HINB2_HORVU
ID HINB2_HORVU Reviewed; 147 AA.
AC Q9LEH8; P82940; Q0GIU5; Q0GIU7; Q0GIU9; Q0GIV7; Q0GIW8; Q0GIX2; Q0GIY1;
AC Q0GJ02; Q0GJ16; Q9LE34; Q9M3V3; Q9M3V4; Q9M3V5; Q9M3V6;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Hordoindoline-B2;
DE AltName: Full=Puroindoline-B;
DE Flags: Precursor;
GN Name=HINB-2; Synonyms=HOI-B2;
OS Hordeum vulgare (Barley).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX NCBI_TaxID=4513;
RN [1] {ECO:0000305, ECO:0000312|EMBL:CAB92292.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 29-38, TISSUE
RP SPECIFICITY, AND VARIANTS PRO-22; LYS-82 AND VAL-123.
RC STRAIN=cv. Britannia {ECO:0000312|EMBL:CAB90636.1},
RC cv. Chariot {ECO:0000269|PubMed:11785939, ECO:0000312|EMBL:CAB90637.1},
RC cv. Derkado {ECO:0000312|EMBL:CAB90639.1},
RC cv. Hart {ECO:0000312|EMBL:CAB90640.1},
RC cv. Igri {ECO:0000312|EMBL:CAB92292.1},
RC cv. Sundance {ECO:0000312|EMBL:CAB90641.1}, and
RC cv. Triumph {ECO:0000312|EMBL:CAB90638.1};
RC TISSUE=Endosperm {ECO:0000312|EMBL:CAB90641.1},
RC Leaf {ECO:0000312|EMBL:CAB92292.1}, and Seed {ECO:0000269|PubMed:11785939};
RX PubMed=11785939; DOI=10.1023/a:1013691530675;
RA Darlington H.F., Rouster J., Hoffmann L., Halford N.G., Shewry P.R.,
RA Simpson D.J.;
RT "Identification and molecular characterisation of hordoindolines from
RT barley grain.";
RL Plant Mol. Biol. 47:785-794(2001).
RN [2] {ECO:0000305, ECO:0000312|EMBL:ABI18812.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS LEU-5; ILE-6; LEU-64;
RP MET-88; GLN-94; ASP-118; ALA-137 AND LYS-140.
RC STRAIN=cv. Baronesse {ECO:0000312|EMBL:ABI18879.1},
RC cv. Chinook {ECO:0000312|EMBL:ABI18882.1},
RC cv. Harrington {ECO:0000312|EMBL:ABI18880.1},
RC cv. Landrace {ECO:0000312|EMBL:ABI18812.1},
RC cv. Lewis {ECO:0000312|EMBL:ABI18881.1},
RC cv. Medallion {ECO:0000312|EMBL:ABI18878.1},
RC cv. Merlin {ECO:0000312|EMBL:ABI18884.1},
RC cv. Morex {ECO:0000312|EMBL:ABI18883.1}, and
RC cv. Steptoe {ECO:0000312|EMBL:ABI18877.1};
RA Turuspekov Y., Beecher B., Darlington Y.F., Bowman J., Blake T.K.,
RA Giroux M.J.;
RT "The variation of hardness locus and endosperm texture in spring cultivars
RT of barley (Hordeum vulgare L.).";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP PROTEIN SEQUENCE OF 72-82.
RC STRAIN=cv. Bomi {ECO:0000269|PubMed:11271488};
RC TISSUE=Starchy endosperm {ECO:0000269|PubMed:11271488};
RX PubMed=11271488;
RX DOI=10.1002/1522-2683(200011)21:17<3693::aid-elps3693>3.0.co;2-i;
RA Kristoffersen H.E., Flengsrud R.;
RT "Separation and characterization of basic barley seed proteins.";
RL Electrophoresis 21:3693-3700(2000).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=12033628; DOI=10.1139/g02-008;
RA Beecher B., Bowman J., Martin J.M., Bettge A.D., Morris C.F., Blake T.K.,
RA Giroux M.J.;
RT "Hordoindolines are associated with a major endosperm-texture QTL in barley
RT (Hordeum vulgare).";
RL Genome 45:584-591(2002).
CC -!- FUNCTION: Acts as a membranotoxin, probably through its antibacterial
CC and antifungal activities, contributing to the defense mechanism of the
CC plant against predators. Forms monovalent cation-selective ion channels
CC in membranes (By similarity). Contributes to grain texture and
CC hardness. {ECO:0000250|UniProtKB:Q10464, ECO:0000269|PubMed:12033628}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q10464}.
CC Secreted, extracellular space {ECO:0000250|UniProtKB:Q10464}.
CC -!- TISSUE SPECIFICITY: Found in endosperm and aleurone layer of developing
CC kernels, but not in the embryo. {ECO:0000269|PubMed:11785939}.
CC -!- PTM: Five disulfide bonds are present. {ECO:0000250|UniProtKB:Q10464}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB90636.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAB90637.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAB90638.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAB90639.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAB90640.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAB90641.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ276143; CAB92292.1; -; Genomic_DNA.
DR EMBL; AJ277548; CAB90636.1; ALT_INIT; mRNA.
DR EMBL; AJ277549; CAB90637.1; ALT_INIT; mRNA.
DR EMBL; AJ277550; CAB90638.1; ALT_INIT; mRNA.
DR EMBL; AJ277551; CAB90639.1; ALT_INIT; mRNA.
DR EMBL; AJ277552; CAB90640.1; ALT_INIT; mRNA.
DR EMBL; AJ277553; CAB90641.1; ALT_INIT; mRNA.
DR EMBL; DQ862295; ABI18804.1; -; Genomic_DNA.
DR EMBL; DQ862296; ABI18805.1; -; Genomic_DNA.
DR EMBL; DQ862297; ABI18806.1; -; Genomic_DNA.
DR EMBL; DQ862298; ABI18807.1; -; Genomic_DNA.
DR EMBL; DQ862299; ABI18808.1; -; Genomic_DNA.
DR EMBL; DQ862300; ABI18809.1; -; Genomic_DNA.
DR EMBL; DQ862301; ABI18810.1; -; Genomic_DNA.
DR EMBL; DQ862302; ABI18811.1; -; Genomic_DNA.
DR EMBL; DQ862303; ABI18812.1; -; Genomic_DNA.
DR EMBL; DQ862304; ABI18813.1; -; Genomic_DNA.
DR EMBL; DQ862305; ABI18814.1; -; Genomic_DNA.
DR EMBL; DQ862306; ABI18815.1; -; Genomic_DNA.
DR EMBL; DQ862307; ABI18816.1; -; Genomic_DNA.
DR EMBL; DQ862308; ABI18817.1; -; Genomic_DNA.
DR EMBL; DQ862309; ABI18818.1; -; Genomic_DNA.
DR EMBL; DQ862310; ABI18819.1; -; Genomic_DNA.
DR EMBL; DQ862311; ABI18820.1; -; Genomic_DNA.
DR EMBL; DQ862312; ABI18821.1; -; Genomic_DNA.
DR EMBL; DQ862313; ABI18822.1; -; Genomic_DNA.
DR EMBL; DQ862314; ABI18823.1; -; Genomic_DNA.
DR EMBL; DQ862315; ABI18824.1; -; Genomic_DNA.
DR EMBL; DQ862316; ABI18825.1; -; Genomic_DNA.
DR EMBL; DQ862317; ABI18826.1; -; Genomic_DNA.
DR EMBL; DQ862318; ABI18827.1; -; Genomic_DNA.
DR EMBL; DQ862319; ABI18828.1; -; Genomic_DNA.
DR EMBL; DQ862320; ABI18829.1; -; Genomic_DNA.
DR EMBL; DQ862321; ABI18830.1; -; Genomic_DNA.
DR EMBL; DQ862322; ABI18831.1; -; Genomic_DNA.
DR EMBL; DQ862323; ABI18832.1; -; Genomic_DNA.
DR EMBL; DQ862324; ABI18833.1; -; Genomic_DNA.
DR EMBL; DQ862325; ABI18834.1; -; Genomic_DNA.
DR EMBL; DQ862326; ABI18835.1; -; Genomic_DNA.
DR EMBL; DQ862327; ABI18836.1; -; Genomic_DNA.
DR EMBL; DQ862328; ABI18837.1; -; Genomic_DNA.
DR EMBL; DQ862329; ABI18838.1; -; Genomic_DNA.
DR EMBL; DQ862330; ABI18839.1; -; Genomic_DNA.
DR EMBL; DQ862331; ABI18840.1; -; Genomic_DNA.
DR EMBL; DQ862332; ABI18841.1; -; Genomic_DNA.
DR EMBL; DQ862333; ABI18842.1; -; Genomic_DNA.
DR EMBL; DQ862334; ABI18843.1; -; Genomic_DNA.
DR EMBL; DQ862335; ABI18844.1; -; Genomic_DNA.
DR EMBL; DQ862336; ABI18845.1; -; Genomic_DNA.
DR EMBL; DQ862337; ABI18846.1; -; Genomic_DNA.
DR EMBL; DQ862338; ABI18847.1; -; Genomic_DNA.
DR EMBL; DQ862339; ABI18848.1; -; Genomic_DNA.
DR EMBL; DQ862340; ABI18849.1; -; Genomic_DNA.
DR EMBL; DQ862341; ABI18850.1; -; Genomic_DNA.
DR EMBL; DQ862342; ABI18851.1; -; Genomic_DNA.
DR EMBL; DQ862343; ABI18852.1; -; Genomic_DNA.
DR EMBL; DQ862344; ABI18853.1; -; Genomic_DNA.
DR EMBL; DQ862345; ABI18854.1; -; Genomic_DNA.
DR EMBL; DQ862346; ABI18855.1; -; Genomic_DNA.
DR EMBL; DQ862347; ABI18856.1; -; Genomic_DNA.
DR EMBL; DQ862348; ABI18857.1; -; Genomic_DNA.
DR EMBL; DQ862349; ABI18858.1; -; Genomic_DNA.
DR EMBL; DQ862350; ABI18859.1; -; Genomic_DNA.
DR EMBL; DQ862351; ABI18860.1; -; Genomic_DNA.
DR EMBL; DQ862352; ABI18861.1; -; Genomic_DNA.
DR EMBL; DQ862353; ABI18862.1; -; Genomic_DNA.
DR EMBL; DQ862354; ABI18863.1; -; Genomic_DNA.
DR EMBL; DQ862355; ABI18864.1; -; Genomic_DNA.
DR EMBL; DQ862356; ABI18865.1; -; Genomic_DNA.
DR EMBL; DQ862357; ABI18866.1; -; Genomic_DNA.
DR EMBL; DQ862358; ABI18867.1; -; Genomic_DNA.
DR EMBL; DQ862359; ABI18868.1; -; Genomic_DNA.
DR EMBL; DQ862360; ABI18869.1; -; Genomic_DNA.
DR EMBL; DQ862361; ABI18870.1; -; Genomic_DNA.
DR EMBL; DQ862362; ABI18871.1; -; Genomic_DNA.
DR EMBL; DQ862363; ABI18872.1; -; Genomic_DNA.
DR EMBL; DQ862364; ABI18873.1; -; Genomic_DNA.
DR EMBL; DQ862365; ABI18874.1; -; Genomic_DNA.
DR EMBL; DQ862366; ABI18875.1; -; Genomic_DNA.
DR EMBL; DQ862367; ABI18876.1; -; Genomic_DNA.
DR EMBL; DQ862368; ABI18877.1; -; Genomic_DNA.
DR EMBL; DQ862369; ABI18878.1; -; Genomic_DNA.
DR EMBL; DQ862370; ABI18879.1; -; Genomic_DNA.
DR EMBL; DQ862371; ABI18880.1; -; Genomic_DNA.
DR EMBL; DQ862372; ABI18881.1; -; Genomic_DNA.
DR EMBL; DQ862373; ABI18882.1; -; Genomic_DNA.
DR EMBL; DQ862374; ABI18883.1; -; Genomic_DNA.
DR EMBL; DQ862375; ABI18884.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9LEH8; -.
DR EnsemblPlants; HORVU.MOREX.r2.5HG0349700.1.mrna1; HORVU.MOREX.r2.5HG0349700.1.mrna1.cds1; HORVU.MOREX.r2.5HG0349700.1.
DR Gramene; HORVU.MOREX.r2.5HG0349700.1.mrna1; HORVU.MOREX.r2.5HG0349700.1.mrna1.cds1; HORVU.MOREX.r2.5HG0349700.1.
DR ExpressionAtlas; Q9LEH8; baseline and differential.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:InterPro.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR001954; Glia_glutenin.
DR PANTHER; PTHR33454; PTHR33454; 1.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PRINTS; PR00808; AMLASEINHBTR.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW Membrane; Plant defense; Secreted; Signal; Toxin.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..28
FT /evidence="ECO:0000250|UniProtKB:Q10464"
FT /id="PRO_0000309565"
FT CHAIN 29..147
FT /note="Hordoindoline-B2"
FT /id="PRO_0000309566"
FT VARIANT 5
FT /note="F -> L"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 6
FT /note="L -> I"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 22
FT /note="S -> P"
FT /evidence="ECO:0000269|PubMed:11785939"
FT VARIANT 64
FT /note="P -> L"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 82
FT /note="E -> K"
FT /evidence="ECO:0000269|PubMed:11785939"
FT VARIANT 88
FT /note="L -> M"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 94
FT /note="H -> Q"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 118
FT /note="A -> D"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 123
FT /note="I -> V"
FT /evidence="ECO:0000269|PubMed:11785939"
FT VARIANT 137
FT /note="V -> A"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 140
FT /note="R -> K"
FT /evidence="ECO:0000269|Ref.2"
FT CONFLICT 72
FT /note="W -> M (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 147 AA; 16078 MW; C106EBDB5A7CFD22 CRC64;
MKTLFLLALL ALVASTTSAQ YSVGGGYNDV GGGGGSQQCP QERPNLGSCK DYVMERCFTM
KDFPVTWPTK WWKGGCEHEV REKCCQQLSQ IAPHCRCDAI RGVIQGKLGG IFGIGGGAVF
KQIQRAQILP SKCNMGVDCR FPSGYYW
