ID   ANP1_LYCPO              Reviewed;          66 AA.
AC   P24028;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Ice-structuring protein LP;
DE            Short=ISP LP;
DE   AltName: Full=Antifreeze protein LP;
OS   Lycodes polaris (Canadian eelpout).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Perciformes; Cottioidei; Zoarcales; Zoarcidae; Lycodinae;
OC   Lycodes.
OX   NCBI_TaxID=8197;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=3477289; DOI=10.1016/0167-4838(87)90021-5;
RA   Schrag J.D., Cheng C.-H.C., Panico M., Morris H.R., Devries A.L.;
RT   "Primary and secondary structure of antifreeze peptides from arctic and
RT   antarctic zoarcid fishes.";
RL   Biochim. Biophys. Acta 915:357-370(1987).
CC   -!- FUNCTION: Contributes to protect fish blood from freezing at subzero
CC       sea water temperatures. Lowers the blood freezing point. Binds to
CC       nascent ice crystals and prevents further growth (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the type-III AFP family. {ECO:0000305}.
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DR   PIR; S07150; FDFILC.
DR   AlphaFoldDB; P24028; -.
DR   SMR; P24028; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR006190; AFP_Neu5c_C.
DR   InterPro; IPR036732; AFP_Neu5c_C_sf.
DR   InterPro; IPR006013; Antifreeze_III.
DR   PRINTS; PR00357; ANTIFREEZIII.
DR   SUPFAM; SSF51269; SSF51269; 1.
DR   PROSITE; PS50844; AFP_LIKE; 1.
PE   1: Evidence at protein level;
KW   Antifreeze protein; Direct protein sequencing; Secreted.
FT   CHAIN           1..66
FT                   /note="Ice-structuring protein LP"
FT                   /id="PRO_0000155154"
FT   DOMAIN          4..63
FT                   /note="AFP-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00021"
FT   SITE            9
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            14
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            18
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            44
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   66 AA;  6982 MW;  6F06E38A329767EC CRC64;
     NKASVVANQL IPINTALTLV MMRAEVVTPA GIPAEDIPRL VGLQVNRAVL IGTTLMPDMV
     KGYAPQ