ID   ANP4_ZOAAM              Reviewed;          63 AA.
AC   P19609;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Ice-structuring protein SP1(HPLC 4);
DE            Short=ISP SP1(HPLC 4);
DE   AltName: Full=Antifreeze protein SP1(HPLC 4);
OS   Zoarces americanus (Ocean pout) (Macrozoarces americanus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Perciformes; Cottioidei; Zoarcales; Zoarcidae; Zoarcinae;
OC   Zoarces.
OX   NCBI_TaxID=8199;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=3403560; DOI=10.1016/s0021-9258(18)37891-8;
RA   Hew C.-L., Wang N.-C., Joshi S., Fletcher G.L., Scott G.K., Hayes P.H.,
RA   Buettner B., Davies P.L.;
RT   "Multiple genes provide the basis for antifreeze protein diversity and
RT   dosage in the ocean pout, Macrozoarces americanus.";
RL   J. Biol. Chem. 263:12049-12055(1988).
CC   -!- FUNCTION: Contributes to protect fish blood from freezing at subzero
CC       sea water temperatures. Lowers the blood freezing point. Binds to
CC       nascent ice crystals and prevents further growth (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:3403560}.
CC   -!- TISSUE SPECIFICITY: Detected in blood serum (at protein level).
CC       {ECO:0000269|PubMed:3403560}.
CC   -!- SIMILARITY: Belongs to the type-III AFP family. {ECO:0000305}.
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DR   PIR; E31075; E31075.
DR   AlphaFoldDB; P19609; -.
DR   SMR; P19609; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR006190; AFP_Neu5c_C.
DR   InterPro; IPR036732; AFP_Neu5c_C_sf.
DR   InterPro; IPR006013; Antifreeze_III.
DR   InterPro; IPR013974; SAF.
DR   Pfam; PF08666; SAF; 1.
DR   PRINTS; PR00357; ANTIFREEZIII.
DR   SMART; SM00858; SAF; 1.
DR   SUPFAM; SSF51269; SSF51269; 1.
DR   PROSITE; PS50844; AFP_LIKE; 1.
PE   1: Evidence at protein level;
KW   Antifreeze protein; Direct protein sequencing; Secreted.
FT   CHAIN           1..63
FT                   /note="Ice-structuring protein SP1(HPLC 4)"
FT                   /id="PRO_0000155157"
FT   DOMAIN          2..61
FT                   /note="AFP-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00021"
FT   SITE            7
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            12
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            16
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            42
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   63 AA;  6642 MW;  3FEFE507C6972FD8 CRC64;
     QSVVATQLIP INTALTPAMM EGKVTNPIGI PFAEMSQIVG KQVNTPVAKG QTIMPNMVKT
     YAA