HIP20_ARATH
ID HIP20_ARATH Reviewed; 152 AA.
AC Q9C9A3;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Heavy metal-associated isoprenylated plant protein 20 {ECO:0000303|PubMed:21072340, ECO:0000303|PubMed:23368984};
DE Short=AtHIP20 {ECO:0000303|PubMed:23368984};
DE Short=AtHIPP20 {ECO:0000303|PubMed:21072340};
DE Flags: Precursor;
GN Name=HIPP20 {ECO:0000303|PubMed:21072340, ECO:0000303|PubMed:23368984};
GN OrderedLocusNames=At1g71050 {ECO:0000312|Araport:AT1G71050};
GN ORFNames=F23N20.4 {ECO:0000312|EMBL:AAG51694.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INTERACTION WITH ZHD11/HB29.
RC STRAIN=cv. Columbia;
RX PubMed=18974936; DOI=10.1007/s11103-008-9419-0;
RA Barth O., Vogt S., Uhlemann R., Zschiesche W., Humbeck K.;
RT "Stress induced and nuclear localized HIPP26 from Arabidopsis thaliana
RT interacts via its heavy metal associated domain with the drought stress
RT related zinc finger transcription factor ATHB29.";
RL Plant Mol. Biol. 69:213-226(2009).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, NOMENCLATURE, GENE FAMILY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=21072340; DOI=10.1039/c003484c;
RA Tehseen M., Cairns N., Sherson S., Cobbett C.S.;
RT "Metallochaperone-like genes in Arabidopsis thaliana.";
RL Metallomics 2:556-564(2010).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=23368984; DOI=10.1111/febs.12159;
RA de Abreu-Neto J.B., Turchetto-Zolet A.C., de Oliveira L.F., Zanettini M.H.,
RA Margis-Pinheiro M.;
RT "Heavy metal-associated isoprenylated plant protein (HIPP):
RT characterization of a family of proteins exclusive to plants.";
RL FEBS J. 280:1604-1616(2013).
CC -!- FUNCTION: Heavy-metal-binding protein. Binds cadmium. May be involved
CC in cadmium transport and play a role in cadmium detoxification.
CC {ECO:0000269|PubMed:21072340}.
CC -!- SUBUNIT: Interacts with ZHD11/HB29. {ECO:0000269|PubMed:18974936}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q9SZN7}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, shoot apical meristem, leaves
CC and flowers. {ECO:0000269|PubMed:21072340}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Hipp20, hipp21 and hipp22
CC triple mutants are cadmium sensitive. {ECO:0000269|PubMed:21072340}.
CC -!- SIMILARITY: Belongs to the HIPP family. {ECO:0000305}.
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DR EMBL; AC016972; AAG51694.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35156.1; -; Genomic_DNA.
DR EMBL; AK117880; BAC42520.1; -; mRNA.
DR EMBL; BT003666; AAO39894.1; -; mRNA.
DR EMBL; AY087875; AAM65427.1; -; mRNA.
DR PIR; H96734; H96734.
DR RefSeq; NP_177261.1; NM_105774.4.
DR AlphaFoldDB; Q9C9A3; -.
DR SMR; Q9C9A3; -.
DR IntAct; Q9C9A3; 8.
DR STRING; 3702.AT1G71050.1; -.
DR PaxDb; Q9C9A3; -.
DR PRIDE; Q9C9A3; -.
DR EnsemblPlants; AT1G71050.1; AT1G71050.1; AT1G71050.
DR GeneID; 843445; -.
DR Gramene; AT1G71050.1; AT1G71050.1; AT1G71050.
DR KEGG; ath:AT1G71050; -.
DR Araport; AT1G71050; -.
DR TAIR; locus:2026336; AT1G71050.
DR eggNOG; KOG1603; Eukaryota.
DR HOGENOM; CLU_100095_1_0_1; -.
DR InParanoid; Q9C9A3; -.
DR OMA; PYNMVAY; -.
DR OrthoDB; 1352064at2759; -.
DR PhylomeDB; Q9C9A3; -.
DR PRO; PR:Q9C9A3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C9A3; baseline and differential.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0055073; P:cadmium ion homeostasis; IMP:UniProtKB.
DR GO; GO:0071585; P:detoxification of cadmium ion; IMP:UniProtKB.
DR CDD; cd00371; HMA; 1.
DR InterPro; IPR045181; HIPP/ATX1-like.
DR InterPro; IPR006121; HMA_dom.
DR InterPro; IPR036163; HMA_dom_sf.
DR PANTHER; PTHR22814; PTHR22814; 1.
DR Pfam; PF00403; HMA; 1.
DR SUPFAM; SSF55008; SSF55008; 1.
DR PROSITE; PS50846; HMA_2; 1.
PE 1: Evidence at protein level;
KW Cadmium; Lipoprotein; Membrane; Metal-binding; Methylation; Prenylation;
KW Reference proteome.
FT CHAIN 1..149
FT /note="Heavy metal-associated isoprenylated plant protein
FT 20"
FT /id="PRO_0000435855"
FT PROPEP 150..152
FT /note="Removed in mature form"
FT /evidence="ECO:0000305"
FT /id="PRO_0000435856"
FT DOMAIN 27..90
FT /note="HMA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT BINDING 38
FT /ligand="Cd(2+)"
FT /ligand_id="ChEBI:CHEBI:48775"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280,
FT ECO:0000305|PubMed:21072340"
FT BINDING 41
FT /ligand="Cd(2+)"
FT /ligand_id="ChEBI:CHEBI:48775"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280,
FT ECO:0000305|PubMed:21072340"
FT MOD_RES 149
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250|UniProtKB:Q9SZN7"
FT LIPID 149
FT /note="S-farnesyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:Q9SZN7"
SQ SEQUENCE 152 AA; 17383 MW; AA804F713C490B68 CRC64;
MGALDSLSEY ISDYFRVTRK RRKRKVMQTV NIKVKMDCDG CERRVKNAVS SMKGVKSVEV
NRKIHKVTVS GYVEPKKVLK RIERTGKKAE IWPYVPYNMV AYPYAVGTYD KKAPAGYVRK
SEQSQLQLLP GAPENHYISL FSDENPNACT VM
