ID   ANP5_ZOAAM              Reviewed;          87 AA.
AC   P19607;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Ice-structuring protein lambda OP-5;
DE            Short=ISP lambda OP-5;
DE   AltName: Full=Antifreeze protein lambda OP-5;
DE   Flags: Precursor;
OS   Zoarces americanus (Ocean pout) (Macrozoarces americanus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Perciformes; Cottioidei; Zoarcales; Zoarcidae; Zoarcinae;
OC   Zoarces.
OX   NCBI_TaxID=8199;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3403560; DOI=10.1016/s0021-9258(18)37891-8;
RA   Hew C.-L., Wang N.-C., Joshi S., Fletcher G.L., Scott G.K., Hayes P.H.,
RA   Buettner B., Davies P.L.;
RT   "Multiple genes provide the basis for antifreeze protein diversity and
RT   dosage in the ocean pout, Macrozoarces americanus.";
RL   J. Biol. Chem. 263:12049-12055(1988).
CC   -!- FUNCTION: Contributes to protect fish blood from freezing at subzero
CC       sea water temperatures. Lowers the blood freezing point. Binds to
CC       nascent ice crystals and prevents further growth (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the type-III AFP family. {ECO:0000305}.
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DR   EMBL; J03924; AAA49346.1; -; Genomic_DNA.
DR   PIR; C31075; C31075.
DR   AlphaFoldDB; P19607; -.
DR   SMR; P19607; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR006190; AFP_Neu5c_C.
DR   InterPro; IPR036732; AFP_Neu5c_C_sf.
DR   InterPro; IPR006013; Antifreeze_III.
DR   InterPro; IPR013974; SAF.
DR   Pfam; PF08666; SAF; 1.
DR   PRINTS; PR00357; ANTIFREEZIII.
DR   SMART; SM00858; SAF; 1.
DR   SUPFAM; SSF51269; SSF51269; 1.
DR   PROSITE; PS50844; AFP_LIKE; 1.
PE   3: Inferred from homology;
KW   Antifreeze protein; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000250"
FT   CHAIN           22..87
FT                   /note="Ice-structuring protein lambda OP-5"
FT                   /id="PRO_0000001695"
FT   DOMAIN          24..83
FT                   /note="AFP-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00021"
FT   SITE            29
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            34
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            38
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            64
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   87 AA;  9229 MW;  B5615B2E868D87AB CRC64;
     MKSVILTGLL FVLLCVDHMT ASQSVVATQL IPINTALTPV MMEGKVTNPI GIPFAEMSQI
     VGKQVNTPVA KGQTLMPNMV KTYAAGK