ID   ANP9_ZOAAM              Reviewed;          64 AA.
AC   P19612;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Ice-structuring protein SP3(HPLC 9);
DE            Short=ISP SP3(HPLC 9);
DE   AltName: Full=Antifreeze protein SP3(HPLC 9);
OS   Zoarces americanus (Ocean pout) (Macrozoarces americanus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Perciformes; Cottioidei; Zoarcales; Zoarcidae; Zoarcinae;
OC   Zoarces.
OX   NCBI_TaxID=8199;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=3403560; DOI=10.1016/s0021-9258(18)37891-8;
RA   Hew C.-L., Wang N.-C., Joshi S., Fletcher G.L., Scott G.K., Hayes P.H.,
RA   Buettner B., Davies P.L.;
RT   "Multiple genes provide the basis for antifreeze protein diversity and
RT   dosage in the ocean pout, Macrozoarces americanus.";
RL   J. Biol. Chem. 263:12049-12055(1988).
CC   -!- FUNCTION: Contributes to protect fish blood from freezing at subzero
CC       sea water temperatures. Lowers the blood freezing point. Binds to
CC       nascent ice crystals and prevents further growth (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:3403560}.
CC   -!- TISSUE SPECIFICITY: Detected in blood serum (at protein level).
CC       {ECO:0000269|PubMed:3403560}.
CC   -!- SIMILARITY: Belongs to the type-III AFP family. {ECO:0000305}.
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DR   PIR; H31075; H31075.
DR   AlphaFoldDB; P19612; -.
DR   SMR; P19612; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR006190; AFP_Neu5c_C.
DR   InterPro; IPR036732; AFP_Neu5c_C_sf.
DR   InterPro; IPR006013; Antifreeze_III.
DR   InterPro; IPR013974; SAF.
DR   Pfam; PF08666; SAF; 1.
DR   PRINTS; PR00357; ANTIFREEZIII.
DR   SMART; SM00858; SAF; 1.
DR   SUPFAM; SSF51269; SSF51269; 1.
DR   PROSITE; PS50844; AFP_LIKE; 1.
PE   1: Evidence at protein level;
KW   Antifreeze protein; Direct protein sequencing; Secreted.
FT   CHAIN           1..64
FT                   /note="Ice-structuring protein SP3(HPLC 9)"
FT                   /id="PRO_0000155159"
FT   DOMAIN          3..62
FT                   /note="AFP-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00021"
FT   SITE            8
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            13
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            17
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            43
FT                   /note="Important for ice-binding"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   64 AA;  6818 MW;  FE2D27D8318C423A CRC64;
     SQSVVATQLI PMNTALTPAM MEGKVTNPIG IPFAEMSQIV GKQVNRIVAK GQTLMPNMVK
     TYAA