ANP_NOTNE
ID ANP_NOTNE Reviewed; 790 AA.
AC P24856;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=Ice-structuring glycoprotein;
DE Short=ISGP;
DE AltName: Full=Antifreeze glycopeptide polyprotein;
DE Short=AFGP polyprotein;
DE Contains:
DE RecName: Full=AFGP7-1;
DE AltName: Full=AFGP 7;
DE Contains:
DE RecName: Full=AFGP7-2;
DE AltName: Full=AFGP 7;
DE Contains:
DE RecName: Full=AFGP8-1;
DE AltName: Full=AFGP 8;
DE Contains:
DE RecName: Full=AFGP8-2;
DE AltName: Full=AFGP 8;
DE Contains:
DE RecName: Full=AFGP8-3;
DE AltName: Full=AFGP 8;
DE Contains:
DE RecName: Full=AFGP8-4;
DE AltName: Full=AFGP 8;
DE Contains:
DE RecName: Full=AFGP8-5;
DE AltName: Full=AFGP 8;
DE Contains:
DE RecName: Full=AFGP8-like;
DE Flags: Precursor; Fragment;
GN Name=afgp8;
OS Notothenia neglecta (Yellowbelly rockcod) (Notothenia coriiceps neglecta).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Perciformes; Notothenioidei; Nototheniidae; Notothenia.
OX NCBI_TaxID=202063;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC TISSUE=Testis;
RX PubMed=2251271; DOI=10.1073/pnas.87.23.9265;
RA Hsiao K.-C., Cheng C.-H.C., Fernandes I.E., Detrich H.W. III, Devries A.L.;
RT "An antifreeze glycopeptide gene from the antarctic cod Notothenia
RT coriiceps neglecta encodes a polyprotein of high peptide copy number.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:9265-9269(1990).
RN [2]
RP SEQUENCE REVISION TO N-TERMINUS AND 457.
RA Cheng C.-H.C.;
RL Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Antifreeze proteins lower the blood freezing point.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Synthesized by the liver and secreted into the
CC blood from which they become distributed to almost the entire
CC extracellular space.
CC -!- DOMAIN: Contains 44 copies of AFGP8 and two copies of AFGP7.
CC -!- PTM: O-glycosylated; contains disaccharide galactose-N-
CC acetylgalactosamine attached to threonines in AFGP8 and AFGP7.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=Cool news - Issue 5 of
CC December 2000;
CC URL="https://web.expasy.org/spotlight/back_issues/005";
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DR EMBL; M55000; AAA49392.2; -; Genomic_DNA.
DR PIR; A38420; A38420.
DR AlphaFoldDB; P24856; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Antifreeze protein; Direct protein sequencing; Glycoprotein; Repeat;
KW Secreted.
FT PROPEP <1..5
FT /id="PRO_0000001634"
FT CHAIN 6..790
FT /note="Ice-structuring glycoprotein"
FT /id="PRO_0000001635"
FT PEPTIDE 6..19
FT /note="AFGP8-1"
FT /id="PRO_0000001636"
FT PEPTIDE 23..36
FT /note="AFGP8-5"
FT /id="PRO_0000001637"
FT PEPTIDE 40..53
FT /note="AFGP8-1"
FT /id="PRO_0000001638"
FT PEPTIDE 57..70
FT /note="AFGP8-4"
FT /id="PRO_0000001639"
FT PEPTIDE 74..87
FT /note="AFGP8-2"
FT /id="PRO_0000001640"
FT PEPTIDE 91..104
FT /note="AFGP8-4"
FT /id="PRO_0000001641"
FT PEPTIDE 108..121
FT /note="AFGP8-3"
FT /id="PRO_0000001642"
FT PEPTIDE 125..138
FT /note="AFGP8-4"
FT /id="PRO_0000001643"
FT PEPTIDE 142..155
FT /note="AFGP8-3"
FT /id="PRO_0000001644"
FT PEPTIDE 159..172
FT /note="AFGP8-4"
FT /id="PRO_0000001645"
FT PEPTIDE 176..189
FT /note="AFGP8-1"
FT /id="PRO_0000001646"
FT PEPTIDE 193..206
FT /note="AFGP8-4"
FT /id="PRO_0000001647"
FT PEPTIDE 210..223
FT /note="AFGP8-1"
FT /id="PRO_0000001648"
FT PEPTIDE 227..240
FT /note="AFGP8-1"
FT /id="PRO_0000001649"
FT PEPTIDE 244..257
FT /note="AFGP8-4"
FT /id="PRO_0000001650"
FT PEPTIDE 261..274
FT /note="AFGP8-1"
FT /id="PRO_0000001651"
FT PEPTIDE 278..291
FT /note="AFGP8-4"
FT /id="PRO_0000001652"
FT PEPTIDE 295..308
FT /note="AFGP8-1"
FT /id="PRO_0000001653"
FT PEPTIDE 312..325
FT /note="AFGP8-4"
FT /id="PRO_0000001654"
FT PEPTIDE 329..342
FT /note="AFGP8-1"
FT /id="PRO_0000001655"
FT PEPTIDE 346..359
FT /note="AFGP8-4"
FT /id="PRO_0000001656"
FT PEPTIDE 363..376
FT /note="AFGP8-1"
FT /id="PRO_0000001657"
FT PEPTIDE 380..393
FT /note="AFGP8-3"
FT /id="PRO_0000001658"
FT PEPTIDE 397..410
FT /note="AFGP8-4"
FT /id="PRO_0000001659"
FT PEPTIDE 414..427
FT /note="AFGP8-1"
FT /id="PRO_0000001660"
FT PEPTIDE 431..444
FT /note="AFGP8-4"
FT /id="PRO_0000001661"
FT PEPTIDE 448..461
FT /note="AFGP8-1"
FT /id="PRO_0000001662"
FT PEPTIDE 465..478
FT /note="AFGP8-1"
FT /id="PRO_0000001663"
FT PEPTIDE 482..495
FT /note="AFGP8-4"
FT /id="PRO_0000001664"
FT PEPTIDE 499..512
FT /note="AFGP8-1"
FT /id="PRO_0000001665"
FT PEPTIDE 516..529
FT /note="AFGP8-1"
FT /id="PRO_0000001666"
FT PEPTIDE 533..546
FT /note="AFGP8-4"
FT /id="PRO_0000001667"
FT PEPTIDE 550..563
FT /note="AFGP8-1"
FT /id="PRO_0000001668"
FT PEPTIDE 567..580
FT /note="AFGP8-4"
FT /id="PRO_0000001669"
FT PEPTIDE 584..597
FT /note="AFGP8-3"
FT /id="PRO_0000001670"
FT PEPTIDE 601..614
FT /note="AFGP8-4"
FT /id="PRO_0000001671"
FT PEPTIDE 618..631
FT /note="AFGP8-1"
FT /id="PRO_0000001672"
FT PEPTIDE 635..648
FT /note="AFGP8-1"
FT /id="PRO_0000001673"
FT PEPTIDE 652..665
FT /note="AFGP8-4"
FT /id="PRO_0000001674"
FT PEPTIDE 669..682
FT /note="AFGP8-1"
FT /id="PRO_0000001675"
FT PEPTIDE 686..699
FT /note="AFGP8-4"
FT /id="PRO_0000001676"
FT PEPTIDE 703..716
FT /note="AFGP8-1"
FT /id="PRO_0000001677"
FT PEPTIDE 720..736
FT /note="AFGP7-1"
FT /id="PRO_0000001678"
FT PEPTIDE 740..756
FT /note="AFGP7-2"
FT /id="PRO_0000001679"
FT PEPTIDE 760..773
FT /note="AFGP8-3"
FT /id="PRO_0000001680"
FT PEPTIDE 777..790
FT /note="AFGP8-like"
FT /id="PRO_0000001681"
FT NON_TER 1
SQ SEQUENCE 790 AA; 71267 MW; 4C7CCCADC48FE902 CRC64;
VTAAPAATAA TAATPATAAL NFAATAATPA TPATPALIFA ATAATAATPA TAALNFAATA
ATPATAATPA LIFAAAAATA ATPATAALNF AATAATPATA ATPALIFAAT AATAATPATP
AFHFAATAAT PATAATPALI FAATAATAAT PATPAFHFAA TAATPATAAT PALIFAATAA
TAATPATAAL NFAATAATPA TAATPALIFA ATAATAATPA TAALNFAATA ATAATPATAA
CNFAATAATP ATAATPALIF AATAATAATP ATAACNFAAT AATPATAATP ALIFAATAAT
AATPATAALN FAATAATPAT AATPALIFAA TAATAATPAT AALNFAATAA TPATAATPAL
IFAATAATAA TPATAALNFA ATAATAATPA TPAFNFAATA ATPATAATPA LIFAATAATA
ATPATAALNF AATAATPATA ATPALIFAAT AATAATPATA ALHFAATAAT AATPATAALN
FAATAATPAT AATPALIFAA TAATAATPAT AAFNFAATAA TAATPATAAL NFAATAATPA
TAATPALIFA ATAATAATPA TAALNFAATA ATPATAATPA LIFAATAATA ATPATPAFHF
AATAATPATA ATPALIFAAT AATAATPATA ALNFAATAAT AATPATAALN FAATAATPAT
AATPALIFAA TAATAATPAT AALNFAATAA TPATAATPAL IFAATAATAA TPATAAFNFA
ATAATAATPA TAATPALIFA ATAATAATPA TPATPALIFA ATAATAATPA TPALNFAATA
ATAATTAARG
