HIRA_CHICK
ID HIRA_CHICK Reviewed; 1019 AA.
AC P79987; Q8JIE6;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Protein HIRA;
DE Short=cHIRA;
DE AltName: Full=TUP1-like enhancer of split protein 1;
GN Name=HIRA; Synonyms=TUPLE1;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=9063744; DOI=10.1093/hmg/6.2.237;
RA Roberts C., Daw S.C., Halford S., Scambler P.J.;
RT "Cloning and developmental expression analysis of chick Hira (Chira), a
RT candidate gene for DiGeorge syndrome.";
RL Hum. Mol. Genet. 6:237-245(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], WD REPEATS, INTERACTION WITH HDAC1; HDAC2 AND
RP RBBP4, AND MUTAGENESIS OF 43-TRP-ASN-44; 121-TRP-ARG-122; 182-TRP-ASP-183;
RP 336-TRP-THR-337; LEU-993; LEU-996 AND LEU-997.
RC TISSUE=B-cell;
RX PubMed=15527972; DOI=10.1016/j.gene.2004.07.031;
RA Ahmad A., Takami Y., Nakayama T.;
RT "WD dipeptide motifs and LXXLL motif of chicken HIRA are essential for
RT interactions with the p48 subunit of chromatin assembly factor-1 and
RT histone deacetylase-2 in vitro and in vivo.";
RL Gene 342:125-136(2004).
RN [3]
RP INTERACTION WITH ASF1; HDAC1; HDAC2 AND RBBP4, AND MUTAGENESIS OF LEU-993;
RP LEU-996 AND LEU-997.
RX PubMed=14652010; DOI=10.1016/j.bbrc.2003.11.073;
RA Ahmad A., Takami Y., Nakayama T.;
RT "WD dipeptide motifs and LXXLL motif of chicken HIRA are necessary for
RT transcription repression and the latter motif is essential for interaction
RT with histone deacetylase-2 in vivo.";
RL Biochem. Biophys. Res. Commun. 312:1266-1272(2003).
RN [4]
RP FUNCTION.
RX PubMed=16024922; DOI=10.1074/jbc.m501426200;
RA Ahmad A., Kikuchi H., Takami Y., Nakayama T.;
RT "Different roles of N-terminal and C-terminal halves of HIRA in
RT transcription regulation of cell cycle-related genes that contribute to
RT control of vertebrate cell growth.";
RL J. Biol. Chem. 280:32090-32100(2005).
RN [5]
RP INTERACTION WITH ASF1.
RX PubMed=16537536; DOI=10.1074/jbc.m511590200;
RA Sanematsu F., Takami Y., Barman H.K., Fukagawa T., Ono T., Shibahara K.,
RA Nakayama T.;
RT "Asf1 is required for viability and chromatin assembly during DNA
RT replication in vertebrate cells.";
RL J. Biol. Chem. 281:13817-13827(2006).
CC -!- FUNCTION: Cooperates with ASF1A to promote replication-independent
CC chromatin assembly (By similarity). May regulate the transcription of a
CC variety of genes controlling cell growth. {ECO:0000250,
CC ECO:0000269|PubMed:16024922}.
CC -!- SUBUNIT: Interacts with ASF1, HDAC1, HDAC2 and RBBP4.
CC {ECO:0000269|PubMed:14652010, ECO:0000269|PubMed:15527972,
CC ECO:0000269|PubMed:16537536}.
CC -!- INTERACTION:
CC P79987; Q3C1E9: ASF1; NbExp=2; IntAct=EBI-996848, EBI-996834;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Expressed from gastrulation onwards with
CC predominant expression in the neuroepithelium during neurulation.
CC Expressed in regions of the embryo containing migrating neural crest
CC cells, particularly those emanating from rhombomeres 4 and 6. Expressed
CC throughout the rostral mesenchyme with higher levels of expression in
CC regions containing neural crest cells. At later stages strong
CC expression is seen in the neural crest derived regions of the head, the
CC branchial arches and the pharyngeal pouches. Expression at the
CC rhombomere boundaries increases around stage 16.
CC {ECO:0000269|PubMed:9063744}.
CC -!- SIMILARITY: Belongs to the WD repeat HIR1 family. {ECO:0000305}.
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DR EMBL; X99375; CAA67754.1; -; mRNA.
DR EMBL; AB091180; BAC11842.1; -; mRNA.
DR RefSeq; NP_989563.2; NM_204232.2.
DR AlphaFoldDB; P79987; -.
DR SMR; P79987; -.
DR BioGRID; 675113; 4.
DR IntAct; P79987; 1.
DR STRING; 9031.ENSGALP00000002393; -.
DR PaxDb; P79987; -.
DR GeneID; 374074; -.
DR KEGG; gga:374074; -.
DR CTD; 7290; -.
DR VEuPathDB; HostDB:geneid_374074; -.
DR eggNOG; KOG0973; Eukaryota.
DR InParanoid; P79987; -.
DR OrthoDB; 685536at2759; -.
DR PhylomeDB; P79987; -.
DR PRO; PR:P79987; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0000417; C:HIR complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0006336; P:DNA replication-independent chromatin assembly; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR031120; HIR1.
DR InterPro; IPR011494; Hira.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR13831; PTHR13831; 1.
DR Pfam; PF07569; Hira; 1.
DR Pfam; PF00400; WD40; 4.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Chromatin regulator; Nucleus; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation; WD repeat.
FT CHAIN 1..1019
FT /note="Protein HIRA"
FT /id="PRO_0000051018"
FT REPEAT 11..53
FT /note="WD 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:15527972"
FT REPEAT 68..107
FT /note="WD 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:15527972"
FT REPEAT 129..168
FT /note="WD 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:15527972"
FT REPEAT 172..211
FT /note="WD 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:15527972"
FT REPEAT 220..263
FT /note="WD 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:15527972"
FT REPEAT 266..322
FT /note="WD 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:15527972"
FT REPEAT 326..367
FT /note="WD 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00221,
FT ECO:0000269|PubMed:15527972"
FT REGION 23..443
FT /note="Interaction with RBBP4"
FT REGION 406..433
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 444..1019
FT /note="Interaction with HDAC1"
FT REGION 513..561
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 599..633
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 960..1019
FT /note="Interaction with HDAC2"
FT COMPBIAS 406..424
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 539..561
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 600..622
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 43..44
FT /note="WN->AA: Abrogates interaction with RBBP4."
FT /evidence="ECO:0000269|PubMed:15527972"
FT MUTAGEN 121..122
FT /note="WR->AA: Abrogates interaction with RBBP4."
FT /evidence="ECO:0000269|PubMed:15527972"
FT MUTAGEN 182..183
FT /note="WD->AA: Abrogates interaction with RBBP4."
FT /evidence="ECO:0000269|PubMed:15527972"
FT MUTAGEN 336..337
FT /note="WT->AA: Abrogates interaction with RBBP4 and impairs
FT transcriptional repression."
FT /evidence="ECO:0000269|PubMed:15527972"
FT MUTAGEN 993
FT /note="L->A: Abrogates interaction with HDAC2 and impairs
FT transcriptional repression."
FT /evidence="ECO:0000269|PubMed:14652010,
FT ECO:0000269|PubMed:15527972"
FT MUTAGEN 996
FT /note="L->A: Abrogates interaction with HDAC2."
FT /evidence="ECO:0000269|PubMed:14652010,
FT ECO:0000269|PubMed:15527972"
FT MUTAGEN 997
FT /note="L->A: Abrogates interaction with HDAC2."
FT /evidence="ECO:0000269|PubMed:14652010,
FT ECO:0000269|PubMed:15527972"
FT CONFLICT 124
FT /note="Missing (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 159
FT /note="N -> NA (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 206
FT /note="W -> G (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 230
FT /note="W -> G (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 315
FT /note="C -> S (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 329
FT /note="K -> E (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 356..357
FT /note="DF -> NS (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 367
FT /note="S -> T (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 371..376
FT /note="KSNIHQ -> NSPFYH (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 384
FT /note="A -> P (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 388
FT /note="E -> D (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 394..395
FT /note="TI -> IT (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 402..405
FT /note="LKYQ -> SQAIE (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 423..436
FT /note="ASGAATTAPKVASM -> MGTTTSVAGI (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 446
FT /note="R -> L (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 450
FT /note="L -> F (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 488
FT /note="I -> L (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 500..501
FT /note="QN -> HS (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 516
FT /note="L -> F (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 526
FT /note="T -> VA (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 530
FT /note="I -> V (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 538
FT /note="S -> N (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 541
FT /note="G -> S (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 585
FT /note="T -> S (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 604
FT /note="L -> F (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 607
FT /note="D -> E (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 626
FT /note="A -> V (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 672
FT /note="A -> V (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 679..680
FT /note="CI -> WL (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 729
FT /note="C -> G (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 753
FT /note="I -> K (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 763
FT /note="L -> Q (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 766..768
FT /note="FSA -> YSD (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 816
FT /note="D -> E (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 827
FT /note="D -> E (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 841..847
FT /note="PVMSMSD -> AVLALSE (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 853
FT /note="F -> S (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 893
FT /note="A -> T (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 976
FT /note="R -> T (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
FT CONFLICT 992
FT /note="L -> H (in Ref. 2; BAC11842)"
FT /evidence="ECO:0000305"
FT CONFLICT 1004
FT /note="L -> LR (in Ref. 1; CAA67754)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1019 AA; 112074 MW; 24DCECAFA52E9F60 CRC64;
MKLLKPTWVN HNGKPIFSVD IHPDGTKFAT GGQGQDSGKV VIWNMAPVLK EEDEKNENIP
KMLCQMDNHL ACVNCVRWSN NGVYLASGGD DKLIMVWKRA AYIGPSTVFG SSSKLTNVEQ
WRCVSILRSH SGDVMDVAWS PHDAWLASCS VDNTVVIWNA VKFPEILATL KGHSGLVKGL
TWDPVGKYIA SQADDRSLKV WRTMDWQLET SITKPFDECG GTTHVLRLSW SPDGHYLVSA
HAMNNSGPTA QIIERDGWKT NMDFVGHRKA VTVVKFNPKI FKKKQKNGSS TKSSCPYCCC
AVGSKDRSLS VWLTCLKRPL VVIHELFDKS IMDISWTLNG LGILVCSMDG SVAFLDFSQD
ELGDPLSEEE KSNIHQSTYG KSLAIMTEAQ LSTTIIENPE MLKYQQRQQQ QQAEQKNASI
REASGAATTA PKVASMVNGE SLEDIRKNLL KKQVETRTAD GRRRITPLCI AQLDTGDFST
AFFNSIPISG TLAGSIMSSQ NNQQLMSLDS NAANSLNTSK PSAEPTAASI KPTDDAASKD
GVNATSVSTA PPASSSSVLT TPSKIEPMKA FDSRFTERSK ATSGTAVVTN TNQTVVDRLK
DQNLIKDNKP KDILESSSDS EEKIPAAKPL SAPKRKLELE GETVEKKKKG RPRKDSRLVP
VTLTVQSPAA LASEKDAACI SAPALALKLP TPIPQKSFTL QVSSDPSMYL EVENEMTTVG
GSKLSRLKCN REGKEWETVL TSRILTAAGS CEIVCVACEK RMLSVFSACG RRLLPPIILN
TPISTLHCTG SYIMTLTTAA TLSVWDVHKQ TVIVRDESLQ TILSGSDTTV SQILLTQHGI
PVMSMSDGKA YCFNPSLSTW NLVSDKQDSL AQCADFRSSL PSQEAMLCSG PLAVIQGRTS
NSGRQAARLF SMPHLVQQET TLAYLENQIA AALMLQSSHE YRHWLLIYAR YLVNEGFEYR
LRELCKDLLG PVHYSRGSQW ESTVMGLRKR ELLKELLPVI GQNLFQRLFT EYQEQLDIL
