetics/153.3.1271;
RA Zalevsky J., MacQueen A.J., Duffy J.B., Kemphues K.J., Villeneuve A.M.;
RT "Crossing over during Caenorhabditis elegans meiosis requires a conserved
RT MutS-based pathway that is partially dispensable in budding yeast.";
RL Genetics 153:1271-1283(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP IDENTIFICATION.
RX PubMed=3224814; DOI=10.1093/genetics/120.4.977;
RA Kemphues K.J., Kusch M., Wolf N.;
RT "Maternal-effect lethal mutations on linkage group II of Caenorhabditis
RT elegans.";
RL Genetics 120:977-986(1988).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=8601478; DOI=10.1093/genetics/141.4.1339;
RA Zetka M.-C., Rose A.M.;
RT "Mutant rec-1 eliminates the meiotic pattern of crossing over in
RT Caenorhabditis elegans.";
RL Genetics 141:1339-1349(1995).
RN [5]
RP FUNCTION.
RX PubMed=16462941; DOI=10.1371/journal.pgen.0020012;
RA Carlton P.M., Farruggio A.P., Dernburg A.F.;
RT "A link between meiotic prophase progression and crossover control.";
RL PLoS Genet. 2:119-128(2006).
RN [6]
RP FUNCTION.
RX PubMed=23832114; DOI=10.1038/cdd.2013.68;
RA Silva N., Adamo A., Santonicola P., Martinez-Perez E., La Volpe A.;
RT "Pro-crossover factors regulate damage-dependent apoptosis in the
RT Caenorhabditis elegans germ line.";
RL Cell Death Differ. 20:1209-1218(2013).
CC -!- FUNCTION: Required during the pachytene stage of meiotic prophase for
CC the formation of crossovers between homologous chromosomes. Together
CC with msh-5 and zhp-3 plays a role in the activation of DNA damage-
CC dependent apoptosis at the DNA damage checkpoint in pachytene cells
CC (PubMed:23832114). Not needed for pairing or synapsis. May promote
CC crossing over by interfering with Holliday junction branch migration.
CC Has no apparent role in DNA mismatch repair.
CC {ECO:0000269|PubMed:10545458, ECO:0000269|PubMed:16462941,
CC ECO:0000269|PubMed:23832114, ECO:0000269|PubMed:8601478}.
CC -!- SUBUNIT: Heterooligomer of him-14 and msh-5.
CC {ECO:0000250|UniProtKB:P40965}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: Expressed maternally.
CC {ECO:0000269|PubMed:10545458}.
CC -!- DISRUPTION PHENOTYPE: Worms show maternal effect lethality producing
CC 95% inviable progeny from hermaphrodites. A high proportion of those
CC that survive are male. Mutants display severely reduced crossing over,
CC resulting in lack of chiasmata between homologous chromosomes and
CC consequent missegregation. {ECO:0000269|PubMed:8601478}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
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DR EMBL; AF178755; AAD52669.1; -; mRNA.
DR EMBL; BX284602; CCD73725.1; -; Genomic_DNA.
DR PIR; E88197; E88197.
DR RefSeq; NP_495451.1; NM_063050.5.
DR AlphaFoldDB; Q23405; -.
DR SMR; Q23405; -.
DR STRING; 6239.ZK1127.11; -.
DR PaxDb; Q23405; -.
DR PRIDE; Q23405; -.
DR EnsemblMetazoa; ZK1127.11.1; ZK1127.11.1; WBGene00001872.
DR GeneID; 174157; -.
DR KEGG; cel:CELE_ZK1127.11; -.
DR UCSC; ZK1127.11; c. elegans.
DR CTD; 174157; -.
DR WormBase; ZK1127.11; CE24714; WBGene00001872; him-14.
DR eggNOG; KOG0220; Eukaryota.
DR GeneTree; ENSGT00550000074897; -.
DR HOGENOM; CLU_002472_7_3_1; -.
DR InParanoid; Q23405; -.
DR OMA; AIAMLDM; -.
DR OrthoDB; 138168at2759; -.
DR PhylomeDB; Q23405; -.
DR PRO; PR:Q23405; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00001872; Expressed in germ line (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IMP:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0051026; P:chiasma assembly; IMP:WormBase.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0045143; P:homologous chromosome segregation; IMP:WormBase.
DR GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:WormBase.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IMP:UniProtKB.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR011184; DNA_mismatch_repair_Msh2.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF005813; MSH2; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA recombination; DNA-binding; Meiosis; Nucleotide-binding;
KW Nucleus; Reference proteome.
FT CHAIN 1..842
FT /note="MutS protein homolog him-14"
FT /id="PRO_0000115199"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 588..595
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MUTAGEN 116
FT /note="G->E: In me15; defective meiotic chromosomal
FT segregation."
FT /evidence="ECO:0000269|PubMed:10545458"
FT MUTAGEN 238
FT /note="T->I: In it13; defective meiotic chromosomal
FT segregation."
FT /evidence="ECO:0000269|PubMed:10545458"
FT MUTAGEN 406
FT /note="D->N: In it44; induces temperature sensitivity;
FT defective meiotic chromosomal segregation."
FT /evidence="ECO:0000269|PubMed:10545458"
FT MUTAGEN 538
FT /note="S->L: In it23; defective meiotic chromosomal
FT segregation."
FT /evidence="ECO:0000269|PubMed:10545458"
SQ SEQUENCE 842 AA; 96331 MW; 64D71CEF45F8157B CRC64;
MYSNKSFQRR QRQQVAESRS EEKFSRSLVR LNAQSLLDSS GNNTTTKNVN SDVVIVVMEG
RGSCEGHIGI ALHDTCFPEI HLCEFVDSRE YTTLKTMINV HEAFDIVIQN GNEERGSTKL
LGEALMTAFP EASLQSISSK YFNSERGERQ LQSLMNAEVS TVSEGCLRRT LALGALAVLL
KYIHETRCVF FRVKSLRIKE MGVNDTCMID FVSWESLEIV DADDASKARK FQMKQKRTLM
SVLNHTVTTN GYRLLRSSVL QPSTDVYLIQ SRQEAIEELI GKPQLKDKLR RTLSRAHELD
RVIAMCIQTS TSWTVRESEA KINQIIKLMH TLKVIQGIRT LLHSAKMKSN ILIEKTEFLK
DPRFDQIMNI LVEKVDDSLL DGKKNSLHLQ NTKCYAIRHF VAVQLDLARQ TYEEIIRNVE
ETGAREIAEY FHGNSSVRLS FSQSRGFHYT FVTRQAESVT IPRYFLDVFR NRTTVTFNSR
KVIAYNDRLE QVVAEMFLAS DVIVCDMIEE MQPMIPVLYY AMDALSSIDF LCGLATYSDL
RDTCKPTFGP SFSISQGRHP ILDWDDSEKT ITNDTCLTRD RRFGIITGPN MAGKSTYLKQ
TAQLAIMAQI GCFIPANYAS LPIFNRIFSR MGHNDELIRN KSAFASEMSD AAAIVQYADK
NSLVVLDELA RSTSTEEGIA ITYAICEKVL KLQSYTFLAT HFLDIAALAN YSNAIDNYHF
LPQTDENSTK KHKLLRGQYR GPLYGFELVE LSTIPDEVIE HAQSLATELR ANVEDTERDY
DSERRRIKVY MNHRFRECAE YFMDTHGEKW KEEKEAIDKM KALRKYLVDE LAKIDSQEQM
CQ
