ID   HIP_MARPU               Reviewed;          82 AA.
AC   P59860;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   26-SEP-2003, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=High-potential iron-sulfur protein;
DE            Short=HiPIP;
GN   Name=hip;
OS   Marichromatium purpuratum (Chromatium purpuratum).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae;
OC   Marichromatium.
OX   NCBI_TaxID=37487;
RN   [1]
RP   PROTEIN SEQUENCE OF 1-25, AND CHARACTERIZATION.
RX   PubMed=8672482; DOI=10.1021/bi952731v;
RA   Kerfeld C.A., Chan C., Hirasawa M., Kleis-SanFrancisco S., Yeates T.O.,
RA   Knaff D.B.;
RT   "Isolation and characterization of soluble electron transfer proteins from
RT   Chromatium purpuratum.";
RL   Biochemistry 35:7812-7818(1996).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR (4FE-4S).
RX   PubMed=9760225; DOI=10.1021/bi9810252;
RA   Kerfeld C.A., Salmeen A.E., Yeates T.O.;
RT   "Crystal structure and possible dimerization of the high-potential iron-
RT   sulfur protein from Chromatium purpuratum.";
RL   Biochemistry 37:13911-13917(1998).
CC   -!- FUNCTION: Specific class of high-redox-potential 4Fe-4S ferredoxins.
CC       Functions in anaerobic electron transport in most purple and in some
CC       other photosynthetic bacteria and in at least one genus (Paracoccus) of
CC       halophilic, denitrifying bacteria.
CC   -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Periplasm.
CC   -!- SIMILARITY: Belongs to the high-potential iron-sulfur protein (HiPIP)
CC       family. {ECO:0000255|PROSITE-ProRule:PRU00705}.
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DR   PDB; 3HIP; X-ray; 2.80 A; A/B/C=1-82.
DR   PDBsum; 3HIP; -.
DR   AlphaFoldDB; P59860; -.
DR   SMR; P59860; -.
DR   EvolutionaryTrace; P59860; -.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019646; P:aerobic electron transport chain; IEA:InterPro.
DR   Gene3D; 4.10.490.10; -; 1.
DR   InterPro; IPR000170; High_potential_FeS_prot.
DR   InterPro; IPR036369; HIPIP_sf.
DR   Pfam; PF01355; HIPIP; 1.
DR   SUPFAM; SSF57652; SSF57652; 1.
DR   PROSITE; PS51373; HIPIP; 1.
PE   1: Evidence at protein level;
KW   3D-structure; 4Fe-4S; Direct protein sequencing; Electron transport; Iron;
KW   Iron-sulfur; Metal-binding; Periplasm; Transport.
FT   CHAIN           1..82
FT                   /note="High-potential iron-sulfur protein"
FT                   /id="PRO_0000220422"
FT   BINDING         42
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000269|PubMed:9760225"
FT   BINDING         45
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000269|PubMed:9760225"
FT   BINDING         60
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000269|PubMed:9760225"
FT   BINDING         74
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000269|PubMed:9760225"
FT   HELIX           11..16
FT                   /evidence="ECO:0007829|PDB:3HIP"
FT   STRAND          19..21
FT                   /evidence="ECO:0007829|PDB:3HIP"
FT   HELIX           22..24
FT                   /evidence="ECO:0007829|PDB:3HIP"
FT   HELIX           27..30
FT                   /evidence="ECO:0007829|PDB:3HIP"
FT   STRAND          33..35
FT                   /evidence="ECO:0007829|PDB:3HIP"
FT   HELIX           37..39
FT                   /evidence="ECO:0007829|PDB:3HIP"
FT   HELIX           42..44
FT                   /evidence="ECO:0007829|PDB:3HIP"
FT   STRAND          48..54
FT                   /evidence="ECO:0007829|PDB:3HIP"
FT   STRAND          57..60
FT                   /evidence="ECO:0007829|PDB:3HIP"
FT   STRAND          67..69
FT                   /evidence="ECO:0007829|PDB:3HIP"
SQ   SEQUENCE   82 AA;  8753 MW;  2C875E9C8DB8E06D CRC64;
     VPANAVTESD PAAVALKYHR DAASSERVAA ARPGLPPEEQ HCENCQFMNP DSAAADWKGC
     QLFPGKLINL SGWCASWTLR AG