HIN_SALTY
ID HIN_SALTY Reviewed; 190 AA.
AC P03013;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=DNA-invertase hin;
GN Name=hin; OrderedLocusNames=STM2772;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6933466; DOI=10.1073/pnas.77.7.4196;
RA Zieg J., Simon M.;
RT "Analysis of the nucleotide sequence of an invertible controlling
RT element.";
RL Proc. Natl. Acad. Sci. U.S.A. 77:4196-4200(1980).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6271461; DOI=10.1101/sqb.1981.045.01.005;
RA Silverman M., Zieg J., Mandel G., Simon M.;
RT "Analysis of the functional components of the phase variation system.";
RL Cold Spring Harb. Symp. Quant. Biol. 45:17-26(1981).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6251543; DOI=10.1126/science.6251543;
RA Simon M., Zieg J., Silverman M., Mandel G., Doolittle R.;
RT "Phase variation: evolution of a controlling element.";
RL Science 209:1370-1374(1980).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 139-190.
RX PubMed=8278807; DOI=10.1126/science.8278807;
RA Feng J.-A., Johnson R.C., Dickerson R.E.;
RT "Hin recombinase bound to DNA: the origin of specificity in major and minor
RT groove interactions.";
RL Science 263:348-355(1994).
RN [6]
RP REVIEW.
RX PubMed=2166334; DOI=10.1126/science.2166334;
RA Heichman K.A., Johnson R.C.;
RT "The Hin invertasome: protein-mediated joining of distant recombination
RT sites at the enhancer.";
RL Science 249:511-517(1990).
CC -!- FUNCTION: A DNA fragment of approximately 900 base pairs, adjacent to
CC the fljB (H2) gene, which specifies the synthesis of phase-2 flagellin,
CC can exist in either orientation with respect to fljB. The orientation
CC of the inversion region controls expression of fljB. The hin gene
CC occupies about two-thirds of the inversion region; it is required for
CC the inversion of the fljB controlling region.
CC -!- SIMILARITY: Belongs to the site-specific recombinase resolvase family.
CC {ECO:0000305}.
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DR EMBL; J01801; AAA27073.1; -; Genomic_DNA.
DR EMBL; V01370; CAA24654.1; -; Genomic_DNA.
DR EMBL; M12292; AAA27140.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21658.1; -; Genomic_DNA.
DR PIR; A03544; JWEBT.
DR RefSeq; NP_461699.1; NC_003197.2.
DR RefSeq; WP_000190912.1; NC_003197.2.
DR PDB; 1HCR; X-ray; 2.30 A; A=139-190.
DR PDB; 1IJW; X-ray; 2.40 A; C=139-190.
DR PDB; 1JJ6; X-ray; 2.30 A; C=139-190.
DR PDB; 1JJ8; X-ray; 2.75 A; C=139-190.
DR PDB; 1JKO; X-ray; 2.24 A; C=139-190.
DR PDB; 1JKP; X-ray; 2.80 A; C=139-190.
DR PDB; 1JKQ; X-ray; 2.86 A; C=139-190.
DR PDB; 1JKR; X-ray; 2.28 A; C=139-190.
DR PDBsum; 1HCR; -.
DR PDBsum; 1IJW; -.
DR PDBsum; 1JJ6; -.
DR PDBsum; 1JJ8; -.
DR PDBsum; 1JKO; -.
DR PDBsum; 1JKP; -.
DR PDBsum; 1JKQ; -.
DR PDBsum; 1JKR; -.
DR AlphaFoldDB; P03013; -.
DR SMR; P03013; -.
DR STRING; 99287.STM2772; -.
DR PaxDb; P03013; -.
DR EnsemblBacteria; AAL21658; AAL21658; STM2772.
DR GeneID; 1254295; -.
DR KEGG; stm:STM2772; -.
DR PATRIC; fig|99287.12.peg.2924; -.
DR HOGENOM; CLU_010686_8_0_6; -.
DR OMA; INRHEQE; -.
DR PhylomeDB; P03013; -.
DR BioCyc; SENT99287:STM2772-MON; -.
DR EvolutionaryTrace; P03013; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000150; F:DNA strand exchange activity; IBA:GO_Central.
DR GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IBA:GO_Central.
DR Gene3D; 3.40.50.1390; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR006118; Recombinase_CS.
DR InterPro; IPR006119; Resolv_N.
DR InterPro; IPR036162; Resolvase-like_N_sf.
DR InterPro; IPR006120; Resolvase_HTH_dom.
DR Pfam; PF02796; HTH_7; 1.
DR Pfam; PF00239; Resolvase; 1.
DR SMART; SM00857; Resolvase; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF53041; SSF53041; 1.
DR PROSITE; PS00397; RECOMBINASES_1; 1.
DR PROSITE; PS00398; RECOMBINASES_2; 1.
DR PROSITE; PS51736; RECOMBINASES_3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA integration; DNA invertase; DNA recombination;
KW DNA-binding; Reference proteome.
FT CHAIN 1..190
FT /note="DNA-invertase hin"
FT /id="PRO_0000196359"
FT DOMAIN 2..135
FT /note="Resolvase/invertase-type recombinase catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT DNA_BIND 162..181
FT /note="H-T-H motif"
FT ACT_SITE 10
FT /note="O-(5'-phospho-DNA)-serine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT CONFLICT 184
FT /note="R -> S (in Ref. 1, 2 and 3)"
FT /evidence="ECO:0000305"
FT HELIX 148..157
FT /evidence="ECO:0007829|PDB:1JKO"
FT HELIX 162..167
FT /evidence="ECO:0007829|PDB:1JKO"
FT HELIX 173..179
FT /evidence="ECO:0007829|PDB:1JKO"
FT HELIX 182..184
FT /evidence="ECO:0007829|PDB:1JJ6"
SQ SEQUENCE 190 AA; 21426 MW; 3599962ADE1748E1 CRC64;
MATIGYIRVS TIDQNIDLQR NALTSANCDR IFEDRISGKI ANRPGLKRAL KYVNKGDTLV
VWKLDRLGRS VKNLVALISE LHERGAHFHS LTDSIDTSSA MGRFFFHVMS ALAEMERELI
VERTLAGLAA ARAQGRLGGR PRAINKHEQE QISRLLEKGH PRQQLAIIFG IGVSTLYRYF
PASRIKKRMN
