ID   ANP2_PACBR              Reviewed;          63 AA.
AC   P12101;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Ice-structuring protein AB2;
DE            Short=ISP AB2;
DE   AltName: Full=Antifreeze peptide AB2;
OS   Pachycara brachycephalum (Antarctic eelpout) (Austrolycichthys
OS   brachycephalus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Perciformes; Cottioidei; Zoarcales; Zoarcidae; Lycodinae;
OC   Pachycara.
OX   NCBI_TaxID=36221;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=2752054; DOI=10.1016/0167-4838(89)90135-0;
RA   Cheng C.-H.C., Devries A.L.;
RT   "Structures of antifreeze peptides from the antarctic eel pout,
RT   Austrolycicthys brachycephalus.";
RL   Biochim. Biophys. Acta 997:55-64(1989).
CC   -!- FUNCTION: Contributes to protect fish blood from freezing at subzero
CC       sea water temperatures. Lowers the blood freezing point. Binds to
CC       nascent ice crystals and prevents further growth (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:2752054}.
CC   -!- TISSUE SPECIFICITY: Detected in blood serum (at protein level).
CC       {ECO:0000269|PubMed:2752054}.
CC   -!- SIMILARITY: Belongs to the type-III AFP family. {ECO:0000305}.
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DR   PIR; S04974; S04974.
DR   AlphaFoldDB; P12101; -.
DR   SMR; P12101; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR006190; AFP_Neu5c_C.
DR   InterPro; IPR036732; AFP_Neu5c_C_sf.
DR   InterPro; IPR006013; Antifreeze_III.
DR   InterPro; IPR013974; SAF.
DR   Pfam; PF08666; SAF; 1.
DR   PRINTS; PR00357; ANTIFREEZIII.
DR   SUPFAM; SSF51269; SSF51269; 1.
DR   PROSITE; PS50844; AFP_LIKE; 1.
PE   1: Evidence at protein level;
KW   Antifreeze protein; Direct protein sequencing; Secreted.
FT   CHAIN           1..63
FT                   /note="Ice-structuring protein AB2"
FT                   /id="PRO_0000155150"
FT   DOMAIN          3..62
FT                   /note="AFP-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00021"
FT   SITE            8
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            13
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            17
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            43
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   63 AA;  7001 MW;  A1E7F954598DD061 CRC64;
     TKSVVANQLI PINTALTLVM MKAEEVSPKG IPAEEIPRLV GMQVNRAVYL DETLMPDMVK
     NYE