ID   ANP4_PSEAM              Reviewed;          85 AA.
AC   P02734;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Ice-structuring protein 4;
DE            Short=ISP 4;
DE   AltName: Full=Antifreeze peptide 4;
DE   Flags: Precursor;
OS   Pseudopleuronectes americanus (Winter flounder) (Pleuronectes americanus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Carangaria; Pleuronectiformes; Pleuronectoidei; Pleuronectidae;
OC   Pseudopleuronectes.
OX   NCBI_TaxID=8265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=6265915; DOI=10.1073/pnas.78.5.2825;
RA   Lin Y., Gross J.K.;
RT   "Molecular cloning and characterization of winter flounder antifreeze
RT   cDNA.";
RL   Proc. Natl. Acad. Sci. U.S.A. 78:2825-2829(1981).
CC   -!- FUNCTION: Contributes to protect fish blood from freezing at subzero
CC       sea water temperatures. Lowers the blood freezing point. Binds to
CC       nascent ice crystals and prevents further growth (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the type-I AFP family. {ECO:0000305}.
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DR   EMBL; J00930; AAA49467.1; -; mRNA.
DR   PIR; A03193; FDFL4W.
DR   AlphaFoldDB; P02734; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050825; F:ice binding; IEA:InterPro.
DR   InterPro; IPR000104; Antifreeze_1.
DR   PRINTS; PR00308; ANTIFREEZEI.
PE   3: Inferred from homology;
KW   Antifreeze protein; Repeat; Secreted; Signal.
FT   SIGNAL          1..21
FT   CHAIN           22..85
FT                   /note="Ice-structuring protein 4"
FT                   /id="PRO_0000001684"
SQ   SEQUENCE   85 AA;  7215 MW;  8E62E1D2B44117BC CRC64;
     MRITEANPDP DAKAVPAAAA PSTASDAAAA AAATAATAAA AAAATAATAA AAAAATAATA
     AKAAALTAAN AAAAAAATAA AAARG