ID   ANP_MYZFE               Reviewed;          97 AA.
AC   P09031;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Ice-structuring protein;
DE            Short=ISP;
DE   AltName: Full=Antifreeze protein;
DE            Short=AFP;
DE   Flags: Precursor;
OS   Myzopsetta ferruginea (Yellowtail flounder) (Platessa ferruginea).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Carangaria; Pleuronectiformes; Pleuronectoidei; Pleuronectidae; Myzopsetta.
OX   NCBI_TaxID=8258;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR
RP   LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=3665937; DOI=10.1111/j.1432-1033.1987.tb13462.x;
RA   Scott G.K., Davies P.L., Shears M.A., Fletcher G.L.;
RT   "Structural variations in the alanine-rich antifreeze proteins of the
RT   pleuronectinae.";
RL   Eur. J. Biochem. 168:629-633(1987).
CC   -!- FUNCTION: Contributes to protect fish blood from freezing at subzero
CC       sea water temperatures. Lowers the blood freezing point. Binds to
CC       nascent ice crystals and prevents further growth.
CC       {ECO:0000269|PubMed:3665937}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:3665937}.
CC   -!- TISSUE SPECIFICITY: Detected in blood serum (at protein level).
CC       {ECO:0000269|PubMed:3665937}.
CC   -!- SIMILARITY: Belongs to the type-I AFP family. {ECO:0000305}.
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DR   EMBL; X06356; CAA29655.1; -; mRNA.
DR   PIR; S02376; S02376.
DR   AlphaFoldDB; P09031; -.
DR   SMR; P09031; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050825; F:ice binding; IEA:InterPro.
DR   InterPro; IPR000104; Antifreeze_1.
DR   PRINTS; PR00308; ANTIFREEZEI.
PE   1: Evidence at protein level;
KW   Antifreeze protein; Direct protein sequencing; Repeat; Secreted; Signal.
FT   SIGNAL          1..23
FT   PROPEP          24..48
FT                   /note="Removed by a dipeptidylpeptidase"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000001682"
FT   CHAIN           49..97
FT                   /note="Ice-structuring protein"
FT                   /id="PRO_0000001683"
SQ   SEQUENCE   97 AA;  8865 MW;  62AD582DF8E459B6 CRC64;
     MALSLFTVGQ LIFLFWTLRI TEANPDPAAK AAPAAVADPA AAAAAAVADT ASDAAAAAAA
     TAAAAAKAAA DTAAAAAKAA ADTAAAAAEA AAATARG