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ANO6_MOUSE
ID   ANO6_MOUSE              Reviewed;         911 AA.
AC   Q6P9J9; Q8C242;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=Anoctamin-6;
DE   AltName: Full=Small-conductance calcium-activated nonselective cation channel;
DE            Short=SCAN channel;
DE   AltName: Full=Transmembrane protein 16F;
GN   Name=Ano6;
GN   Synonyms=Tmem16f {ECO:0000303|PubMed:21107324,
GN   ECO:0000303|PubMed:30785399};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=C57BL/6J; TISSUE=Dendritic cell;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=C57BL/6J; TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=18729231; DOI=10.1002/dvdy.21676;
RA   Rock J.R., Harfe B.D.;
RT   "Expression of TMEM16 paralogs during murine embryogenesis.";
RL   Dev. Dyn. 237:2566-2574(2008).
RN   [4]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-494; ASN-785 AND ASN-803.
RC   TISSUE=Myoblast;
RX   PubMed=19656770; DOI=10.1074/mcp.m900195-mcp200;
RA   Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D.,
RA   Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.;
RT   "The mouse C2C12 myoblast cell surface N-linked glycoproteome:
RT   identification, glycosite occupancy, and membrane orientation.";
RL   Mol. Cell. Proteomics 8:2555-2569(2009).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brown adipose tissue, Kidney, Lung, Pancreas, and Spleen;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [6]
RP   TISSUE SPECIFICITY.
RX   PubMed=20056604; DOI=10.1074/jbc.m109.065367;
RA   Schreiber R., Uliyakina I., Kongsuphol P., Warth R., Mirza M.,
RA   Martins J.R., Kunzelmann K.;
RT   "Expression and function of epithelial anoctamins.";
RL   J. Biol. Chem. 285:7838-7845(2010).
RN   [7]
RP   FUNCTION.
RX   PubMed=21107324; DOI=10.1038/nature09583;
RA   Suzuki J., Umeda M., Sims P.J., Nagata S.;
RT   "Calcium-dependent phospholipid scrambling by TMEM16F.";
RL   Nature 468:834-838(2010).
RN   [8]
RP   ABSENCE OF CALCIUM-ACTIVATED CHLORIDE CHANNEL ACTIVITY.
RX   PubMed=22075693; DOI=10.1152/ajpcell.00140.2011;
RA   Duran C., Qu Z., Osunkoya A.O., Cui Y., Hartzell H.C.;
RT   "ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are intracellular
RT   proteins.";
RL   Am. J. Physiol. 302:C482-C493(2012).
RN   [9]
RP   FUNCTION, DISRUPTION PHENOTYPE, ACTIVITY REGULATION, AND MUTAGENESIS OF
RP   ASP-409; GLN-559 AND GLU-667.
RX   PubMed=23021219; DOI=10.1016/j.cell.2012.07.036;
RA   Yang H., Kim A., David T., Palmer D., Jin T., Tien J., Huang F., Cheng T.,
RA   Coughlin S.R., Jan Y.N., Jan L.Y.;
RT   "TMEM16F forms a Ca(2+)-activated cation channel required for lipid
RT   scrambling in platelets during blood coagulation.";
RL   Cell 151:111-122(2012).
RN   [10]
RP   REVIEW.
RX   PubMed=22302790; DOI=10.1113/expphysiol.2011.058214;
RA   Winpenny J.P., Gray M.A.;
RT   "The anoctamin (TMEM16) gene family: calcium-activated chloride channels
RT   come of age.";
RL   Exp. Physiol. 97:175-176(2012).
RN   [11]
RP   REVIEW, AND FUNCTION.
RX   PubMed=21908539; DOI=10.1113/expphysiol.2011.058206;
RA   Kunzelmann K., Schreiber R., Kmit A., Jantarajit W., Martins J.R.,
RA   Faria D., Kongsuphol P., Ousingsawat J., Tian Y.;
RT   "Expression and function of epithelial anoctamins.";
RL   Exp. Physiol. 97:184-192(2012).
RN   [12]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=23532839; DOI=10.1074/jbc.m113.457937;
RA   Suzuki J., Fujii T., Imao T., Ishihara K., Kuba H., Nagata S.;
RT   "Calcium-dependent phospholipid scramblase activity of TMEM16 protein
RT   family members.";
RL   J. Biol. Chem. 288:13305-13316(2013).
RN   [13]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX   PubMed=22936354; DOI=10.1002/jbmr.1751;
RA   Ehlen H.W., Chinenkova M., Moser M., Munter H.M., Krause Y., Gross S.,
RA   Brachvogel B., Wuelling M., Kornak U., Vortkamp A.;
RT   "Inactivation of Anoctamin-6/Tmem16f, a regulator of phosphatidylserine
RT   scrambling in osteoblasts, leads to decreased mineral deposition in
RT   skeletal tissues.";
RL   J. Bone Miner. Res. 28:246-259(2013).
RN   [14]
RP   FUNCTION, CATALYTIC ACTIVITY, AND MUTAGENESIS OF ASP-409; PHE-518; TYR-563;
RP   ILE-612 AND ASP-703.
RX   PubMed=31015464; DOI=10.1038/s41467-019-09778-7;
RA   Le T., Jia Z., Le S.C., Zhang Y., Chen J., Yang H.;
RT   "An inner activation gate controls TMEM16F phospholipid scrambling.";
RL   Nat. Commun. 10:1846-1846(2019).
RN   [15] {ECO:0007744|PDB:6QP6, ECO:0007744|PDB:6QPB, ECO:0007744|PDB:6QPC, ECO:0007744|PDB:6QPI}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.20 ANGSTROMS), FUNCTION, SUBUNIT,
RP   SUBCELLULAR LOCATION, TOPOLOGY, DISULFIDE BONDS, AND MUTAGENESIS OF
RP   ARG-478; GLY-615; GLU-624 AND GLU-667.
RX   PubMed=30785399; DOI=10.7554/elife.44365;
RA   Alvadia C., Lim N.K., Clerico Mosina V., Oostergetel G.T., Dutzler R.,
RA   Paulino C.;
RT   "Cryo-EM structures and functional characterization of the murine lipid
RT   scramblase TMEM16F.";
RL   Elife 8:0-0(2019).
CC   -!- FUNCTION: Small-conductance calcium-activated nonselective cation
CC       (SCAN) channel which acts as a regulator of phospholipid scrambling in
CC       platelets, osteoblasts and fetal thymocytes. Phospholipid scrambling
CC       results in surface exposure of phosphatidylserine which in platelets is
CC       essential to trigger the clotting system whereas in osteoblasts is
CC       essential for the deposition of hydroxyapatite during bone
CC       mineralization. Has calcium-dependent phospholipid scramblase activity;
CC       scrambles phosphatidylserine, phosphatidylcholine and
CC       galactosylceramide. Can generate outwardly rectifying chloride channel
CC       currents in airway epithelial cells and Jurkat T lymphocytes.
CC       {ECO:0000269|PubMed:21107324, ECO:0000269|PubMed:21908539,
CC       ECO:0000269|PubMed:22936354, ECO:0000269|PubMed:23021219,
CC       ECO:0000269|PubMed:23532839, ECO:0000269|PubMed:30785399,
CC       ECO:0000269|PubMed:31015464}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-
CC         sn-glycero-3-phospho-L-serine(out); Xref=Rhea:RHEA:38663,
CC         ChEBI:CHEBI:57262; Evidence={ECO:0000269|PubMed:23532839,
CC         ECO:0000269|PubMed:31015464};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38664;
CC         Evidence={ECO:0000305|PubMed:23532839};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine(out) = a
CC         beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine(in);
CC         Xref=Rhea:RHEA:38899, ChEBI:CHEBI:18390;
CC         Evidence={ECO:0000269|PubMed:23532839};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38900;
CC         Evidence={ECO:0000305|PubMed:23532839};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-
CC         sn-glycero-3-phosphocholine(out); Xref=Rhea:RHEA:38571,
CC         ChEBI:CHEBI:57643; Evidence={ECO:0000269|PubMed:23532839};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:38573;
CC         Evidence={ECO:0000305|PubMed:23532839};
CC   -!- ACTIVITY REGULATION: Exhibits synergistic gating by Ca(2+) and voltage.
CC       Inhibited by some non-specific cation channel blockers such as:
CC       ruthenium red, 2-aminoethyl diphenylborinate (2APB), gadolinium and
CC       cadmium ions. {ECO:0000269|PubMed:23021219}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:30785399}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:30785399};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:30785399}. Note=Shows
CC       an intracellular localization. {ECO:0000250|UniProtKB:Q4KMQ2}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q6P9J9-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q6P9J9-2; Sequence=VSP_015654, VSP_015655;
CC   -!- TISSUE SPECIFICITY: Predominant expression seen in epithelial tissues.
CC       Also found in skeletal system where it is primarily expressed in
CC       osteoblasts. {ECO:0000269|PubMed:20056604,
CC       ECO:0000269|PubMed:22936354}.
CC   -!- DEVELOPMENTAL STAGE: At 14.5 dpc, expressed in lung epithelium and
CC       mesenchyme. At 16.5 dpc, expressed in esophageal epithelium and
CC       mesenchyme. In the caudal digestive tract, detected in small intestine
CC       epithelium at 14.5 dpc. Also detected at 14.5 dpc in epithelium and
CC       mesenchyme of trachea, ovary, kidney and stomach. In the developing
CC       skeleton, expressed in developing rib perichondria at 14.5 dpc. Also
CC       expressed in the neural tube and dorsal root ganglia at 14.5 dpc. In
CC       developing skin, expression is restricted to basal layers of the
CC       epidermis at 16.5 dpc. {ECO:0000269|PubMed:18729231}.
CC   -!- DISRUPTION PHENOTYPE: Mice are viable and fertile, and display no major
CC       morphological defects. They exhibit deficiencies in Ca(2+)-dependent
CC       phospholipid scramblase activity in platelets and defects in blood
CC       coagulation (PubMed:23021219). They also show reduced skeleton size and
CC       skeletal deformities. {ECO:0000269|PubMed:22936354,
CC       ECO:0000269|PubMed:23021219}.
CC   -!- MISCELLANEOUS: The term 'anoctamin' was coined because these channels
CC       are anion selective and are predicted to have eight (OCT) transmembrane
CC       segments. There is some dissatisfaction in the field with the Ano
CC       nomenclature because it is not certain that all the members of this
CC       family are anion channels or have the 8-transmembrane topology.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the anoctamin family. {ECO:0000305}.
CC   -!- CAUTION: Contains ten transmembrane regions, not eight as predicted.
CC       {ECO:0000269|PubMed:30785399}.
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DR   EMBL; AK089300; BAC40833.1; -; mRNA.
DR   EMBL; BC060732; AAH60732.1; -; mRNA.
DR   CCDS; CCDS37184.1; -. [Q6P9J9-1]
DR   RefSeq; NP_001240742.1; NM_001253813.1.
DR   RefSeq; NP_780553.2; NM_175344.4. [Q6P9J9-1]
DR   PDB; 6P46; EM; 3.50 A; A/B=1-911.
DR   PDB; 6P47; EM; 3.90 A; A/B=1-911.
DR   PDB; 6P48; EM; 3.20 A; A/B=1-911.
DR   PDB; 6P49; EM; 3.30 A; A/B=1-911.
DR   PDB; 6QP6; EM; 3.20 A; A/B=1-911.
DR   PDB; 6QPB; EM; 3.60 A; A/B=1-911.
DR   PDB; 6QPC; EM; 3.50 A; A/B=1-911.
DR   PDB; 6QPI; EM; 3.30 A; A/B=1-911.
DR   PDBsum; 6P46; -.
DR   PDBsum; 6P47; -.
DR   PDBsum; 6P48; -.
DR   PDBsum; 6P49; -.
DR   PDBsum; 6QP6; -.
DR   PDBsum; 6QPB; -.
DR   PDBsum; 6QPC; -.
DR   PDBsum; 6QPI; -.
DR   AlphaFoldDB; Q6P9J9; -.
DR   SMR; Q6P9J9; -.
DR   STRING; 10090.ENSMUSP00000071770; -.
DR   SwissLipids; SLP:000000373; -.
DR   GlyGen; Q6P9J9; 6 sites.
DR   iPTMnet; Q6P9J9; -.
DR   PhosphoSitePlus; Q6P9J9; -.
DR   SwissPalm; Q6P9J9; -.
DR   EPD; Q6P9J9; -.
DR   jPOST; Q6P9J9; -.
DR   MaxQB; Q6P9J9; -.
DR   PaxDb; Q6P9J9; -.
DR   PeptideAtlas; Q6P9J9; -.
DR   PRIDE; Q6P9J9; -.
DR   ProteomicsDB; 281994; -. [Q6P9J9-1]
DR   ProteomicsDB; 281995; -. [Q6P9J9-2]
DR   ABCD; Q6P9J9; 1 sequenced antibody.
DR   Antibodypedia; 42608; 135 antibodies from 22 providers.
DR   Ensembl; ENSMUST00000071874; ENSMUSP00000071770; ENSMUSG00000064210. [Q6P9J9-1]
DR   GeneID; 105722; -.
DR   KEGG; mmu:105722; -.
DR   UCSC; uc007xju.2; mouse. [Q6P9J9-1]
DR   CTD; 196527; -.
DR   MGI; MGI:2145890; Ano6.
DR   VEuPathDB; HostDB:ENSMUSG00000064210; -.
DR   eggNOG; KOG2514; Eukaryota.
DR   GeneTree; ENSGT00940000158969; -.
DR   HOGENOM; CLU_006685_1_3_1; -.
DR   InParanoid; Q6P9J9; -.
DR   OMA; EKVTPRW; -.
DR   OrthoDB; 1263362at2759; -.
DR   PhylomeDB; Q6P9J9; -.
DR   TreeFam; TF314265; -.
DR   Reactome; R-MMU-2672351; Stimuli-sensing channels.
DR   Reactome; R-MMU-6798695; Neutrophil degranulation.
DR   BioGRID-ORCS; 105722; 2 hits in 73 CRISPR screens.
DR   ChiTaRS; Ano6; mouse.
DR   PRO; PR:Q6P9J9; -.
DR   Proteomes; UP000000589; Chromosome 15.
DR   RNAct; Q6P9J9; protein.
DR   Bgee; ENSMUSG00000064210; Expressed in otolith organ and 253 other tissues.
DR   ExpressionAtlas; Q6P9J9; baseline and differential.
DR   Genevisible; Q6P9J9; MM.
DR   GO; GO:0034707; C:chloride channel complex; ISO:MGI.
DR   GO; GO:0005829; C:cytosol; ISO:MGI.
DR   GO; GO:0005887; C:integral component of plasma membrane; IDA:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR   GO; GO:0005227; F:calcium activated cation channel activity; IDA:UniProtKB.
DR   GO; GO:0005254; F:chloride channel activity; IBA:GO_Central.
DR   GO; GO:0042802; F:identical protein binding; IPI:MGI.
DR   GO; GO:0005229; F:intracellular calcium activated chloride channel activity; IDA:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017128; F:phospholipid scramblase activity; IDA:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR   GO; GO:0005247; F:voltage-gated chloride channel activity; ISO:MGI.
DR   GO; GO:0005244; F:voltage-gated ion channel activity; IDA:UniProtKB.
DR   GO; GO:0002543; P:activation of blood coagulation via clotting cascade; ISO:MGI.
DR   GO; GO:0032060; P:bleb assembly; IMP:UniProtKB.
DR   GO; GO:0007596; P:blood coagulation; IMP:UniProtKB.
DR   GO; GO:0035630; P:bone mineralization involved in bone maturation; IMP:MGI.
DR   GO; GO:0061591; P:calcium activated galactosylceramide scrambling; IDA:MGI.
DR   GO; GO:0061590; P:calcium activated phosphatidylcholine scrambling; IDA:MGI.
DR   GO; GO:0061589; P:calcium activated phosphatidylserine scrambling; IDA:MGI.
DR   GO; GO:0061588; P:calcium activated phospholipid scrambling; IMP:MGI.
DR   GO; GO:0070588; P:calcium ion transmembrane transport; IGI:UniProtKB.
DR   GO; GO:0006812; P:cation transport; ISO:MGI.
DR   GO; GO:1902476; P:chloride transmembrane transport; IDA:UniProtKB.
DR   GO; GO:0006821; P:chloride transport; IDA:MGI.
DR   GO; GO:0002407; P:dendritic cell chemotaxis; IMP:MGI.
DR   GO; GO:0051649; P:establishment of localization in cell; IDA:MGI.
DR   GO; GO:0045794; P:negative regulation of cell volume; IMP:UniProtKB.
DR   GO; GO:0097045; P:phosphatidylserine exposure on blood platelet; ISO:MGI.
DR   GO; GO:0045332; P:phospholipid translocation; IDA:UniProtKB.
DR   GO; GO:0017121; P:plasma membrane phospholipid scrambling; IMP:UniProtKB.
DR   GO; GO:0046931; P:pore complex assembly; ISO:MGI.
DR   GO; GO:0043065; P:positive regulation of apoptotic process; IMP:UniProtKB.
DR   GO; GO:0030501; P:positive regulation of bone mineralization; IMP:UniProtKB.
DR   GO; GO:2000353; P:positive regulation of endothelial cell apoptotic process; ISO:MGI.
DR   GO; GO:0034767; P:positive regulation of ion transmembrane transport; IMP:UniProtKB.
DR   GO; GO:0090026; P:positive regulation of monocyte chemotaxis; IMP:UniProtKB.
DR   GO; GO:0060100; P:positive regulation of phagocytosis, engulfment; IMP:UniProtKB.
DR   GO; GO:1903766; P:positive regulation of potassium ion export across plasma membrane; ISS:BHF-UCL.
DR   GO; GO:0035590; P:purinergic nucleotide receptor signaling pathway; IMP:UniProtKB.
DR   GO; GO:0035725; P:sodium ion transmembrane transport; ISO:MGI.
DR   GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR   InterPro; IPR032394; Anoct_dimer.
DR   InterPro; IPR007632; Anoctamin.
DR   InterPro; IPR031295; Anoctamin-6.
DR   PANTHER; PTHR12308; PTHR12308; 1.
DR   PANTHER; PTHR12308:SF21; PTHR12308:SF21; 1.
DR   Pfam; PF16178; Anoct_dimer; 1.
DR   Pfam; PF04547; Anoctamin; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; Calcium; Cell membrane; Chloride;
KW   Chloride channel; Disulfide bond; Glycoprotein; Ion channel; Ion transport;
KW   Lipid transport; Membrane; Metal-binding; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport; Voltage-gated channel.
FT   CHAIN           1..911
FT                   /note="Anoctamin-6"
FT                   /id="PRO_0000191758"
FT   TOPO_DOM        1..301
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TRANSMEM        302..322
FT                   /note="Helical"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TOPO_DOM        323..376
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TRANSMEM        377..397
FT                   /note="Helical"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TOPO_DOM        398..456
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TRANSMEM        457..477
FT                   /note="Helical"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TOPO_DOM        478..510
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TRANSMEM        511..531
FT                   /note="Helical"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TOPO_DOM        532..552
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TRANSMEM        553..573
FT                   /note="Helical"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TOPO_DOM        574..602
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TRANSMEM        603..622
FT                   /note="Helical"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TOPO_DOM        623..664
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TRANSMEM        665..685
FT                   /note="Helical"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TRANSMEM        686..706
FT                   /note="Helical"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TOPO_DOM        707..723
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TRANSMEM        724..744
FT                   /note="Helical"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TOPO_DOM        745..837
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TRANSMEM        838..858
FT                   /note="Helical"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   TOPO_DOM        859..911
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   BINDING         624
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000305|PubMed:30785399"
FT   BINDING         667
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000305|PubMed:30785399"
FT   BINDING         670
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000305|PubMed:30785399"
FT   CARBOHYD        330
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        362
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        494
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:19656770"
FT   CARBOHYD        778
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        785
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:19656770"
FT   CARBOHYD        803
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:19656770"
FT   DISULFID        331..372
FT                   /evidence="ECO:0000269|PubMed:30785399,
FT                   ECO:0007744|PDB:6QP6, ECO:0007744|PDB:6QPC"
FT   DISULFID        338..365
FT                   /evidence="ECO:0000269|PubMed:30785399,
FT                   ECO:0007744|PDB:6QP6, ECO:0007744|PDB:6QPB,
FT                   ECO:0007744|PDB:6QPC"
FT   DISULFID        349..807
FT                   /evidence="ECO:0000269|PubMed:30785399,
FT                   ECO:0007744|PDB:6QP6, ECO:0007744|PDB:6QPB,
FT                   ECO:0007744|PDB:6QPC"
FT   DISULFID        352..356
FT                   /evidence="ECO:0000269|PubMed:30785399,
FT                   ECO:0007744|PDB:6QPC"
FT   DISULFID        596..601
FT                   /evidence="ECO:0000269|PubMed:30785399,
FT                   ECO:0007744|PDB:6QP6, ECO:0007744|PDB:6QPB,
FT                   ECO:0007744|PDB:6QPC"
FT   VAR_SEQ         1..427
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_015654"
FT   VAR_SEQ         428..436
FT                   /note="NHVVINEIT -> MALMAESLC (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_015655"
FT   MUTAGEN         370
FT                   /note="K->A: No effect on lipid scramblase activity."
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   MUTAGEN         409
FT                   /note="D->G: Increased speed of phospholipid scrambling."
FT                   /evidence="ECO:0000269|PubMed:31015464"
FT   MUTAGEN         409
FT                   /note="D->G: Reduced channel activity and sensitivity to
FT                   Ca(2+)."
FT                   /evidence="ECO:0000269|PubMed:23021219"
FT   MUTAGEN         478
FT                   /note="R->A: Decreased lipid scramblase and ion channel
FT                   activity. Requires lower calcium levels for activation of
FT                   ion channel activity."
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   MUTAGEN         518
FT                   /note="F->A: Increased speed of phospholipid scrambling.
FT                   Constitutive scramblase activity at basal cytosolic calcium
FT                   levels; when associated with A-563 and A-612."
FT                   /evidence="ECO:0000269|PubMed:31015464"
FT   MUTAGEN         518
FT                   /note="F->E,K,Q: Constitutive lipid scramblase activity at
FT                   basal cytosolic calcium levels."
FT                   /evidence="ECO:0000269|PubMed:31015464"
FT   MUTAGEN         518
FT                   /note="F->L: Decreased lipid scramblase activity."
FT                   /evidence="ECO:0000269|PubMed:31015464"
FT   MUTAGEN         518
FT                   /note="F->W: Slightly increased lipid scramblase activity."
FT                   /evidence="ECO:0000269|PubMed:31015464"
FT   MUTAGEN         559
FT                   /note="Q->K: Moderately decreased sensitivity to activation
FT                   by calcium."
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   MUTAGEN         559
FT                   /note="Q->K: Slower channel activation. Increased
FT                   permeability to chloride ions."
FT                   /evidence="ECO:0000269|PubMed:23021219"
FT   MUTAGEN         563
FT                   /note="Y->A: Increased speed of phospholipid scrambling.
FT                   Requires lower calcium levels for activation of scramblase
FT                   and ion channel activity. Constitutive scramblase activity
FT                   at basal cytosolic calcium levels; when associated with A-
FT                   518 and A-612."
FT                   /evidence="ECO:0000269|PubMed:31015464"
FT   MUTAGEN         563
FT                   /note="Y->K,Q: Constitutive lipid scramblase activity at
FT                   basal cytosolic calcium levels."
FT                   /evidence="ECO:0000269|PubMed:31015464"
FT   MUTAGEN         563
FT                   /note="Y->S: No effect on lipid scramblase activity."
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   MUTAGEN         563
FT                   /note="Y->W: Loss of lipid scramblase activity."
FT                   /evidence="ECO:0000269|PubMed:31015464"
FT   MUTAGEN         612
FT                   /note="I->A: Increased speed of phospholipid scrambling.
FT                   Constitutive scramblase activity at basal cytosolic calcium
FT                   levels; when associated with A-518 and A-563."
FT                   /evidence="ECO:0000269|PubMed:31015464"
FT   MUTAGEN         612
FT                   /note="I->E,K: Constitutive lipid scramblase activity at
FT                   basal cytosolic calcium levels."
FT                   /evidence="ECO:0000269|PubMed:31015464"
FT   MUTAGEN         612
FT                   /note="I->W: Decreased lipid scramblase activity."
FT                   /evidence="ECO:0000269|PubMed:31015464"
FT   MUTAGEN         615
FT                   /note="G->A: Requires lower calcium levels for activation
FT                   of scramblase and ion channel activity."
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   MUTAGEN         623
FT                   /note="Q->F: No effect on lipid scramblase activity."
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   MUTAGEN         624
FT                   /note="E->Q: Expected to disrupt calcium binding. Loss of
FT                   scramblase and ion channel activity."
FT                   /evidence="ECO:0000269|PubMed:30785399"
FT   MUTAGEN         667
FT                   /note="E->Q: Requires much higher calcium levels for the
FT                   activation of scramblase and ion channel activity."
FT                   /evidence="ECO:0000269|PubMed:23021219,
FT                   ECO:0000269|PubMed:30785399"
FT   MUTAGEN         703
FT                   /note="D->R: Expected to disrupt calcium binding. Loss of
FT                   scramblase activity."
FT                   /evidence="ECO:0000269|PubMed:31015464"
FT   STRAND          45..47
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   STRAND          67..70
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   STRAND          73..76
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           91..103
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          111..116
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   STRAND          121..129
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   TURN            137..141
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          146..148
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   STRAND          188..192
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   HELIX           206..219
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           234..238
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          243..247
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          253..255
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   STRAND          260..262
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   HELIX           268..271
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   TURN            272..274
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           276..278
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   STRAND          279..281
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   HELIX           286..289
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   TURN            290..296
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           297..309
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           312..325
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          327..329
FT                   /evidence="ECO:0007829|PDB:6P46"
FT   STRAND          330..335
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   TURN            336..338
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   TURN            340..342
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          343..345
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          351..355
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          361..370
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           372..375
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   TURN            378..381
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           382..407
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           421..426
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   HELIX           452..455
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           457..479
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           481..485
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           505..536
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          537..539
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           544..550
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           552..572
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          574..577
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   STRAND          581..583
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   HELIX           586..588
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          589..591
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           602..613
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           616..619
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   TURN            620..628
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          629..632
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           639..645
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           650..653
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          655..657
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   HELIX           665..678
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   TURN            680..684
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   HELIX           688..708
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          720..722
FT                   /evidence="ECO:0007829|PDB:6QPC"
FT   HELIX           726..746
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          748..750
FT                   /evidence="ECO:0007829|PDB:6P46"
FT   HELIX           751..759
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          761..763
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   TURN            778..780
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          781..786
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   HELIX           798..801
FT                   /evidence="ECO:0007829|PDB:6QP6"
FT   STRAND          808..811
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   STRAND          816..818
FT                   /evidence="ECO:0007829|PDB:6P49"
FT   TURN            819..822
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           826..856
FT                   /evidence="ECO:0007829|PDB:6P48"
FT   HELIX           862..874
FT                   /evidence="ECO:0007829|PDB:6QP6"
SQ   SEQUENCE   911 AA;  106255 MW;  33D7A6EF02635B39 CRC64;
     MQMMTRKVLL NMELEEDDDE DGDIVLENFD QTIVCPTFGS LENQQDFRTP EFEEFNGKPD
     SLFFTDGQRR IDFILVYEDE SKKENNKKGT NEKQKRKRQA YESNLICHGL QLEATRSVSD
     DKLVFVKVHA PWEVLCTYAE IMHIKLPLKP NDLKTRSPFG NLNWFTKVLR VNESVIKPEQ
     EFFTAPFEKS RMNDFYILDR DSFFNPATRS RIVYFILSRV KYQVMNNVNK FGINRLVSSG
     IYKAAFPLHD CRFNYESEDI SCPSERYLLY REWAHPRSIY KKQPLDLIRK YYGEKIGIYF
     AWLGYYTQML LLAAVVGVAC FLYGYLDQDN CTWSKEVCDP DIGGQILMCP QCDRLCPFWR
     LNITCESSKK LCIFDSFGTL IFAVFMGVWV TLFLEFWKRR QAELEYEWDT VELQQEEQAR
     PEYEAQCNHV VINEITQEEE RIPFTTCGKC IRVTLCASAV FFWILLIIAS VIGIIVYRLS
     VFIVFSTTLP KNPNGTDPIQ KYLTPQMATS ITASIISFII IMILNTIYEK VAIMITNFEL
     PRTQTDYENS LTMKMFLFQF VNYYSSCFYI AFFKGKFVGY PGDPVYLLGK YRSEECDPGG
     CLLELTTQLT IIMGGKAIWN NIQEVLLPWV MNLIGRYKRV SGSEKITPRW EQDYHLQPMG
     KLGLFYEYLE MIIQFGFVTL FVASFPLAPL LALVNNILEI RVDAWKLTTQ FRRMVPEKAQ
     DIGAWQPIMQ GIAILAVVTN AMIIAFTSDM IPRLVYYWSF SIPPYGDHTY YTMDGYINNT
     LSVFNITDFK NTDKENPYIG LGNYTLCRYR DFRNPPGHPQ EYKHNIYYWH VIAAKLAFII
     VMEHIIYSVK FFISYAIPDV SKITKSKIKR EKYLTQKLLH ESHLKDLTKN MGIIAERIGG
     TVDNSVRPKL E
 
 
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