HIRV2_HIRME
ID HIRV2_HIRME Reviewed; 72 AA.
AC P09945;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 25-MAY-2022, entry version 132.
DE RecName: Full=Hirudin variant-2;
DE Flags: Precursor; Fragment;
OS Hirudo medicinalis (Medicinal leech).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Clitellata;
OC Hirudinea; Hirudinida; Hirudiniformes; Hirudinidae; Hirudo.
OX NCBI_TaxID=6421;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND SULFATION AT TYR-70.
RX PubMed=3513162; DOI=10.1073/pnas.83.4.1084;
RA Harvey R.P., Degryse E., Stefani L., Schamber F., Cazenave J.-P.,
RA Courtney M., Tolstoshev P., Lecocq J.-P.;
RT "Cloning and expression of a cDNA coding for the anticoagulant hirudin from
RT the bloodsucking leech, Hirudo medicinalis.";
RL Proc. Natl. Acad. Sci. U.S.A. 83:1084-1088(1986).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 8-72 OF MUTANT LYS-54 IN COMPLEX
RP WITH THROMBIN, AND DISULFIDE BONDS.
RX PubMed=2374926; DOI=10.1126/science.2374926;
RA Rydel T.J., Ravichandran K.G., Tulinsky A., Bode W., Huber R., Roitsch C.,
RA Fenton J.W. II;
RT "The structure of a complex of recombinant hirudin and human alpha-
RT thrombin.";
RL Science 249:277-280(1990).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 8-72 IN COMPLEX WITH THROMBIN.
RX PubMed=1920434; DOI=10.1016/0022-2836(91)80074-5;
RA Rydel T.J., Tulinsky A., Bode W., Huber R.;
RT "Refined structure of the hirudin-thrombin complex.";
RL J. Mol. Biol. 221:583-601(1991).
CC -!- FUNCTION: Hirudin is a potent thrombin-specific protease inhibitor. It
CC forms a stable non-covalent complex with alpha-thrombin, thereby
CC abolishing its ability to cleave fibrinogen.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the protease inhibitor I14 (hirudin) family.
CC {ECO:0000305}.
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DR EMBL; M12693; AAA29195.1; -; mRNA.
DR PIR; A37417; A37417.
DR PDB; 1A2C; X-ray; 2.10 A; I=60-71.
DR PDB; 1A46; X-ray; 2.12 A; I=60-71.
DR PDB; 1A4W; X-ray; 1.80 A; I=60-71.
DR PDB; 1A5G; X-ray; 2.06 A; I=60-71.
DR PDB; 1A61; X-ray; 2.20 A; I=60-71.
DR PDB; 1B5G; X-ray; 2.07 A; I=60-71.
DR PDB; 1DOJ; X-ray; 1.70 A; B=62-72.
DR PDB; 1K21; X-ray; 1.86 A; I=60-71.
DR PDB; 1K22; X-ray; 1.93 A; I=60-71.
DR PDB; 1NRS; X-ray; 2.40 A; I=60-71.
DR PDB; 1TMB; X-ray; 2.30 A; I=60-71.
DR PDB; 1TMU; X-ray; 2.50 A; J=62-72.
DR PDB; 1VZQ; X-ray; 1.54 A; I=62-72.
DR PDB; 1W7G; X-ray; 1.65 A; I=60-71.
DR PDB; 1WAY; X-ray; 2.02 A; I=62-71.
DR PDB; 2BVR; X-ray; 1.25 A; I=61-70.
DR PDB; 2BVS; X-ray; 1.40 A; I=61-71.
DR PDB; 2BVX; X-ray; 3.20 A; I=61-71.
DR PDB; 2BXT; X-ray; 1.83 A; I=61-71.
DR PDB; 2BXU; X-ray; 2.80 A; I=61-71.
DR PDB; 2C8W; X-ray; 1.96 A; I=61-72.
DR PDB; 2C8X; X-ray; 2.17 A; I=61-72.
DR PDB; 2C8Y; X-ray; 2.20 A; I=61-72.
DR PDB; 2C8Z; X-ray; 2.14 A; I=61-72.
DR PDB; 2C90; X-ray; 2.25 A; I=61-72.
DR PDB; 2C93; X-ray; 2.20 A; I=61-72.
DR PDB; 2R2M; X-ray; 2.10 A; H=62-72.
DR PDB; 2ZFQ; X-ray; 1.80 A; I=60-71.
DR PDB; 2ZFR; X-ray; 1.85 A; I=60-71.
DR PDB; 2ZG0; X-ray; 1.75 A; I=60-71.
DR PDB; 2ZHE; X-ray; 2.10 A; I=60-71.
DR PDB; 2ZHF; X-ray; 1.98 A; I=60-71.
DR PDB; 2ZHW; X-ray; 2.02 A; I=60-71.
DR PDB; 3BIU; X-ray; 2.30 A; I=60-71.
DR PDB; 3BIV; X-ray; 1.80 A; I=60-71.
DR PDB; 3F68; X-ray; 1.75 A; I=61-71.
DR PDB; 3HAT; X-ray; 2.50 A; I=60-71.
DR PDB; 3HTC; X-ray; 2.30 A; I=8-72.
DR PDB; 3P17; X-ray; 1.43 A; I=60-71.
DR PDB; 3QTO; X-ray; 1.52 A; I=60-72.
DR PDB; 3QTV; X-ray; 1.63 A; I=60-72.
DR PDB; 3QWC; X-ray; 1.75 A; I=60-72.
DR PDB; 3QX5; X-ray; 1.35 A; I=60-72.
DR PDB; 3RLW; X-ray; 1.69 A; I=60-72.
DR PDB; 3RLY; X-ray; 1.51 A; I=60-72.
DR PDB; 3RM0; X-ray; 1.34 A; I=60-72.
DR PDB; 3RM2; X-ray; 1.23 A; I=60-72.
DR PDB; 3RML; X-ray; 1.53 A; I=60-72.
DR PDB; 3RMM; X-ray; 1.58 A; I=60-72.
DR PDB; 3RMN; X-ray; 1.78 A; I=60-72.
DR PDB; 3RMO; X-ray; 1.40 A; I=60-72.
DR PDB; 3SHA; X-ray; 1.52 A; I=60-72.
DR PDB; 3SHC; X-ray; 1.90 A; I=60-72.
DR PDB; 3SI3; X-ray; 1.55 A; I=60-72.
DR PDB; 3SI4; X-ray; 1.27 A; I=60-72.
DR PDB; 3SV2; X-ray; 1.30 A; I=60-72.
DR PDB; 3T5F; X-ray; 1.45 A; I=60-72.
DR PDB; 3TU7; X-ray; 2.49 A; I=61-72.
DR PDB; 3U98; X-ray; 1.45 A; I=60-72.
DR PDB; 3U9A; X-ray; 1.58 A; I=60-72.
DR PDB; 3UWJ; X-ray; 1.50 A; I=60-72.
DR PDB; 4BAO; X-ray; 1.87 A; D=62-71.
DR PDB; 4E7R; X-ray; 2.25 A; I/J=62-72.
DR PDB; 4HTC; X-ray; 2.30 A; I=8-72.
DR PDB; 4LOY; X-ray; 1.77 A; I=62-71.
DR PDB; 4UD9; X-ray; 1.12 A; I=61-72.
DR PDB; 4UDW; X-ray; 1.16 A; I=62-72.
DR PDB; 4UE7; X-ray; 1.13 A; I=61-72.
DR PDB; 4UEH; X-ray; 1.16 A; I=61-72.
DR PDB; 4UFD; X-ray; 1.43 A; I=61-72.
DR PDB; 4UFE; X-ray; 1.59 A; I=61-72.
DR PDB; 4UFF; X-ray; 1.55 A; I=61-72.
DR PDB; 4UFG; X-ray; 1.65 A; I=61-72.
DR PDB; 5A2M; X-ray; 1.40 A; I=61-72.
DR PDB; 5AF9; X-ray; 1.18 A; I=61-72.
DR PDB; 5JFD; X-ray; 1.46 A; I=61-72.
DR PDB; 5JZY; X-ray; 1.27 A; I=61-72.
DR PDB; 5LCE; X-ray; 1.39 A; I=61-72.
DR PDB; 5LPD; X-ray; 1.50 A; I=61-72.
DR PDB; 5MJT; X-ray; 1.40 A; D=61-72.
DR PDB; 5MLS; X-ray; 1.62 A; D=61-72.
DR PDB; 5MM6; X-ray; 1.29 A; I=61-72.
DR PDB; 6EO8; X-ray; 1.94 A; I=60-71.
DR PDB; 6EO9; X-ray; 1.84 A; I=60-71.
DR PDB; 6GBW; X-ray; 1.45 A; I=61-72.
DR PDB; 6HSX; X-ray; 1.56 A; I=62-72.
DR PDB; 6I51; X-ray; 1.40 A; I=61-72.
DR PDB; 6ROT; X-ray; 1.34 A; A=61-72.
DR PDB; 6T3M; X-ray; 1.38 A; I=62-72.
DR PDB; 6T3Q; X-ray; 1.33 A; I=62-72.
DR PDB; 6T4A; X-ray; 1.31 A; I=62-72.
DR PDB; 6T52; X-ray; 1.45 A; I=62-72.
DR PDB; 6T53; X-ray; 1.35 A; I=62-72.
DR PDB; 6T54; X-ray; 1.57 A; I=62-72.
DR PDB; 6T55; X-ray; 1.39 A; I=62-72.
DR PDB; 6T56; X-ray; 1.31 A; I=62-72.
DR PDB; 6T57; X-ray; 1.57 A; I=62-72.
DR PDB; 6T89; X-ray; 2.00 A; I=61-72.
DR PDB; 6T8A; X-ray; 1.62 A; I=62-72.
DR PDB; 6TDT; X-ray; 1.53 A; I=62-72.
DR PDB; 6Y02; X-ray; 1.48 A; C=61-72.
DR PDB; 6Y9H; X-ray; 1.48 A; I=61-72.
DR PDB; 6YB6; X-ray; 1.33 A; I=61-72.
DR PDB; 6YHG; X-ray; 1.33 A; I=61-72.
DR PDB; 6YHJ; X-ray; 1.44 A; I=61-72.
DR PDB; 6YMP; X-ray; 1.42 A; I=61-72.
DR PDB; 6YN3; X-ray; 1.49 A; I=61-72.
DR PDB; 6YQV; X-ray; 1.45 A; I=61-72.
DR PDB; 6YSJ; X-ray; 1.45 A; I=61-72.
DR PDB; 6YSX; X-ray; 1.48 A; I=61-72.
DR PDB; 6ZGO; X-ray; 1.79 A; I=61-72.
DR PDB; 7AC9; X-ray; 1.39 A; I=61-72.
DR PDBsum; 1A2C; -.
DR PDBsum; 1A46; -.
DR PDBsum; 1A4W; -.
DR PDBsum; 1A5G; -.
DR PDBsum; 1A61; -.
DR PDBsum; 1B5G; -.
DR PDBsum; 1DOJ; -.
DR PDBsum; 1K21; -.
DR PDBsum; 1K22; -.
DR PDBsum; 1NRS; -.
DR PDBsum; 1TMB; -.
DR PDBsum; 1TMU; -.
DR PDBsum; 1VZQ; -.
DR PDBsum; 1W7G; -.
DR PDBsum; 1WAY; -.
DR PDBsum; 2BVR; -.
DR PDBsum; 2BVS; -.
DR PDBsum; 2BVX; -.
DR PDBsum; 2BXT; -.
DR PDBsum; 2BXU; -.
DR PDBsum; 2C8W; -.
DR PDBsum; 2C8X; -.
DR PDBsum; 2C8Y; -.
DR PDBsum; 2C8Z; -.
DR PDBsum; 2C90; -.
DR PDBsum; 2C93; -.
DR PDBsum; 2R2M; -.
DR PDBsum; 2ZFQ; -.
DR PDBsum; 2ZFR; -.
DR PDBsum; 2ZG0; -.
DR PDBsum; 2ZHE; -.
DR PDBsum; 2ZHF; -.
DR PDBsum; 2ZHW; -.
DR PDBsum; 3BIU; -.
DR PDBsum; 3BIV; -.
DR PDBsum; 3F68; -.
DR PDBsum; 3HAT; -.
DR PDBsum; 3HTC; -.
DR PDBsum; 3P17; -.
DR PDBsum; 3QTO; -.
DR PDBsum; 3QTV; -.
DR PDBsum; 3QWC; -.
DR PDBsum; 3QX5; -.
DR PDBsum; 3RLW; -.
DR PDBsum; 3RLY; -.
DR PDBsum; 3RM0; -.
DR PDBsum; 3RM2; -.
DR PDBsum; 3RML; -.
DR PDBsum; 3RMM; -.
DR PDBsum; 3RMN; -.
DR PDBsum; 3RMO; -.
DR PDBsum; 3SHA; -.
DR PDBsum; 3SHC; -.
DR PDBsum; 3SI3; -.
DR PDBsum; 3SI4; -.
DR PDBsum; 3SV2; -.
DR PDBsum; 3T5F; -.
DR PDBsum; 3TU7; -.
DR PDBsum; 3U98; -.
DR PDBsum; 3U9A; -.
DR PDBsum; 3UWJ; -.
DR PDBsum; 4BAO; -.
DR PDBsum; 4E7R; -.
DR PDBsum; 4HTC; -.
DR PDBsum; 4LOY; -.
DR PDBsum; 4UD9; -.
DR PDBsum; 4UDW; -.
DR PDBsum; 4UE7; -.
DR PDBsum; 4UEH; -.
DR PDBsum; 4UFD; -.
DR PDBsum; 4UFE; -.
DR PDBsum; 4UFF; -.
DR PDBsum; 4UFG; -.
DR PDBsum; 5A2M; -.
DR PDBsum; 5AF9; -.
DR PDBsum; 5JFD; -.
DR PDBsum; 5JZY; -.
DR PDBsum; 5LCE; -.
DR PDBsum; 5LPD; -.
DR PDBsum; 5MJT; -.
DR PDBsum; 5MLS; -.
DR PDBsum; 5MM6; -.
DR PDBsum; 6EO8; -.
DR PDBsum; 6EO9; -.
DR PDBsum; 6GBW; -.
DR PDBsum; 6HSX; -.
DR PDBsum; 6I51; -.
DR PDBsum; 6ROT; -.
DR PDBsum; 6T3M; -.
DR PDBsum; 6T3Q; -.
DR PDBsum; 6T4A; -.
DR PDBsum; 6T52; -.
DR PDBsum; 6T53; -.
DR PDBsum; 6T54; -.
DR PDBsum; 6T55; -.
DR PDBsum; 6T56; -.
DR PDBsum; 6T57; -.
DR PDBsum; 6T89; -.
DR PDBsum; 6T8A; -.
DR PDBsum; 6TDT; -.
DR PDBsum; 6Y02; -.
DR PDBsum; 6Y9H; -.
DR PDBsum; 6YB6; -.
DR PDBsum; 6YHG; -.
DR PDBsum; 6YHJ; -.
DR PDBsum; 6YMP; -.
DR PDBsum; 6YN3; -.
DR PDBsum; 6YQV; -.
DR PDBsum; 6YSJ; -.
DR PDBsum; 6YSX; -.
DR PDBsum; 6ZGO; -.
DR PDBsum; 7AC9; -.
DR AlphaFoldDB; P09945; -.
DR SMR; P09945; -.
DR Allergome; 9843; Hir me Hirudin.
DR MEROPS; I14.001; -.
DR EvolutionaryTrace; P09945; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR Gene3D; 2.70.10.10; -; 1.
DR InterPro; IPR024793; Hirudin.
DR InterPro; IPR011061; Hirudin/antistatin.
DR InterPro; IPR000429; Prot_inh_hirudin.
DR Pfam; PF00713; Hirudin; 1.
DR PIRSF; PIRSF001640; Hirudin; 1.
DR PRINTS; PR00777; HIRUDIN.
DR SUPFAM; SSF57262; SSF57262; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Glycoprotein; Protease inhibitor; Secreted;
KW Serine protease inhibitor; Signal; Sulfation.
FT SIGNAL <1..7
FT CHAIN 8..72
FT /note="Hirudin variant-2"
FT /id="PRO_0000013441"
FT REGION 8..10
FT /note="Interaction with thrombin active site"
FT REGION 47..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 62..72
FT /note="Interaction with fibrinogen-binding exosite of
FT thrombin"
FT MOD_RES 70
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000269|PubMed:3513162"
FT CARBOHYD 52
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000250"
FT DISULFID 13..21
FT /evidence="ECO:0000269|PubMed:2374926"
FT DISULFID 23..35
FT /evidence="ECO:0000269|PubMed:2374926"
FT DISULFID 29..46
FT /evidence="ECO:0000269|PubMed:2374926"
FT NON_TER 1
FT STRAND 18..22
FT /evidence="ECO:0007829|PDB:4HTC"
FT STRAND 24..26
FT /evidence="ECO:0007829|PDB:4HTC"
FT STRAND 33..36
FT /evidence="ECO:0007829|PDB:4HTC"
FT STRAND 45..49
FT /evidence="ECO:0007829|PDB:4HTC"
FT HELIX 68..70
FT /evidence="ECO:0007829|PDB:4UE7"
SQ SEQUENCE 72 AA; 7571 MW; C6EF2F28E9C45A5B CRC64;
AICVSQAITY TDCTESGQNL CLCEGSNVCG KGNKCILGSN GKGNQCVTGE GTPNPESHNN
GDFEEIPEEY LQ
