HINB1_HORVU
ID HINB1_HORVU Reviewed; 147 AA.
AC Q9FSI9; P82940; Q0GJ26; Q0GJ42; Q0GJ69; Q0GJ83; Q0GJ91;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Hordoindoline-B1;
DE AltName: Full=Puroindoline-B;
DE Flags: Precursor;
GN Name=HINB-1; Synonyms=HOI-B1;
OS Hordeum vulgare (Barley).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX NCBI_TaxID=4513;
RN [1] {ECO:0000305, ECO:0000312|EMBL:CAB92291.2}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 29-38, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Chariot {ECO:0000269|PubMed:11785939}, and
RC cv. Igri {ECO:0000312|EMBL:CAB92291.2};
RC TISSUE=Leaf {ECO:0000312|EMBL:CAB92291.2}, and
RC Seed {ECO:0000269|PubMed:11785939};
RX PubMed=11785939; DOI=10.1023/a:1013691530675;
RA Darlington H.F., Rouster J., Hoffmann L., Halford N.G., Shewry P.R.,
RA Simpson D.J.;
RT "Identification and molecular characterisation of hordoindolines from
RT barley grain.";
RL Plant Mol. Biol. 47:785-794(2001).
RN [2] {ECO:0000305, ECO:0000312|EMBL:ABI18726.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS LEU-9; HIS-41; ARG-59;
RP HIS-122 AND LEU-141.
RC STRAIN=cv. Baronesse {ECO:0000312|EMBL:ABI18798.1},
RC cv. Chinook {ECO:0000312|EMBL:ABI18801.1},
RC cv. Harrington {ECO:0000312|EMBL:ABI18799.1},
RC cv. Landrace {ECO:0000312|EMBL:ABI18726.1},
RC cv. Lewis {ECO:0000312|EMBL:ABI18800.1},
RC cv. Medallion {ECO:0000312|EMBL:ABI18797.1},
RC cv. Merlin {ECO:0000312|EMBL:ABI18803.1},
RC cv. Morex {ECO:0000312|EMBL:ABI18802.1}, and
RC cv. Steptoe {ECO:0000312|EMBL:ABI18796.1};
RA Turuspekov Y., Beecher B., Darlington Y.F., Bowman J., Blake T.K.,
RA Giroux M.J.;
RT "The variation of hardness locus and endosperm texture in spring cultivars
RT of barley (Hordeum vulgare L.).";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP PROTEIN SEQUENCE OF 72-80.
RC STRAIN=cv. Bomi {ECO:0000269|PubMed:11271488};
RC TISSUE=Starchy endosperm {ECO:0000269|PubMed:11271488};
RX PubMed=11271488;
RX DOI=10.1002/1522-2683(200011)21:17<3693::aid-elps3693>3.0.co;2-i;
RA Kristoffersen H.E., Flengsrud R.;
RT "Separation and characterization of basic barley seed proteins.";
RL Electrophoresis 21:3693-3700(2000).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=12033628; DOI=10.1139/g02-008;
RA Beecher B., Bowman J., Martin J.M., Bettge A.D., Morris C.F., Blake T.K.,
RA Giroux M.J.;
RT "Hordoindolines are associated with a major endosperm-texture QTL in barley
RT (Hordeum vulgare).";
RL Genome 45:584-591(2002).
CC -!- FUNCTION: Acts as a membranotoxin, probably through its antibacterial
CC and antifungal activities, contributing to the defense mechanism of the
CC plant against predators. Forms monovalent cation-selective ion channels
CC in membranes (By similarity). Contributes to grain texture and
CC hardness. {ECO:0000250|UniProtKB:Q10464, ECO:0000269|PubMed:12033628}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q10464}.
CC Secreted, extracellular space {ECO:0000250|UniProtKB:Q10464}.
CC -!- TISSUE SPECIFICITY: Found in endosperm and aleurone layer of developing
CC kernels, but not in the embryo. {ECO:0000269|PubMed:11785939}.
CC -!- PTM: Five disulfide bonds are present. {ECO:0000250|UniProtKB:Q10464}.
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DR EMBL; AJ276143; CAB92291.2; -; Genomic_DNA.
DR EMBL; DQ862214; ABI18723.1; -; Genomic_DNA.
DR EMBL; DQ862215; ABI18724.1; -; Genomic_DNA.
DR EMBL; DQ862216; ABI18725.1; -; Genomic_DNA.
DR EMBL; DQ862217; ABI18726.1; -; Genomic_DNA.
DR EMBL; DQ862218; ABI18727.1; -; Genomic_DNA.
DR EMBL; DQ862219; ABI18728.1; -; Genomic_DNA.
DR EMBL; DQ862220; ABI18729.1; -; Genomic_DNA.
DR EMBL; DQ862221; ABI18730.1; -; Genomic_DNA.
DR EMBL; DQ862222; ABI18731.1; -; Genomic_DNA.
DR EMBL; DQ862223; ABI18732.1; -; Genomic_DNA.
DR EMBL; DQ862224; ABI18733.1; -; Genomic_DNA.
DR EMBL; DQ862225; ABI18734.1; -; Genomic_DNA.
DR EMBL; DQ862226; ABI18735.1; -; Genomic_DNA.
DR EMBL; DQ862227; ABI18736.1; -; Genomic_DNA.
DR EMBL; DQ862228; ABI18737.1; -; Genomic_DNA.
DR EMBL; DQ862229; ABI18738.1; -; Genomic_DNA.
DR EMBL; DQ862230; ABI18739.1; -; Genomic_DNA.
DR EMBL; DQ862231; ABI18740.1; -; Genomic_DNA.
DR EMBL; DQ862232; ABI18741.1; -; Genomic_DNA.
DR EMBL; DQ862233; ABI18742.1; -; Genomic_DNA.
DR EMBL; DQ862234; ABI18743.1; -; Genomic_DNA.
DR EMBL; DQ862235; ABI18744.1; -; Genomic_DNA.
DR EMBL; DQ862236; ABI18745.1; -; Genomic_DNA.
DR EMBL; DQ862237; ABI18746.1; -; Genomic_DNA.
DR EMBL; DQ862238; ABI18747.1; -; Genomic_DNA.
DR EMBL; DQ862239; ABI18748.1; -; Genomic_DNA.
DR EMBL; DQ862240; ABI18749.1; -; Genomic_DNA.
DR EMBL; DQ862241; ABI18750.1; -; Genomic_DNA.
DR EMBL; DQ862242; ABI18751.1; -; Genomic_DNA.
DR EMBL; DQ862243; ABI18752.1; -; Genomic_DNA.
DR EMBL; DQ862244; ABI18753.1; -; Genomic_DNA.
DR EMBL; DQ862245; ABI18754.1; -; Genomic_DNA.
DR EMBL; DQ862246; ABI18755.1; -; Genomic_DNA.
DR EMBL; DQ862247; ABI18756.1; -; Genomic_DNA.
DR EMBL; DQ862248; ABI18757.1; -; Genomic_DNA.
DR EMBL; DQ862249; ABI18758.1; -; Genomic_DNA.
DR EMBL; DQ862250; ABI18759.1; -; Genomic_DNA.
DR EMBL; DQ862251; ABI18760.1; -; Genomic_DNA.
DR EMBL; DQ862252; ABI18761.1; -; Genomic_DNA.
DR EMBL; DQ862253; ABI18762.1; -; Genomic_DNA.
DR EMBL; DQ862254; ABI18763.1; -; Genomic_DNA.
DR EMBL; DQ862255; ABI18764.1; -; Genomic_DNA.
DR EMBL; DQ862256; ABI18765.1; -; Genomic_DNA.
DR EMBL; DQ862257; ABI18766.1; -; Genomic_DNA.
DR EMBL; DQ862258; ABI18767.1; -; Genomic_DNA.
DR EMBL; DQ862259; ABI18768.1; -; Genomic_DNA.
DR EMBL; DQ862260; ABI18769.1; -; Genomic_DNA.
DR EMBL; DQ862261; ABI18770.1; -; Genomic_DNA.
DR EMBL; DQ862262; ABI18771.1; -; Genomic_DNA.
DR EMBL; DQ862263; ABI18772.1; -; Genomic_DNA.
DR EMBL; DQ862264; ABI18773.1; -; Genomic_DNA.
DR EMBL; DQ862265; ABI18774.1; -; Genomic_DNA.
DR EMBL; DQ862266; ABI18775.1; -; Genomic_DNA.
DR EMBL; DQ862267; ABI18776.1; -; Genomic_DNA.
DR EMBL; DQ862268; ABI18777.1; -; Genomic_DNA.
DR EMBL; DQ862269; ABI18778.1; -; Genomic_DNA.
DR EMBL; DQ862270; ABI18779.1; -; Genomic_DNA.
DR EMBL; DQ862271; ABI18780.1; -; Genomic_DNA.
DR EMBL; DQ862272; ABI18781.1; -; Genomic_DNA.
DR EMBL; DQ862273; ABI18782.1; -; Genomic_DNA.
DR EMBL; DQ862274; ABI18783.1; -; Genomic_DNA.
DR EMBL; DQ862275; ABI18784.1; -; Genomic_DNA.
DR EMBL; DQ862276; ABI18785.1; -; Genomic_DNA.
DR EMBL; DQ862277; ABI18786.1; -; Genomic_DNA.
DR EMBL; DQ862278; ABI18787.1; -; Genomic_DNA.
DR EMBL; DQ862279; ABI18788.1; -; Genomic_DNA.
DR EMBL; DQ862280; ABI18789.1; -; Genomic_DNA.
DR EMBL; DQ862281; ABI18790.1; -; Genomic_DNA.
DR EMBL; DQ862282; ABI18791.1; -; Genomic_DNA.
DR EMBL; DQ862283; ABI18792.1; -; Genomic_DNA.
DR EMBL; DQ862284; ABI18793.1; -; Genomic_DNA.
DR EMBL; DQ862285; ABI18794.1; -; Genomic_DNA.
DR EMBL; DQ862286; ABI18795.1; -; Genomic_DNA.
DR EMBL; DQ862287; ABI18796.1; -; Genomic_DNA.
DR EMBL; DQ862288; ABI18797.1; -; Genomic_DNA.
DR EMBL; DQ862289; ABI18798.1; -; Genomic_DNA.
DR EMBL; DQ862290; ABI18799.1; -; Genomic_DNA.
DR EMBL; DQ862291; ABI18800.1; -; Genomic_DNA.
DR EMBL; DQ862292; ABI18801.1; -; Genomic_DNA.
DR EMBL; DQ862293; ABI18802.1; -; Genomic_DNA.
DR EMBL; DQ862294; ABI18803.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9FSI9; -.
DR ExpressionAtlas; Q9FSI9; baseline and differential.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:InterPro.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR001954; Glia_glutenin.
DR PANTHER; PTHR33454; PTHR33454; 1.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PRINTS; PR00808; AMLASEINHBTR.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW Membrane; Plant defense; Secreted; Signal; Toxin.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..28
FT /evidence="ECO:0000250|UniProtKB:Q10464"
FT /id="PRO_0000309563"
FT CHAIN 29..147
FT /note="Hordoindoline-B1"
FT /id="PRO_0000309564"
FT VARIANT 9
FT /note="I -> L"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 16
FT /note="T -> I"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 41
FT /note="Q -> H"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 59
FT /note="T -> R"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 122
FT /note="Q -> H"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 141
FT /note="F -> L"
FT /evidence="ECO:0000269|Ref.2"
FT CONFLICT 72
FT /note="W -> M (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 147 AA; 16122 MW; 57871761245CDD40 CRC64;
MKTLFLLAIL ALVASTTFAQ YSVGGGYNDV GGGGGSQQCP QERPNLGSCK DYVMERCFTM
KDFPLTWPTK WWKGGCEQEV REKCCQQLSQ IAPQCRCDAI RGVIQGKLGG IFGIGGGDVF
KQIQRAQILP SKCNMGADCK FPSGYYW
