ID   HINT3_XENTR             Reviewed;         153 AA.
AC   Q28BZ2;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Adenosine 5'-monophosphoramidase HINT3 {ECO:0000250|UniProtKB:Q9NQE9};
DE            EC=3.9.1.- {ECO:0000250|UniProtKB:Q9NQE9};
DE   AltName: Full=Histidine triad nucleotide-binding protein 3;
DE            Short=HINT-3;
GN   Name=hint3; ORFNames=TEgg107p05.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Egg;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Exhibits adenosine 5'-monophosphoramidase activity,
CC       hydrolyzing purine nucleotide phosphoramidates with a single phosphate
CC       group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP
CC       and NH2 (By similarity). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-
CC       acetyl lysine methyl ester)) generated by lysine tRNA ligase (By
CC       similarity). {ECO:0000250|UniProtKB:Q9NQE9}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine 5'-phosphoramidate + H2O = AMP + NH4(+);
CC         Xref=Rhea:RHEA:67916, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:57890, ChEBI:CHEBI:456215;
CC         Evidence={ECO:0000250|UniProtKB:Q9NQE9};
CC   -!- SUBUNIT: Forms dimers to octamers and even larger oligomer.
CC       {ECO:0000250|UniProtKB:Q9NQE9}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9NQE9}. Nucleus
CC       {ECO:0000250|UniProtKB:Q9NQE9}.
CC   -!- SIMILARITY: Belongs to the HINT family. {ECO:0000305}.
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DR   EMBL; CR942552; CAJ83661.1; -; mRNA.
DR   EMBL; BC158181; AAI58182.1; -; mRNA.
DR   RefSeq; NP_001039146.1; NM_001045681.1.
DR   AlphaFoldDB; Q28BZ2; -.
DR   SMR; Q28BZ2; -.
DR   STRING; 8364.ENSXETP00000042472; -.
DR   PaxDb; Q28BZ2; -.
DR   Ensembl; ENSXETT00000079777; ENSXETP00000079181; ENSXETG00000040798.
DR   GeneID; 733971; -.
DR   KEGG; xtr:733971; -.
DR   CTD; 135114; -.
DR   Xenbase; XB-GENE-1000078; hint3.
DR   eggNOG; KOG4359; Eukaryota.
DR   HOGENOM; CLU_056776_4_2_1; -.
DR   InParanoid; Q28BZ2; -.
DR   OMA; DFKNTRM; -.
DR   OrthoDB; 1496385at2759; -.
DR   PhylomeDB; Q28BZ2; -.
DR   TreeFam; TF353069; -.
DR   Proteomes; UP000008143; Chromosome 5.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000040798; Expressed in skeletal muscle tissue and 13 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0043530; F:adenosine 5'-monophosphoramidase activity; ISS:UniProtKB.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.428.10; -; 1.
DR   InterPro; IPR011146; HIT-like.
DR   InterPro; IPR036265; HIT-like_sf.
DR   SUPFAM; SSF54197; SSF54197; 1.
DR   PROSITE; PS51084; HIT_2; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN           1..153
FT                   /note="Adenosine 5'-monophosphoramidase HINT3"
FT                   /id="PRO_0000324332"
FT   DOMAIN          20..130
FT                   /note="HIT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00464"
FT   MOTIF           113..117
FT                   /note="Histidine triad motif"
FT   ACT_SITE        115
FT                   /note="Tele-AMP-histidine intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NQE9"
FT   BINDING         46..47
FT                   /ligand="AMP"
FT                   /ligand_id="ChEBI:CHEBI:456215"
FT                   /evidence="ECO:0000250|UniProtKB:P49773"
FT   BINDING         115..117
FT                   /ligand="AMP"
FT                   /ligand_id="ChEBI:CHEBI:456215"
FT                   /evidence="ECO:0000250|UniProtKB:P49773"
SQ   SEQUENCE   153 AA;  17316 MW;  A9767B44AA797455 CRC64;
     MSAEAGATVE QNHSYDMSCI FCRIANKQES GAELLHSDDD LVCFKDIRPA VTHHYLVVPK
     KHVGTCKTLT KDHVQLIKTM MEVGKSTLQK NNVTDLEDIR LGFHYPPFCS ISHLHLHVLA
     PASQLGFLSR MIYRVNSYWF ITADELIDQL QAS