ID   3SO3_BUNCA              Reviewed;          65 AA.
AC   P83346;
DT   25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Bucain;
OS   Bungarus candidus (Malayan krait).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Bungarinae; Bungarus.
OX   NCBI_TaxID=92438;
RN   [1]
RP   PROTEIN SEQUENCE, X-RAY CRYSTALLOGRAPHY (1.61 ANGSTROMS), DISULFIDE BONDS,
RP   MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=12351845; DOI=10.1107/s0907444902011022;
RA   Watanabe L., Nirthanan S., Rajaseger G., Polikarpov I., Kini R.M.,
RA   Arni R.K.;
RT   "Crystallization and preliminary X-ray analysis of bucain, a novel toxin
RT   from the Malayan krait Bungarus candidus.";
RL   Acta Crystallogr. D 58:1879-1881(2002).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12351845}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=7207.4; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:12351845};
CC   -!- MISCELLANEOUS: Is classified as a P-type cytotoxin, since a proline
CC       residue stands at position 33 (Pro-31 in standard classification).
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Orphan group III sub-subfamily. {ECO:0000305}.
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DR   PDB; 1VYC; NMR; -; A=1-65.
DR   PDB; 2H8U; X-ray; 2.10 A; A/B=1-65.
DR   PDBsum; 1VYC; -.
DR   PDBsum; 2H8U; -.
DR   AlphaFoldDB; P83346; -.
DR   SMR; P83346; -.
DR   TCDB; 1.C.74.1.5; the snake cytotoxin (sct) family.
DR   EvolutionaryTrace; P83346; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   InterPro; IPR035076; Toxin/TOLIP.
DR   Pfam; PF00087; Toxin_TOLIP; 1.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Secreted; Toxin.
FT   CHAIN           1..65
FT                   /note="Bucain"
FT                   /evidence="ECO:0000269|PubMed:12351845"
FT                   /id="PRO_0000093535"
FT   DISULFID        3..24
FT                   /evidence="ECO:0000269|PubMed:12351845,
FT                   ECO:0000312|PDB:1VYC, ECO:0000312|PDB:2H8U"
FT   DISULFID        17..42
FT                   /evidence="ECO:0000269|PubMed:12351845,
FT                   ECO:0000312|PDB:1VYC, ECO:0000312|PDB:2H8U"
FT   DISULFID        46..57
FT                   /evidence="ECO:0000269|PubMed:12351845,
FT                   ECO:0000312|PDB:1VYC, ECO:0000312|PDB:2H8U"
FT   DISULFID        58..63
FT                   /evidence="ECO:0000269|PubMed:12351845,
FT                   ECO:0000312|PDB:1VYC, ECO:0000312|PDB:2H8U"
FT   STRAND          2..7
FT                   /evidence="ECO:0007829|PDB:2H8U"
FT   STRAND          12..16
FT                   /evidence="ECO:0007829|PDB:2H8U"
FT   STRAND          23..31
FT                   /evidence="ECO:0007829|PDB:2H8U"
FT   STRAND          39..45
FT                   /evidence="ECO:0007829|PDB:2H8U"
FT   STRAND          53..58
FT                   /evidence="ECO:0007829|PDB:2H8U"
SQ   SEQUENCE   65 AA;  7216 MW;  8F6E2FE325E44CF1 CRC64;
     RKCLIKYSQA NESSKTCPSG QLLCLKKWEI GNPSGKEVKR GCVATCPKPW KNEIIQCCAK
     DKCNA