HIP32_ARATH
ID HIP32_ARATH Reviewed; 473 AA.
AC Q9M8K5;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Heavy metal-associated isoprenylated plant protein 32 {ECO:0000303|PubMed:21072340, ECO:0000303|PubMed:23368984};
DE Short=AtHIP32 {ECO:0000303|PubMed:21072340, ECO:0000303|PubMed:23368984};
DE Flags: Precursor;
GN Name=HIPP32 {ECO:0000303|PubMed:21072340, ECO:0000303|PubMed:23368984};
GN OrderedLocusNames=At3g06130 {ECO:0000312|Araport:AT3G06130};
GN ORFNames=F28L1.7 {ECO:0000312|EMBL:AAF30306.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=21072340; DOI=10.1039/c003484c;
RA Tehseen M., Cairns N., Sherson S., Cobbett C.S.;
RT "Metallochaperone-like genes in Arabidopsis thaliana.";
RL Metallomics 2:556-564(2010).
RN [5]
RP GENE FAMILY, NOMENCLATURE, AND INDUCTION BY CADMIUM.
RX PubMed=23368984; DOI=10.1111/febs.12159;
RA de Abreu-Neto J.B., Turchetto-Zolet A.C., de Oliveira L.F., Zanettini M.H.,
RA Margis-Pinheiro M.;
RT "Heavy metal-associated isoprenylated plant protein (HIPP):
RT characterization of a family of proteins exclusive to plants.";
RL FEBS J. 280:1604-1616(2013).
CC -!- FUNCTION: Heavy-metal-binding protein. {ECO:0000250|UniProtKB:Q9LZF1}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences. {ECO:0000305};
CC Name=1;
CC IsoId=Q9M8K5-1; Sequence=Displayed;
CC -!- INDUCTION: Down-regulated by cadmium. {ECO:0000303|PubMed:23368984}.
CC -!- SIMILARITY: Belongs to the HIPP family. {ECO:0000305}.
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DR EMBL; AC018907; AAF30306.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74348.1; -; Genomic_DNA.
DR EMBL; AF326899; AAG41481.1; -; mRNA.
DR EMBL; AF349519; AAK15566.1; -; mRNA.
DR EMBL; AY045685; AAK74043.1; -; mRNA.
DR EMBL; AY149962; AAN31116.1; -; mRNA.
DR RefSeq; NP_566273.1; NM_111488.4. [Q9M8K5-1]
DR AlphaFoldDB; Q9M8K5; -.
DR SMR; Q9M8K5; -.
DR STRING; 3702.AT3G06130.1; -.
DR PaxDb; Q9M8K5; -.
DR PRIDE; Q9M8K5; -.
DR ProteomicsDB; 230192; -. [Q9M8K5-1]
DR EnsemblPlants; AT3G06130.1; AT3G06130.1; AT3G06130. [Q9M8K5-1]
DR GeneID; 819786; -.
DR Gramene; AT3G06130.1; AT3G06130.1; AT3G06130. [Q9M8K5-1]
DR KEGG; ath:AT3G06130; -.
DR Araport; AT3G06130; -.
DR TAIR; locus:2082425; AT3G06130.
DR eggNOG; KOG1603; Eukaryota.
DR HOGENOM; CLU_017291_1_0_1; -.
DR InParanoid; Q9M8K5; -.
DR OMA; DQYSHAF; -.
DR OrthoDB; 1397920at2759; -.
DR PRO; PR:Q9M8K5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M8K5; baseline and differential.
DR GO; GO:0009505; C:plant-type cell wall; HDA:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd00371; HMA; 1.
DR InterPro; IPR006121; HMA_dom.
DR InterPro; IPR036163; HMA_dom_sf.
DR Pfam; PF00403; HMA; 1.
DR SUPFAM; SSF55008; SSF55008; 1.
DR PROSITE; PS50846; HMA_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Lipoprotein; Metal-binding; Methylation; Prenylation;
KW Reference proteome.
FT CHAIN 1..470
FT /note="Heavy metal-associated isoprenylated plant protein
FT 32"
FT /id="PRO_0000437841"
FT PROPEP 471..473
FT /note="Removed in mature form"
FT /evidence="ECO:0000305"
FT /id="PRO_0000437842"
FT DOMAIN 9..72
FT /note="HMA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT REGION 96..139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 162..230
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 246..343
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 187..216
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 20
FT /ligand="a metal cation"
FT /ligand_id="ChEBI:CHEBI:25213"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT BINDING 23
FT /ligand="a metal cation"
FT /ligand_id="ChEBI:CHEBI:25213"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT MOD_RES 470
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250|UniProtKB:Q9SZN7"
FT LIPID 470
FT /note="S-farnesyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:Q9SZN7"
SQ SEQUENCE 473 AA; 49192 MW; 47F182C7CE63C988 CRC64;
MSKEEFMKIQ TCVLKVNIHC DGCKQKVKKI LQKIEGVFTT KIDSEQGKVT VSGSVDPSVL
IKKLAKSGKH AEIWGAPKGN NNPNQSQMAN QFKGMQIDHG KAGGGGGGNN NNNKKGQKNG
GGGGGGGGGG NSNAPKMGQQ LNPQHLQQLQ QLQKMKGFQD LKLPPQLKGS VPVNKNQNQK
GVKFDVPEDD DDDDFSDEFD DEFTDDDDDE FDDEFDDLPL PSNKMKPNMT MMPNAQQMMM
NAQKNANLAG GPAKNGGKGA PAAGGGGAGG GKGAGGGAKG GPGNQNQGGG KNGGGGHPQD
GKNGGGGGGP NAGKKGNGGG GPMAGGVSGG FRPMGGGGPQ NMSMPMGGQM GMGGPMGNMP
AVQGLPATGP GGAPQGYFQG AGIDPMQMQQ QQQQQQYLAA VMNQQRAMGN ERFQPMMYAR
PPPAVNYMPP NPHQYPNPHP YPYPYPYPYP PPYGNDQYSH AFSDENTSSC DIM
