HIS61_PSEAE
ID HIS61_PSEAE Reviewed; 256 AA.
AC Q9HU44;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Imidazole glycerol phosphate synthase subunit hisF1;
DE EC=4.3.2.10;
DE AltName: Full=IGP synthase cyclase subunit;
DE AltName: Full=IGP synthase subunit hisF1;
DE AltName: Full=ImGP synthase subunit hisF1;
DE Short=IGPS subunit hisF1;
GN Name=hisF1; OrderedLocusNames=PA5140;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: IGPS catalyzes the conversion of PRFAR and glutamine to IGP,
CC AICAR and glutamate. The HisF subunit catalyzes the cyclization
CC activity that produces IGP and AICAR from PRFAR using the ammonia
CC provided by the HisH subunit (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-
CC phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-
CC amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-
CC erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate;
CC Xref=Rhea:RHEA:24793, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985,
CC ChEBI:CHEBI:58278, ChEBI:CHEBI:58359, ChEBI:CHEBI:58475,
CC ChEBI:CHEBI:58525; EC=4.3.2.10;
CC -!- PATHWAY: Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine
CC from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.
CC -!- SUBUNIT: Heterodimer of HisH and HisF. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the HisA/HisF family. {ECO:0000305}.
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DR EMBL; AE004091; AAG08525.1; -; Genomic_DNA.
DR PIR; H83002; H83002.
DR RefSeq; NP_253827.1; NC_002516.2.
DR RefSeq; WP_003106334.1; NZ_QZGE01000002.1.
DR AlphaFoldDB; Q9HU44; -.
DR SMR; Q9HU44; -.
DR STRING; 287.DR97_2494; -.
DR PaxDb; Q9HU44; -.
DR PRIDE; Q9HU44; -.
DR EnsemblBacteria; AAG08525; AAG08525; PA5140.
DR GeneID; 878541; -.
DR KEGG; pae:PA5140; -.
DR PATRIC; fig|208964.12.peg.5386; -.
DR PseudoCAP; PA5140; -.
DR HOGENOM; CLU_048577_4_0_6; -.
DR InParanoid; Q9HU44; -.
DR OMA; IMPCLDM; -.
DR PhylomeDB; Q9HU44; -.
DR BioCyc; PAER208964:G1FZ6-5255-MON; -.
DR UniPathway; UPA00031; UER00010.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000107; F:imidazoleglycerol-phosphate synthase activity; IBA:GO_Central.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0000105; P:histidine biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd04731; HisF; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR HAMAP; MF_01013; HisF; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR006062; His_biosynth.
DR InterPro; IPR004651; HisF.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR Pfam; PF00977; His_biosynth; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
DR TIGRFAMs; TIGR00735; hisF; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Cytoplasm; Histidine biosynthesis; Lyase;
KW Reference proteome.
FT CHAIN 1..256
FT /note="Imidazole glycerol phosphate synthase subunit hisF1"
FT /id="PRO_0000142206"
FT ACT_SITE 12
FT /evidence="ECO:0000255"
FT ACT_SITE 131
FT /evidence="ECO:0000255"
SQ SEQUENCE 256 AA; 27131 MW; 6486A76CD308FAA7 CRC64;
MALAKRIIPC LDVDNGRVVK GVKFENIRDA GDPVEIARRY DEQGADEITF LDITASVDGR
DTTLHTVERM ASQVFIPLTV GGGVRSVQDI RNLLNAGADK VSINTAAVFN PEFVGEAADR
FGSQCIVVAI DAKKVSAPGE APRWEIFTHG GRKPTGLDAV LWAKKMEDLG AGEILLTSMD
QDGVKSGYDL GVTRAISEAV NVPVIASGGV GNLEHLAAGI LEGKADAVLA ASIFHFGEYT
VPEAKAYLAS RGIVVR
