ID   ANP3_ZOAAM              Reviewed;          91 AA.
AC   P19606;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Ice-structuring protein lambda OP-3;
DE            Short=ISP lambda OP-3;
DE   AltName: Full=Antifreeze protein lambda OP-3;
DE   Flags: Precursor;
OS   Zoarces americanus (Ocean pout) (Macrozoarces americanus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Perciformes; Cottioidei; Zoarcales; Zoarcidae; Zoarcinae;
OC   Zoarces.
OX   NCBI_TaxID=8199;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3403560; DOI=10.1016/s0021-9258(18)37891-8;
RA   Hew C.-L., Wang N.-C., Joshi S., Fletcher G.L., Scott G.K., Hayes P.H.,
RA   Buettner B., Davies P.L.;
RT   "Multiple genes provide the basis for antifreeze protein diversity and
RT   dosage in the ocean pout, Macrozoarces americanus.";
RL   J. Biol. Chem. 263:12049-12055(1988).
CC   -!- FUNCTION: Contributes to protect fish blood from freezing at subzero
CC       sea water temperatures. Lowers the blood freezing point. Binds to
CC       nascent ice crystals and prevents further growth (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the type-III AFP family. {ECO:0000305}.
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DR   EMBL; J03923; AAA49348.1; -; Genomic_DNA.
DR   PIR; B30839; B30839.
DR   AlphaFoldDB; P19606; -.
DR   SMR; P19606; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR006190; AFP_Neu5c_C.
DR   InterPro; IPR036732; AFP_Neu5c_C_sf.
DR   InterPro; IPR006013; Antifreeze_III.
DR   PRINTS; PR00357; ANTIFREEZIII.
DR   SUPFAM; SSF51269; SSF51269; 1.
DR   PROSITE; PS50844; AFP_LIKE; 1.
PE   3: Inferred from homology;
KW   Antifreeze protein; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000250"
FT   CHAIN           23..91
FT                   /note="Ice-structuring protein lambda OP-3"
FT                   /id="PRO_0000001694"
FT   DOMAIN          25..84
FT                   /note="AFP-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00021"
FT   SITE            30
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            35
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            39
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            65
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   91 AA;  9912 MW;  B85C9037F969F7CE CRC64;
     MKSVILTGLL FVLLCVDHMS SANQSVVATQ LIPINTALTL VMMTTRVIYP TGIPAEDIPR
     LVSMQVNQAV PMGTTLMPDM VKFYCLCAPK N