ID   HIR2_HIRME              Reviewed;          65 AA.
AC   P28504;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Hirudin-2;
DE   AltName: Full=Hirudin II;
OS   Hirudo medicinalis (Medicinal leech).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Clitellata;
OC   Hirudinea; Hirudinida; Hirudiniformes; Hirudinidae; Hirudo.
OX   NCBI_TaxID=6421;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=2792365; DOI=10.1016/0014-5793(89)81070-1;
RA   Scharf M., Engels J., Tripier D.;
RT   "Primary structures of new 'iso-hirudins'.";
RL   FEBS Lett. 255:105-110(1989).
CC   -!- FUNCTION: Hirudin is a potent thrombin-specific protease inhibitor. It
CC       forms a stable non-covalent complex with alpha-thrombin, thereby
CC       abolishing its ability to cleave fibrinogen.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I14 (hirudin) family.
CC       {ECO:0000305}.
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DR   PIR; S05678; S05678.
DR   PDB; 1A3B; X-ray; 1.80 A; I=49-64.
DR   PDB; 1A3E; X-ray; 1.85 A; I=49-64.
DR   PDB; 1ABI; X-ray; 2.30 A; I=54-64.
DR   PDB; 1C1U; X-ray; 1.75 A; I=55-65.
DR   PDB; 1C1V; X-ray; 1.98 A; I=55-65.
DR   PDB; 1C1W; X-ray; 1.90 A; I=55-65.
DR   PDB; 1C4U; X-ray; 2.10 A; 3=55-63.
DR   PDB; 1C5L; X-ray; 1.47 A; I=55-64.
DR   PDB; 1C5N; X-ray; 1.50 A; I=55-64.
DR   PDB; 1C5O; X-ray; 1.90 A; I=55-65.
DR   PDB; 1D9I; X-ray; 2.30 A; I=55-64.
DR   PDB; 1FPC; X-ray; 2.30 A; I=54-64.
DR   PDB; 1GHV; X-ray; 1.85 A; I=55-65.
DR   PDB; 1GHW; X-ray; 1.75 A; I=55-65.
DR   PDB; 1GHX; X-ray; 1.65 A; I=55-65.
DR   PDB; 1GHY; X-ray; 1.85 A; I=55-65.
DR   PDB; 1GJ4; X-ray; 1.81 A; I=55-65.
DR   PDB; 1GJ5; X-ray; 1.73 A; I=55-65.
DR   PDB; 1IHS; X-ray; 2.00 A; I=49-65.
DR   PDB; 1NM6; X-ray; 1.80 A; B=55-64.
DR   PDB; 1NT1; X-ray; 2.00 A; H=55-64.
DR   PDB; 1NY2; X-ray; 2.30 A; 3=55-64.
DR   PDB; 1O2G; X-ray; 1.58 A; I=55-65.
DR   PDB; 1QBV; X-ray; 1.80 A; E=55-65.
DR   PDB; 1SB1; X-ray; 1.90 A; I=55-63.
DR   PDB; 1SL3; X-ray; 1.81 A; B=55-64.
DR   PDB; 1TA2; X-ray; 2.30 A; B=55-64.
DR   PDB; 1TA6; X-ray; 1.90 A; B=55-64.
DR   PDB; 1TMT; X-ray; 2.20 A; J=53-65.
DR   PDB; 1TWX; X-ray; 2.40 A; C=55-64.
DR   PDB; 1VR1; X-ray; 1.90 A; I=55-64.
DR   PDB; 1XM1; X-ray; 2.30 A; H=55-64.
DR   PDB; 1YPE; X-ray; 1.81 A; I=55-64.
DR   PDB; 1YPG; X-ray; 1.80 A; I=55-64.
DR   PDB; 1YPJ; X-ray; 1.78 A; I=55-64.
DR   PDB; 1YPK; X-ray; 1.78 A; I=55-64.
DR   PDB; 1YPL; X-ray; 1.85 A; I=55-64.
DR   PDB; 1YPM; X-ray; 1.85 A; I=55-64.
DR   PDB; 1ZRB; X-ray; 1.90 A; B=55-65.
DR   PDB; 2GDE; X-ray; 2.00 A; D=55-64.
DR   PDB; 2HGT; X-ray; 2.20 A; J=54-64.
DR   PDB; 2PKS; X-ray; 2.50 A; D=55-64.
DR   PDB; 3VXE; X-ray; 1.25 A; J=54-64.
DR   PDB; 3VXF; Other; 1.60 A; J=54-64.
DR   PDB; 6ZUG; X-ray; 1.80 A; I=54-64.
DR   PDB; 6ZUH; X-ray; 1.70 A; I=54-64.
DR   PDB; 6ZUN; X-ray; 1.79 A; I=54-64.
DR   PDB; 6ZUU; X-ray; 1.94 A; I=54-64.
DR   PDB; 6ZUW; X-ray; 2.00 A; I=54-64.
DR   PDB; 6ZUX; X-ray; 1.94 A; I=54-64.
DR   PDB; 6ZV7; X-ray; 1.94 A; I=54-64.
DR   PDB; 6ZV8; X-ray; 1.70 A; I=54-64.
DR   PDBsum; 1A3B; -.
DR   PDBsum; 1A3E; -.
DR   PDBsum; 1ABI; -.
DR   PDBsum; 1C1U; -.
DR   PDBsum; 1C1V; -.
DR   PDBsum; 1C1W; -.
DR   PDBsum; 1C4U; -.
DR   PDBsum; 1C5L; -.
DR   PDBsum; 1C5N; -.
DR   PDBsum; 1C5O; -.
DR   PDBsum; 1D9I; -.
DR   PDBsum; 1FPC; -.
DR   PDBsum; 1GHV; -.
DR   PDBsum; 1GHW; -.
DR   PDBsum; 1GHX; -.
DR   PDBsum; 1GHY; -.
DR   PDBsum; 1GJ4; -.
DR   PDBsum; 1GJ5; -.
DR   PDBsum; 1IHS; -.
DR   PDBsum; 1NM6; -.
DR   PDBsum; 1NT1; -.
DR   PDBsum; 1NY2; -.
DR   PDBsum; 1O2G; -.
DR   PDBsum; 1QBV; -.
DR   PDBsum; 1SB1; -.
DR   PDBsum; 1SL3; -.
DR   PDBsum; 1TA2; -.
DR   PDBsum; 1TA6; -.
DR   PDBsum; 1TMT; -.
DR   PDBsum; 1TWX; -.
DR   PDBsum; 1VR1; -.
DR   PDBsum; 1XM1; -.
DR   PDBsum; 1YPE; -.
DR   PDBsum; 1YPG; -.
DR   PDBsum; 1YPJ; -.
DR   PDBsum; 1YPK; -.
DR   PDBsum; 1YPL; -.
DR   PDBsum; 1YPM; -.
DR   PDBsum; 1ZRB; -.
DR   PDBsum; 2GDE; -.
DR   PDBsum; 2HGT; -.
DR   PDBsum; 2PKS; -.
DR   PDBsum; 3VXE; -.
DR   PDBsum; 3VXF; -.
DR   PDBsum; 6ZUG; -.
DR   PDBsum; 6ZUH; -.
DR   PDBsum; 6ZUN; -.
DR   PDBsum; 6ZUU; -.
DR   PDBsum; 6ZUW; -.
DR   PDBsum; 6ZUX; -.
DR   PDBsum; 6ZV7; -.
DR   PDBsum; 6ZV8; -.
DR   AlphaFoldDB; P28504; -.
DR   SMR; P28504; -.
DR   Allergome; 9843; Hir me Hirudin.
DR   MEROPS; I14.001; -.
DR   EvolutionaryTrace; P28504; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.70.10.10; -; 1.
DR   InterPro; IPR024793; Hirudin.
DR   InterPro; IPR011061; Hirudin/antistatin.
DR   InterPro; IPR000429; Prot_inh_hirudin.
DR   Pfam; PF00713; Hirudin; 1.
DR   PIRSF; PIRSF001640; Hirudin; 1.
DR   PRINTS; PR00777; HIRUDIN.
DR   SUPFAM; SSF57262; SSF57262; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   Protease inhibitor; Secreted; Serine protease inhibitor; Sulfation.
FT   CHAIN           1..65
FT                   /note="Hirudin-2"
FT                   /id="PRO_0000195644"
FT   REGION          1..3
FT                   /note="Interaction with thrombin active site"
FT                   /evidence="ECO:0000250"
FT   REGION          39..65
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          55..65
FT                   /note="Interaction with fibrinogen-binding exosite of
FT                   thrombin"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         63
FT                   /note="Sulfotyrosine"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        45
FT                   /note="O-linked (GalNAc...) threonine"
FT                   /evidence="ECO:0000250"
FT   DISULFID        6..14
FT                   /evidence="ECO:0000250"
FT   DISULFID        16..28
FT                   /evidence="ECO:0000250"
FT   DISULFID        22..39
FT                   /evidence="ECO:0000250"
FT   HELIX           61..63
FT                   /evidence="ECO:0007829|PDB:1C5N"
SQ   SEQUENCE   65 AA;  6987 MW;  757EEAE55EAEB27E CRC64;
     ITYTDCTESG QDLCLCEGSN VCGKGNKCIL GSNGEENQCV TGEGTPKPQS HNDGDFEEIP
     EEYLQ