ID   3SO8_NAJKA              Reviewed;          65 AA.
AC   P82464;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2000, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Muscarinic toxin-like protein 3;
DE            Short=MTLP-3;
OS   Naja kaouthia (Monocled cobra) (Naja siamensis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8649;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=11082188; DOI=10.1046/j.1432-1033.2000.01775.x;
RA   Kukhtina V.V., Weise C., Muranova T.A., Starkov V.G., Franke P., Hucho F.,
RA   Wnendt S., Gillen C., Tsetlin V.I., Utkin Y.N.;
RT   "Muscarinic toxin-like proteins from cobra venom.";
RL   Eur. J. Biochem. 267:6784-6789(2000).
CC   -!- FUNCTION: Antagonist of muscle and neuronal nicotinic acetylcholine
CC       receptors (nAChR) with highest affinity for neuronal alpha-7/CHRNA7
CC       nAChRs. {ECO:0000250|UniProtKB:A8N286}.
CC   -!- SUBUNIT: Homodimer; non-covalently linked.
CC       {ECO:0000250|UniProtKB:A8N286}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11082188}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=7615; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:11082188};
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Orphan group VIII (haditoxin) sub-subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P82464; -.
DR   SMR; P82464; -.
DR   PRIDE; P82464; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond;
KW   G-protein coupled acetylcholine receptor impairing toxin;
KW   G-protein coupled receptor impairing toxin; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Toxin.
FT   CHAIN           1..65
FT                   /note="Muscarinic toxin-like protein 3"
FT                   /evidence="ECO:0000269|PubMed:11082188"
FT                   /id="PRO_0000093652"
FT   DISULFID        3..24
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        17..41
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        45..57
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        58..63
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
SQ   SEQUENCE   65 AA;  7624 MW;  F16934FD5BA28E4F CRC64;
     TICYNHLTRT SETTEICPDS WYFCYKISLA DGNDVRIKRG CTFTCPELRP TGIYVYCCRR
     DKCNQ