HINT4_ARATH
ID HINT4_ARATH Reviewed; 146 AA.
AC Q84VV6; O23500; Q8L9E2;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 {ECO:0000305|PubMed:19896942};
DE Short=APS phosphorylase {ECO:0000305|PubMed:19896942};
DE EC=2.7.7.5 {ECO:0000269|PubMed:19896942};
DE EC=3.6.2.1 {ECO:0000269|PubMed:19896942};
DE AltName: Full=5'-adenylyl sulfate phosphorylase HINT4 {ECO:0000305|PubMed:19896942};
DE AltName: Full=Adenylylsulfate:phosphate adenylyltransferase HINT4 {ECO:0000305|PubMed:19896942};
DE AltName: Full=Histidine triad nucleotide-binding protein 4 {ECO:0000305};
GN Name=HINT4 {ECO:0000303|PubMed:19896942};
GN OrderedLocusNames=At4g16566 {ECO:0000312|Araport:AT4G16566};
GN ORFNames=dl4305c {ECO:0000312|EMBL:CAB10432.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=19329564; DOI=10.1104/pp.109.137703;
RA Reumann S., Quan S., Aung K., Yang P., Manandhar-Shrestha K., Holbrook D.,
RA Linka N., Switzenberg R., Wilkerson C.G., Weber A.P., Olsen L.J., Hu J.;
RT "In-depth proteome analysis of Arabidopsis leaf peroxisomes combined with
RT in vivo subcellular targeting verification indicates novel metabolic and
RT regulatory functions of peroxisomes.";
RL Plant Physiol. 150:125-143(2009).
RN [8]
RP FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, AND SUBUNIT.
RX PubMed=19896942; DOI=10.1016/j.febslet.2009.11.003;
RA Guranowski A., Wojdyla A.M., Zimny J., Wypijewska A., Kowalska J.,
RA Jemielity J., Davis R.E., Bieganowski P.;
RT "Dual activity of certain HIT-proteins: A. thaliana Hint4 and C. elegans
RT DcpS act on adenosine 5'-phosphosulfate as hydrolases (forming AMP) and as
RT phosphorylases (forming ADP).";
RL FEBS Lett. 584:93-98(2010).
CC -!- FUNCTION: Possesses adenylylsulfatase activity in vitro, releasing AMP
CC and sulfate from adenylyl sulfate. Possesses also adenosine 5'-
CC phosphosulfate (APS) phosphorylase activity in vitro. Catalyzes the
CC phosphorolysis of APS, leading to ADP and sulfate.
CC {ECO:0000269|PubMed:19896942}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ADP + H(+) + sulfate = adenosine 5'-phosphosulfate +
CC phosphate; Xref=Rhea:RHEA:16529, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16189, ChEBI:CHEBI:43474, ChEBI:CHEBI:58243,
CC ChEBI:CHEBI:456216; EC=2.7.7.5;
CC Evidence={ECO:0000269|PubMed:19896942};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=adenosine 5'-phosphosulfate + H2O = AMP + 2 H(+) + sulfate;
CC Xref=Rhea:RHEA:17041, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16189, ChEBI:CHEBI:58243, ChEBI:CHEBI:456215; EC=3.6.2.1;
CC Evidence={ECO:0000269|PubMed:19896942};
CC -!- ACTIVITY REGULATION: The adenosine 5'-phosphosulfate phosphorylase
CC activity is enhanced at low pH. {ECO:0000269|PubMed:19896942}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:19896942}.
CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000269|PubMed:19329564}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM66018.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB10432.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 3 genes: At4g16560, At4g16563 and At4g16566.; Evidence={ECO:0000305};
CC Sequence=CAB78698.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 3 genes: At4g16560, At4g16563 and At4g16566.; Evidence={ECO:0000305};
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DR EMBL; Z97341; CAB10432.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161544; CAB78698.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; ANM67558.1; -; Genomic_DNA.
DR EMBL; BT004792; AAO44058.1; -; mRNA.
DR EMBL; AK175206; BAD42969.1; -; mRNA.
DR EMBL; AK176221; BAD43984.1; -; mRNA.
DR EMBL; AY088482; AAM66018.1; ALT_SEQ; mRNA.
DR PIR; F71432; F71432.
DR RefSeq; NP_567507.1; NM_117757.4.
DR AlphaFoldDB; Q84VV6; -.
DR SMR; Q84VV6; -.
DR STRING; 3702.AT4G16566.1; -.
DR PaxDb; Q84VV6; -.
DR PRIDE; Q84VV6; -.
DR ProteomicsDB; 230194; -.
DR EnsemblPlants; AT4G16566.3; AT4G16566.3; AT4G16566.
DR GeneID; 827357; -.
DR Gramene; AT4G16566.3; AT4G16566.3; AT4G16566.
DR KEGG; ath:AT4G16566; -.
DR Araport; AT4G16566; -.
DR TAIR; locus:505006484; AT4G16566.
DR eggNOG; KOG1339; Eukaryota.
DR HOGENOM; CLU_056776_4_3_1; -.
DR InParanoid; Q84VV6; -.
DR OMA; CRIAGRQ; -.
DR BioCyc; MetaCyc:AT4G16566-MON; -.
DR BRENDA; 2.7.7.5; 399.
DR PRO; PR:Q84VV6; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q84VV6; baseline and differential.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005777; C:peroxisome; IDA:TAIR.
DR GO; GO:0047627; F:adenylylsulfatase activity; IDA:TAIR.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0004780; F:sulfate adenylyltransferase (ADP) activity; IDA:TAIR.
DR GO; GO:0009150; P:purine ribonucleotide metabolic process; IDA:TAIR.
DR GO; GO:0006790; P:sulfur compound metabolic process; IDA:TAIR.
DR Gene3D; 3.30.428.10; -; 1.
DR InterPro; IPR001310; Histidine_triad_HIT.
DR InterPro; IPR011146; HIT-like.
DR InterPro; IPR036265; HIT-like_sf.
DR PRINTS; PR00332; HISTRIAD.
DR SUPFAM; SSF54197; SSF54197; 1.
DR PROSITE; PS51084; HIT_2; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Nucleotide-binding; Nucleotidyltransferase; Peroxisome;
KW Reference proteome; Transferase.
FT CHAIN 1..146
FT /note="Bifunctional adenosine 5'-phosphosulfate
FT phosphorylase/adenylylsulfatase HINT4"
FT /id="PRO_0000436748"
FT DOMAIN 9..120
FT /note="HIT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00464"
FT MOTIF 101..105
FT /note="Histidine triad motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00464"
FT ACT_SITE 105
FT /note="Tele-AMP-histidine intermediate"
FT /evidence="ECO:0000250|UniProtKB:P49789"
SQ SEQUENCE 146 AA; 16798 MW; C6B22CC7FC7271D7 CRC64;
MAGVNQACIF CEIVRNPTTT RLLHTDEKVI AFQDIKPAAQ RHYLVIPKEH IPTVNDLQRR
DEDYSLVRHM LSVGQQLLQK DAPQSIHRFG FHQPPFNSVD HLHLHCFALP YVPRWKAIKY
KSLGPLGGFI EAETLLEKIR PLLSKV
