HIS62_PSEAE
ID HIS62_PSEAE Reviewed; 251 AA.
AC P72139; Q8KIS2;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Putative imidazole glycerol phosphate synthase subunit hisF2;
DE EC=4.3.2.10;
DE AltName: Full=IGP synthase cyclase subunit;
DE AltName: Full=IGP synthase subunit hisF2;
DE AltName: Full=ImGP synthase subunit hisF2;
DE Short=IGPS subunit hisF2;
GN Name=hisF2; OrderedLocusNames=PA3151;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8939432; DOI=10.1046/j.1365-2958.1996.1351503.x;
RA Burrows L.L., Charter D.F., Lam J.S.;
RT "Molecular characterization of the Pseudomonas aeruginosa serotype O5
RT (PAO1) B-band lipopolysaccharide gene cluster.";
RL Mol. Microbiol. 22:481-495(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Serotype O16, Serotype O18, Serotype O2, Serotype O20, and
RC Serotype O5;
RX PubMed=12057956; DOI=10.1128/jb.184.13.3614-3622.2002;
RA Raymond C.K., Sims E.H., Kas A., Spencer D.H., Kutyavin T.V., Ivey R.G.,
RA Zhou Y., Kaul R., Clendenning J.B., Olson M.V.;
RT "Genetic variation at the O-antigen biosynthetic locus in Pseudomonas
RT aeruginosa.";
RL J. Bacteriol. 184:3614-3622(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: IGPS catalyzes the conversion of PRFAR and glutamine to IGP,
CC AICAR and glutamate. The HisF subunit catalyzes the cyclization
CC activity that produces IGP and AICAR from PRFAR using the ammonia
CC provided by the HisH subunit (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-
CC phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-
CC amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-
CC erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate;
CC Xref=Rhea:RHEA:24793, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985,
CC ChEBI:CHEBI:58278, ChEBI:CHEBI:58359, ChEBI:CHEBI:58475,
CC ChEBI:CHEBI:58525; EC=4.3.2.10;
CC -!- PATHWAY: Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine
CC from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.
CC -!- SUBUNIT: Heterodimer of HisH and HisF. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the HisA/HisF family. {ECO:0000305}.
CC -!- CAUTION: The potential active site Asp residue in position 11 is
CC replaced by a Leu. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U50396; AAC45860.1; -; Genomic_DNA.
DR EMBL; AF498408; AAM27665.1; -; Genomic_DNA.
DR EMBL; AF498410; AAM27696.1; -; Genomic_DNA.
DR EMBL; AF498412; AAM27731.1; -; Genomic_DNA.
DR EMBL; AF498413; AAM27751.1; -; Genomic_DNA.
DR EMBL; AF498416; AAM27804.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG06539.1; -; Genomic_DNA.
DR PIR; A83251; A83251.
DR RefSeq; NP_251841.1; NC_002516.2.
DR RefSeq; WP_003113421.1; NZ_QZGE01000023.1.
DR AlphaFoldDB; P72139; -.
DR SMR; P72139; -.
DR STRING; 208964.PA3151; -.
DR PaxDb; P72139; -.
DR PRIDE; P72139; -.
DR DNASU; 882582; -.
DR EnsemblBacteria; AAG06539; AAG06539; PA3151.
DR GeneID; 882582; -.
DR KEGG; pae:PA3151; -.
DR PATRIC; fig|208964.12.peg.3298; -.
DR PseudoCAP; PA3151; -.
DR HOGENOM; CLU_048577_4_0_6; -.
DR InParanoid; P72139; -.
DR OMA; PKYVGDP; -.
DR PhylomeDB; P72139; -.
DR BioCyc; PAER208964:G1FZ6-3211-MON; -.
DR UniPathway; UPA00031; UER00010.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000107; F:imidazoleglycerol-phosphate synthase activity; IBA:GO_Central.
DR GO; GO:0016833; F:oxo-acid-lyase activity; IEA:InterPro.
DR GO; GO:0000105; P:histidine biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0009243; P:O antigen biosynthetic process; EXP:PseudoCAP.
DR CDD; cd04731; HisF; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR020021; Glycosyl_amidation-assoc_WbuZ.
DR InterPro; IPR006062; His_biosynth.
DR InterPro; IPR004651; HisF.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR Pfam; PF00977; His_biosynth; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
DR TIGRFAMs; TIGR03572; WbuZ; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Cytoplasm; Histidine biosynthesis; Lyase;
KW Reference proteome.
FT CHAIN 1..251
FT /note="Putative imidazole glycerol phosphate synthase
FT subunit hisF2"
FT /id="PRO_0000142207"
FT ACT_SITE 130
FT /evidence="ECO:0000255"
FT VARIANT 22
FT /note="K -> R (in strain: Serotype O16)"
FT VARIANT 110
FT /note="L -> I (in strain: Serotype O16)"
FT VARIANT 134
FT /note="G -> S (in strain: Serotype O16)"
SQ SEQUENCE 251 AA; 27449 MW; 6B1F48BFC9D551BD CRC64;
MVRRRVIPCL LLKDRGLVKT VKFKEPKYVG DPINAIRIFN EKEVDELILL DIDASRLNQE
PNYELIAEVA GECFMPICYG GGIKTLEHAE KIFSLGVEKV SINTAALMDL SLIRRIADKF
GSQSVVGSID CRKGFWGGHS VFSENGTRDM KRSPLEWAQA LEEAGVGEIF LNSIDRDGVQ
KGFDNALVEN IASNVHVPVI ACGGAGSIAD LIDLFERTCV SAVAAGSLFV FHGKHRAVLI
SYPDVNKLDV G
