ID   ANP11_ZOAAM             Reviewed;          62 AA.
AC   P19613;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Ice-structuring protein SP2(HPLC 11);
DE            Short=ISP SP2(HPLC 11);
DE   AltName: Full=Antifreeze protein SP2(HPLC 11);
OS   Zoarces americanus (Ocean pout) (Macrozoarces americanus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Perciformes; Cottioidei; Zoarcales; Zoarcidae; Zoarcinae;
OC   Zoarces.
OX   NCBI_TaxID=8199;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=3403560; DOI=10.1016/s0021-9258(18)37891-8;
RA   Hew C.-L., Wang N.-C., Joshi S., Fletcher G.L., Scott G.K., Hayes P.H.,
RA   Buettner B., Davies P.L.;
RT   "Multiple genes provide the basis for antifreeze protein diversity and
RT   dosage in the ocean pout, Macrozoarces americanus.";
RL   J. Biol. Chem. 263:12049-12055(1988).
CC   -!- FUNCTION: Contributes to protect fish blood from freezing at subzero
CC       sea water temperatures. Lowers the blood freezing point. Binds to
CC       nascent ice crystals and prevents further growth (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:3403560}.
CC   -!- TISSUE SPECIFICITY: Detected in blood serum (at protein level).
CC       {ECO:0000269|PubMed:3403560}.
CC   -!- SIMILARITY: Belongs to the type-III AFP family. {ECO:0000305}.
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DR   PIR; I31075; I31075.
DR   AlphaFoldDB; P19613; -.
DR   SMR; P19613; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR006190; AFP_Neu5c_C.
DR   InterPro; IPR036732; AFP_Neu5c_C_sf.
DR   InterPro; IPR006013; Antifreeze_III.
DR   InterPro; IPR013974; SAF.
DR   Pfam; PF08666; SAF; 1.
DR   PRINTS; PR00357; ANTIFREEZIII.
DR   SUPFAM; SSF51269; SSF51269; 1.
DR   PROSITE; PS50844; AFP_LIKE; 1.
PE   1: Evidence at protein level;
KW   Antifreeze protein; Direct protein sequencing; Secreted.
FT   CHAIN           1..62
FT                   /note="Ice-structuring protein SP2(HPLC 11)"
FT                   /id="PRO_0000155156"
FT   DOMAIN          1..60
FT                   /note="AFP-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00021"
FT   SITE            6
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            11
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            15
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            41
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   62 AA;  6585 MW;  3F8B94CDE6977E89 CRC64;
     SVVATQLIPI NTALTPAMME GKVTNPIGIP FAEMSQIVGK QVNRIVAKGQ TLMPNMVKTY
     AA