HINA_HORVU
ID HINA_HORVU Reviewed; 149 AA.
AC Q9M4E3; Q0GJB4; Q0GJD7; Q0GJH0; Q0GJH1; Q8L567; Q8LK44; Q8LK45; Q8LK46;
AC Q8LSC9; Q8LSD0; Q8LSD1; Q8LSD2; Q8LSD3; Q8LSD4;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=Hordoindoline-A;
DE Flags: Precursor;
GN Name=HINA; Synonyms=HORDOA;
OS Hordeum vulgare (Barley).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX NCBI_TaxID=4513;
RN [1] {ECO:0000312|EMBL:CAB89519.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Leaf {ECO:0000312|EMBL:CAB89519.1};
RA Gautier M.-F., Cosson P., Guirao A., Alary R., Joudrier P.;
RT "Puroindoline genes are highly conserved in diploid ancestor wheats and
RT related species but absent in tetraploid Triticum species.";
RL Plant Sci. 153:81-91(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAM82592.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-149, AND VARIANTS VAL-9; LYS-29;
RP GLU-43; ARG-71; LYS-78; LEU-78; GLY-89; GLU-102; PRO-105 AND VAL-111.
RA Darlington Y.F., Giroux M.J., Shan X.;
RT "Identification of new barley hordoindoline alleles.";
RL Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAM82592.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-149, AND VARIANTS VAL-9; LYS-29;
RP GLU-43; ARG-71; LYS-78; LEU-78; GLY-89; GLU-102; PRO-105 AND VAL-111.
RC STRAIN=cv. Baronesse {ECO:0000312|EMBL:ABI18717.1},
RC cv. Chinook {ECO:0000312|EMBL:ABI18720.1},
RC cv. Harrington {ECO:0000312|EMBL:ABI18718.1},
RC cv. Landrace {ECO:0000312|EMBL:ABI18654.1},
RC cv. Lewis {ECO:0000312|EMBL:ABI18719.1},
RC cv. Medallion {ECO:0000312|EMBL:ABI18716.1},
RC cv. Merlin {ECO:0000312|EMBL:ABI18722.1},
RC cv. Morex {ECO:0000312|EMBL:ABI18721.1}, and
RC cv. Steptoe {ECO:0000312|EMBL:ABI18715.1};
RA Turuspekov Y., Beecher B., Darlington Y.F., Bowman J., Blake T.K.,
RA Giroux M.J.;
RT "The variation of hardness locus and endosperm texture in spring cultivars
RT of barley (Hordeum vulgare L.).";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=11785939; DOI=10.1023/a:1013691530675;
RA Darlington H.F., Rouster J., Hoffmann L., Halford N.G., Shewry P.R.,
RA Simpson D.J.;
RT "Identification and molecular characterisation of hordoindolines from
RT barley grain.";
RL Plant Mol. Biol. 47:785-794(2001).
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=12033628; DOI=10.1139/g02-008;
RA Beecher B., Bowman J., Martin J.M., Bettge A.D., Morris C.F., Blake T.K.,
RA Giroux M.J.;
RT "Hordoindolines are associated with a major endosperm-texture QTL in barley
RT (Hordeum vulgare).";
RL Genome 45:584-591(2002).
CC -!- FUNCTION: Acts as a membranotoxin, probably through its antibacterial
CC and antifungal activities, contributing to the defense mechanism of the
CC plant against predators. Forms monovalent cation-selective ion channels
CC in membranes (By similarity). Contributes to grain texture and
CC hardness. {ECO:0000250|UniProtKB:P33432, ECO:0000269|PubMed:12033628}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:P33432}.
CC Secreted, extracellular space {ECO:0000250|UniProtKB:P33432}.
CC -!- TISSUE SPECIFICITY: Found in endosperm and aleurone layer of developing
CC kernels, but not in the embryo. {ECO:0000269|PubMed:11785939}.
CC -!- PTM: Five disulfide bonds are present. {ECO:0000250|UniProtKB:P33432}.
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DR EMBL; AJ249929; CAB89519.1; -; Genomic_DNA.
DR EMBL; AF473870; AAM21044.1; -; Genomic_DNA.
DR EMBL; AF473871; AAM21045.1; -; Genomic_DNA.
DR EMBL; AF473872; AAM21046.1; -; Genomic_DNA.
DR EMBL; AF473873; AAM21047.1; -; Genomic_DNA.
DR EMBL; AF473874; AAM21048.1; -; Genomic_DNA.
DR EMBL; AF473875; AAM21049.1; -; Genomic_DNA.
DR EMBL; AF525034; AAM82592.1; -; Genomic_DNA.
DR EMBL; AF525035; AAM82593.1; -; Genomic_DNA.
DR EMBL; AF525036; AAM82594.1; -; Genomic_DNA.
DR EMBL; AF525037; AAM82595.1; -; Genomic_DNA.
DR EMBL; AF525038; AAM82596.1; -; Genomic_DNA.
DR EMBL; DQ862133; ABI18642.1; -; Genomic_DNA.
DR EMBL; DQ862134; ABI18643.1; -; Genomic_DNA.
DR EMBL; DQ862135; ABI18644.1; -; Genomic_DNA.
DR EMBL; DQ862136; ABI18645.1; -; Genomic_DNA.
DR EMBL; DQ862137; ABI18646.1; -; Genomic_DNA.
DR EMBL; DQ862138; ABI18647.1; -; Genomic_DNA.
DR EMBL; DQ862139; ABI18648.1; -; Genomic_DNA.
DR EMBL; DQ862140; ABI18649.1; -; Genomic_DNA.
DR EMBL; DQ862141; ABI18650.1; -; Genomic_DNA.
DR EMBL; DQ862142; ABI18651.1; -; Genomic_DNA.
DR EMBL; DQ862143; ABI18652.1; -; Genomic_DNA.
DR EMBL; DQ862144; ABI18653.1; -; Genomic_DNA.
DR EMBL; DQ862145; ABI18654.1; -; Genomic_DNA.
DR EMBL; DQ862146; ABI18655.1; -; Genomic_DNA.
DR EMBL; DQ862147; ABI18656.1; -; Genomic_DNA.
DR EMBL; DQ862148; ABI18657.1; -; Genomic_DNA.
DR EMBL; DQ862149; ABI18658.1; -; Genomic_DNA.
DR EMBL; DQ862150; ABI18659.1; -; Genomic_DNA.
DR EMBL; DQ862151; ABI18660.1; -; Genomic_DNA.
DR EMBL; DQ862152; ABI18661.1; -; Genomic_DNA.
DR EMBL; DQ862153; ABI18662.1; -; Genomic_DNA.
DR EMBL; DQ862154; ABI18663.1; -; Genomic_DNA.
DR EMBL; DQ862155; ABI18664.1; -; Genomic_DNA.
DR EMBL; DQ862156; ABI18665.1; -; Genomic_DNA.
DR EMBL; DQ862157; ABI18666.1; -; Genomic_DNA.
DR EMBL; DQ862158; ABI18667.1; -; Genomic_DNA.
DR EMBL; DQ862159; ABI18668.1; -; Genomic_DNA.
DR EMBL; DQ862160; ABI18669.1; -; Genomic_DNA.
DR EMBL; DQ862161; ABI18670.1; -; Genomic_DNA.
DR EMBL; DQ862162; ABI18671.1; -; Genomic_DNA.
DR EMBL; DQ862163; ABI18672.1; -; Genomic_DNA.
DR EMBL; DQ862164; ABI18673.1; -; Genomic_DNA.
DR EMBL; DQ862165; ABI18674.1; -; Genomic_DNA.
DR EMBL; DQ862166; ABI18675.1; -; Genomic_DNA.
DR EMBL; DQ862167; ABI18676.1; -; Genomic_DNA.
DR EMBL; DQ862168; ABI18677.1; -; Genomic_DNA.
DR EMBL; DQ862169; ABI18678.1; -; Genomic_DNA.
DR EMBL; DQ862170; ABI18679.1; -; Genomic_DNA.
DR EMBL; DQ862171; ABI18680.1; -; Genomic_DNA.
DR EMBL; DQ862172; ABI18681.1; -; Genomic_DNA.
DR EMBL; DQ862173; ABI18682.1; -; Genomic_DNA.
DR EMBL; DQ862174; ABI18683.1; -; Genomic_DNA.
DR EMBL; DQ862175; ABI18684.1; -; Genomic_DNA.
DR EMBL; DQ862176; ABI18685.1; -; Genomic_DNA.
DR EMBL; DQ862177; ABI18686.1; -; Genomic_DNA.
DR EMBL; DQ862178; ABI18687.1; -; Genomic_DNA.
DR EMBL; DQ862179; ABI18688.1; -; Genomic_DNA.
DR EMBL; DQ862180; ABI18689.1; -; Genomic_DNA.
DR EMBL; DQ862181; ABI18690.1; -; Genomic_DNA.
DR EMBL; DQ862182; ABI18691.1; -; Genomic_DNA.
DR EMBL; DQ862183; ABI18692.1; -; Genomic_DNA.
DR EMBL; DQ862184; ABI18693.1; -; Genomic_DNA.
DR EMBL; DQ862185; ABI18694.1; -; Genomic_DNA.
DR EMBL; DQ862186; ABI18695.1; -; Genomic_DNA.
DR EMBL; DQ862187; ABI18696.1; -; Genomic_DNA.
DR EMBL; DQ862188; ABI18697.1; -; Genomic_DNA.
DR EMBL; DQ862189; ABI18698.1; -; Genomic_DNA.
DR EMBL; DQ862190; ABI18699.1; -; Genomic_DNA.
DR EMBL; DQ862191; ABI18700.1; -; Genomic_DNA.
DR EMBL; DQ862192; ABI18701.1; -; Genomic_DNA.
DR EMBL; DQ862193; ABI18702.1; -; Genomic_DNA.
DR EMBL; DQ862194; ABI18703.1; -; Genomic_DNA.
DR EMBL; DQ862195; ABI18704.1; -; Genomic_DNA.
DR EMBL; DQ862196; ABI18705.1; -; Genomic_DNA.
DR EMBL; DQ862197; ABI18706.1; -; Genomic_DNA.
DR EMBL; DQ862198; ABI18707.1; -; Genomic_DNA.
DR EMBL; DQ862199; ABI18708.1; -; Genomic_DNA.
DR EMBL; DQ862200; ABI18709.1; -; Genomic_DNA.
DR EMBL; DQ862201; ABI18710.1; -; Genomic_DNA.
DR EMBL; DQ862202; ABI18711.1; -; Genomic_DNA.
DR EMBL; DQ862203; ABI18712.1; -; Genomic_DNA.
DR EMBL; DQ862204; ABI18713.1; -; Genomic_DNA.
DR EMBL; DQ862205; ABI18714.1; -; Genomic_DNA.
DR EMBL; DQ862206; ABI18715.1; -; Genomic_DNA.
DR EMBL; DQ862207; ABI18716.1; -; Genomic_DNA.
DR EMBL; DQ862208; ABI18717.1; -; Genomic_DNA.
DR EMBL; DQ862209; ABI18718.1; -; Genomic_DNA.
DR EMBL; DQ862210; ABI18719.1; -; Genomic_DNA.
DR EMBL; DQ862211; ABI18720.1; -; Genomic_DNA.
DR EMBL; DQ862212; ABI18721.1; -; Genomic_DNA.
DR EMBL; DQ862213; ABI18722.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9M4E3; -.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR001954; Glia_glutenin.
DR PANTHER; PTHR33454; PTHR33454; 1.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 2: Evidence at transcript level;
KW Antibiotic; Antimicrobial; Disulfide bond; Membrane; Plant defense;
KW Secreted; Signal; Toxin.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..28
FT /evidence="ECO:0000250|UniProtKB:P33432"
FT /id="PRO_0000309561"
FT CHAIN 29..147
FT /note="Hordoindoline-A"
FT /evidence="ECO:0000250|UniProtKB:P33432"
FT /id="PRO_5000065690"
FT PROPEP 148..149
FT /note="Removed in mature form"
FT /evidence="ECO:0000250|UniProtKB:P33432"
FT /id="PRO_0000309562"
FT VARIANT 9
FT /note="L -> V"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT VARIANT 29
FT /note="E -> K"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT VARIANT 43
FT /note="G -> E"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT VARIANT 71
FT /note="T -> R"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT VARIANT 78
FT /note="E -> K"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT VARIANT 78
FT /note="E -> L"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT VARIANT 89
FT /note="S -> G"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT VARIANT 102
FT /note="G -> E"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT VARIANT 105
FT /note="Q -> P"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT VARIANT 111
FT /note="F -> V"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
SQ SEQUENCE 149 AA; 16494 MW; 5B7F760EF11DAA2B CRC64;
MKALFLMGLL ALVASAAFAQ YGEVVGSYEG GAGGGGAQQC PLGTKLDSCR NYLLDRCTTM
KDFPVTWRWW TWWKGGCEEL LHDCCSQLSQ MPPQCRCNII QGSIQRDLGG FFGFQRDRTV
KVIQAAKNLP PRCNQGPACN IPSTIGYYW
