ANO7_MOUSE
ID ANO7_MOUSE Reviewed; 859 AA.
AC Q14AT5; Q6IFT5;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Anoctamin-7;
DE AltName: Full=New gene expressed in prostate homolog;
DE AltName: Full=Transmembrane protein 16G;
GN Name=Ano7; Synonyms=Ngep, Tmem16g;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION.
RX PubMed=14981236; DOI=10.1073/pnas.0308746101;
RA Bera T.K., Das S., Maeda H., Beers R., Wolfgang C.D., Kumar V., Hahn Y.,
RA Lee B., Pastan I.;
RT "NGEP, a gene encoding a membrane protein detected only in prostate cancer
RT and normal prostate.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:3059-3064(2004).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=20056604; DOI=10.1074/jbc.m109.065367;
RA Schreiber R., Uliyakina I., Kongsuphol P., Warth R., Mirza M.,
RA Martins J.R., Kunzelmann K.;
RT "Expression and function of epithelial anoctamins.";
RL J. Biol. Chem. 285:7838-7845(2010).
RN [5]
RP REVIEW.
RX PubMed=22302790; DOI=10.1113/expphysiol.2011.058214;
RA Winpenny J.P., Gray M.A.;
RT "The anoctamin (TMEM16) gene family: calcium-activated chloride channels
RT come of age.";
RL Exp. Physiol. 97:175-176(2012).
RN [6]
RP FUNCTION, CATALYTIC ACTIVITY, AND TISSUE SPECIFICITY.
RX PubMed=23532839; DOI=10.1074/jbc.m113.457937;
RA Suzuki J., Fujii T., Imao T., Ishihara K., Kuba H., Nagata S.;
RT "Calcium-dependent phospholipid scramblase activity of TMEM16 protein
RT family members.";
RL J. Biol. Chem. 288:13305-13316(2013).
CC -!- FUNCTION: Has calcium-dependent phospholipid scramblase activity;
CC scrambles phosphatidylserine, phosphatidylcholine and
CC galactosylceramide (PubMed:23532839). Does not exhibit calcium-
CC activated chloride channel (CaCC) activity (PubMed:23532839). May play
CC a role in cell-cell interactions (By similarity).
CC {ECO:0000250|UniProtKB:Q6IWH7, ECO:0000269|PubMed:23532839}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-
CC sn-glycero-3-phospho-L-serine(out); Xref=Rhea:RHEA:38663,
CC ChEBI:CHEBI:57262; Evidence={ECO:0000269|PubMed:23532839};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38664;
CC Evidence={ECO:0000305|PubMed:23532839};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine(out) = a
CC beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine(in);
CC Xref=Rhea:RHEA:38899, ChEBI:CHEBI:18390;
CC Evidence={ECO:0000269|PubMed:23532839};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38900;
CC Evidence={ECO:0000305|PubMed:23532839};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-
CC sn-glycero-3-phosphocholine(out); Xref=Rhea:RHEA:38571,
CC ChEBI:CHEBI:57643; Evidence={ECO:0000269|PubMed:23532839};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:38573;
CC Evidence={ECO:0000305|PubMed:23532839};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q6IWH7};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:Q6IWH7}. Endoplasmic
CC reticulum {ECO:0000250|UniProtKB:Q6IWH7}. Note=Concentrates at sites of
CC cell-cell contact. Shows an intracellular localization.
CC {ECO:0000250|UniProtKB:Q6IWH7}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q14AT5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q14AT5-2; Sequence=VSP_026009, VSP_026010;
CC -!- TISSUE SPECIFICITY: Highly expressed in the stomach. Expressed at low
CC levels in small intestine and large intestine.
CC {ECO:0000269|PubMed:20056604, ECO:0000269|PubMed:23532839}.
CC -!- MISCELLANEOUS: The term 'anoctamin' was coined because these channels
CC are anion selective and have eight (OCT) transmembrane segments. There
CC is some dissatisfaction in the field with the Ano nomenclature because
CC it is not certain that all the members of this family are anion
CC channels or have the 8-transmembrane topology.
CC -!- SIMILARITY: Belongs to the anoctamin family. {ECO:0000305}.
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DR EMBL; AC108412; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC124669; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC116706; AAI16707.1; -; mRNA.
DR EMBL; BK004075; DAA04566.1; -; mRNA.
DR CCDS; CCDS15188.1; -. [Q14AT5-1]
DR CCDS; CCDS78656.1; -. [Q14AT5-2]
DR RefSeq; NP_001258813.1; NM_001271884.1. [Q14AT5-2]
DR RefSeq; NP_996914.1; NM_207031.2. [Q14AT5-1]
DR RefSeq; XP_006529796.1; XM_006529733.2. [Q14AT5-1]
DR AlphaFoldDB; Q14AT5; -.
DR SMR; Q14AT5; -.
DR BioGRID; 240364; 3.
DR STRING; 10090.ENSMUSP00000140438; -.
DR SwissLipids; SLP:000000375; -.
DR GlyGen; Q14AT5; 2 sites.
DR iPTMnet; Q14AT5; -.
DR PhosphoSitePlus; Q14AT5; -.
DR PaxDb; Q14AT5; -.
DR PRIDE; Q14AT5; -.
DR ProteomicsDB; 296311; -. [Q14AT5-1]
DR ProteomicsDB; 296312; -. [Q14AT5-2]
DR Antibodypedia; 47723; 121 antibodies from 26 providers.
DR DNASU; 404545; -.
DR Ensembl; ENSMUST00000058682; ENSMUSP00000050495; ENSMUSG00000034107. [Q14AT5-2]
DR Ensembl; ENSMUST00000186641; ENSMUSP00000140438; ENSMUSG00000034107. [Q14AT5-1]
DR GeneID; 404545; -.
DR KEGG; mmu:404545; -.
DR UCSC; uc007cdw.2; mouse. [Q14AT5-1]
DR UCSC; uc033fku.1; mouse. [Q14AT5-2]
DR CTD; 50636; -.
DR MGI; MGI:3052714; Ano7.
DR VEuPathDB; HostDB:ENSMUSG00000034107; -.
DR eggNOG; KOG2514; Eukaryota.
DR GeneTree; ENSGT00940000158551; -.
DR HOGENOM; CLU_006685_0_1_1; -.
DR InParanoid; Q14AT5; -.
DR OMA; RWAMTSE; -.
DR OrthoDB; 1263362at2759; -.
DR PhylomeDB; Q14AT5; -.
DR TreeFam; TF314265; -.
DR Reactome; R-MMU-2672351; Stimuli-sensing channels.
DR BioGRID-ORCS; 404545; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q14AT5; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q14AT5; protein.
DR Bgee; ENSMUSG00000034107; Expressed in duodenum and 32 other tissues.
DR ExpressionAtlas; Q14AT5; baseline and differential.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0005254; F:chloride channel activity; IBA:GO_Central.
DR GO; GO:0017128; F:phospholipid scramblase activity; IDA:MGI.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0061591; P:calcium activated galactosylceramide scrambling; IDA:MGI.
DR GO; GO:0061590; P:calcium activated phosphatidylcholine scrambling; IDA:MGI.
DR GO; GO:0061589; P:calcium activated phosphatidylserine scrambling; IDA:MGI.
DR GO; GO:0061588; P:calcium activated phospholipid scrambling; IBA:GO_Central.
DR GO; GO:1902476; P:chloride transmembrane transport; IBA:GO_Central.
DR GO; GO:0006821; P:chloride transport; ISO:MGI.
DR GO; GO:0051649; P:establishment of localization in cell; IDA:MGI.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR InterPro; IPR032394; Anoct_dimer.
DR InterPro; IPR007632; Anoctamin.
DR InterPro; IPR031296; Anoctamin-7.
DR PANTHER; PTHR12308; PTHR12308; 1.
DR PANTHER; PTHR12308:SF22; PTHR12308:SF22; 1.
DR Pfam; PF16178; Anoct_dimer; 1.
DR Pfam; PF04547; Anoctamin; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Endoplasmic reticulum; Glycoprotein;
KW Lipid transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..859
FT /note="Anoctamin-7"
FT /id="PRO_0000289327"
FT TOPO_DOM 1..297
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 319..362
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 363..383
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 384..441
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..492
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 493..513
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 514..530
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 531..551
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 552..651
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 652..672
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 673..700
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 701..721
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 722..780
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 781..801
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 802..859
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 25..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..47
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 746
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 761
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 836..843
FT /note="ALLGATGV -> VTVGVTGG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026009"
FT VAR_SEQ 844..859
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026010"
SQ SEQUENCE 859 AA; 97128 MW; 82E1A473C59C8DA3 CRC64;
MLRGQAREED SVVLIDMASP EAGNGCSYGS TAQASEAGKQ QVAPSRVGSS AKPPIDFVLV
WEEDLRNQEN PTKDKTDTHE VWRETFLENL CLAGLKIDQH DVQDEAAAVH YILLRAPWAV
LCYYAEDLRL KLPLQELPNQ ASNWSATLLE WLGIPNILLE HVPDTPPEYY SCQFKASKLQ
WFLGSDNQDT FFTSTKRHQI LFEILAKTPY GHEKKGLFGI DQLLAEGVFS AAFPLHDGPF
SAVPESSQVL GLIQRQVLFQ HWARWGKWNK YQPLDHVRRY FGEKVALYFA WLGFYTGWLL
PAAVVGTVVF LVGCFLVFSD IPTQELCHSS DSFDMCPLCS DCSFWLLSSA CTLAQAGRLF
DHGGTVFFSL FMALWAVLLL EYWKRKNATL AYRWDCSDYE DIEERPRPQF AATAPMTALN
PITGEDEPYF PEKNRVRRML AGSVVLLMMV AVVIMCLVSV ILYRAVMAII VSRSDNAFLS
AWASRIASLT GSVVNLVFIL ILSKVYVLLA QVLTRWEMHR TQTEFEDAFT LKVFIFQFVN
FYASPVYIAF FKGRFVGYPG NYHTLFGIRN EECPAGGCLS ELAQELLVIM VGKQIINNVQ
EVLVPKLKGC WQKFSRGKKA GTGTHPAPWE ADYELLPCEG LFHEYLEMVL QFGFVTIFVA
ACPLAPLFAL LNNWVEIRLD ARKFVCEYRR PVAERAQDIG IWFHILTGLT HLAVISNAFL
LAFSSDFLPR VYYSWTHAPD LHGFLNFTLA RAPPTFTSAH NRTCRYRAFR DDDGHYSPTY
WTLLAIRLAF VIVFEHVVFS IGRVLDLLVP DIPESVEIKV KREYYLAKQA LAENEALLGA
TGVKDDQPPS SEPSLGLPA
