HIP21_ARATH
ID HIP21_ARATH Reviewed; 149 AA.
AC Q9LF57;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Heavy metal-associated isoprenylated plant protein 21 {ECO:0000303|PubMed:21072340, ECO:0000303|PubMed:23368984};
DE Short=AtHIP21 {ECO:0000303|PubMed:23368984};
DE Short=AtHIPP21 {ECO:0000303|PubMed:21072340, ECO:0000303|PubMed:23368984};
DE Flags: Precursor;
GN Name=HIPP21 {ECO:0000303|PubMed:21072340, ECO:0000303|PubMed:23368984};
GN OrderedLocusNames=At5g17450 {ECO:0000312|Araport:AT5G17450};
GN ORFNames=K3M16_20 {ECO:0000312|EMBL:CAC01889.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP INTERACTION WITH ZHD11/HB29.
RC STRAIN=cv. Columbia;
RX PubMed=18974936; DOI=10.1007/s11103-008-9419-0;
RA Barth O., Vogt S., Uhlemann R., Zschiesche W., Humbeck K.;
RT "Stress induced and nuclear localized HIPP26 from Arabidopsis thaliana
RT interacts via its heavy metal associated domain with the drought stress
RT related zinc finger transcription factor ATHB29.";
RL Plant Mol. Biol. 69:213-226(2009).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, NOMENCLATURE, GENE FAMILY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=21072340; DOI=10.1039/c003484c;
RA Tehseen M., Cairns N., Sherson S., Cobbett C.S.;
RT "Metallochaperone-like genes in Arabidopsis thaliana.";
RL Metallomics 2:556-564(2010).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=23368984; DOI=10.1111/febs.12159;
RA de Abreu-Neto J.B., Turchetto-Zolet A.C., de Oliveira L.F., Zanettini M.H.,
RA Margis-Pinheiro M.;
RT "Heavy metal-associated isoprenylated plant protein (HIPP):
RT characterization of a family of proteins exclusive to plants.";
RL FEBS J. 280:1604-1616(2013).
CC -!- FUNCTION: Heavy-metal-binding protein. Binds cadmium. May be involved
CC in cadmium transport and play a role in cadmium detoxification.
CC {ECO:0000269|PubMed:21072340}.
CC -!- SUBUNIT: Interacts with ZHD11/HB29. {ECO:0000269|PubMed:18974936}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q9SZN7}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences. {ECO:0000305};
CC Name=1;
CC IsoId=Q9LF57-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed at low levels in leaves and sepals.
CC {ECO:0000269|PubMed:21072340}.
CC -!- DISRUPTION PHENOTYPE: Hipp20, hipp21 and hipp22 triple mutants are
CC cadmium sensitive. {ECO:0000269|PubMed:21072340}.
CC -!- SIMILARITY: Belongs to the HIPP family. {ECO:0000305}.
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DR EMBL; AL391150; CAC01889.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92427.1; -; Genomic_DNA.
DR EMBL; BT029323; ABK32137.1; -; mRNA.
DR PIR; T51471; T51471.
DR RefSeq; NP_197247.1; NM_121751.3. [Q9LF57-1]
DR AlphaFoldDB; Q9LF57; -.
DR SMR; Q9LF57; -.
DR IntAct; Q9LF57; 4.
DR STRING; 3702.AT5G17450.1; -.
DR PaxDb; Q9LF57; -.
DR PRIDE; Q9LF57; -.
DR EnsemblPlants; AT5G17450.1; AT5G17450.1; AT5G17450. [Q9LF57-1]
DR GeneID; 831611; -.
DR Gramene; AT5G17450.1; AT5G17450.1; AT5G17450. [Q9LF57-1]
DR KEGG; ath:AT5G17450; -.
DR Araport; AT5G17450; -.
DR TAIR; locus:2157457; AT5G17450.
DR eggNOG; KOG1603; Eukaryota.
DR HOGENOM; CLU_100095_1_0_1; -.
DR InParanoid; Q9LF57; -.
DR OMA; TRTKHKP; -.
DR OrthoDB; 1352064at2759; -.
DR PhylomeDB; Q9LF57; -.
DR PRO; PR:Q9LF57; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LF57; baseline and differential.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0055073; P:cadmium ion homeostasis; IMP:UniProtKB.
DR GO; GO:0071585; P:detoxification of cadmium ion; IMP:UniProtKB.
DR CDD; cd00371; HMA; 1.
DR InterPro; IPR045181; HIPP/ATX1-like.
DR InterPro; IPR006121; HMA_dom.
DR InterPro; IPR036163; HMA_dom_sf.
DR PANTHER; PTHR22814; PTHR22814; 1.
DR Pfam; PF00403; HMA; 1.
DR SUPFAM; SSF55008; SSF55008; 1.
DR PROSITE; PS50846; HMA_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cadmium; Lipoprotein; Membrane; Metal-binding;
KW Methylation; Prenylation; Reference proteome.
FT CHAIN 1..146
FT /note="Heavy metal-associated isoprenylated plant protein
FT 21"
FT /id="PRO_0000435857"
FT PROPEP 147..149
FT /note="Removed in mature form"
FT /evidence="ECO:0000305"
FT /id="PRO_0000435858"
FT DOMAIN 25..88
FT /note="HMA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT BINDING 36
FT /ligand="a metal cation"
FT /ligand_id="ChEBI:CHEBI:25213"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT BINDING 39
FT /ligand="a metal cation"
FT /ligand_id="ChEBI:CHEBI:25213"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT MOD_RES 146
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250|UniProtKB:Q9SZN7"
FT LIPID 146
FT /note="S-farnesyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:Q9SZN7"
SQ SEQUENCE 149 AA; 16996 MW; 5F4AFB521FB8D6AC CRC64;
MGAFDYISSF CSYTYANAKT KRKPLQTVDI KVKMDCDGCE RRVRNVVRRM KGVKSVEVNR
KQSRITVNGH VDPNKVLKRV KSTGKKAEFW PYIPQHMVYY PFAPGMYDKR APAGHIRNPT
QSFPTANAPE ENYVSLFSDD NVHAACSIM
