ANR16_HUMAN
ID ANR16_HUMAN Reviewed; 361 AA.
AC Q6P6B7; A6NEF0; F8WEI4; Q9NT01;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Ankyrin repeat domain-containing protein 16 {ECO:0000305};
GN Name=ANKRD16 {ECO:0000312|HGNC:HGNC:23471};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 202-361 (ISOFORM 1), AND VARIANT
RP ARG-353.
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
CC -!- FUNCTION: Required to prevent the misactivation of serine (Ser) with
CC tRNA(Ala) by promoting the hydrolysis of Ser-mischarged tRNA(Ala),
CC thereby playing a role in translational fidelity. Binds directly to the
CC catalytic domain of AARS/AlaRS and captures Ser that is misactivated by
CC AARS/AlaRS, preventing the charging of Ser adenylates to tRNA(Ala) and
CC precluding Ser misincorporation in nascent peptides.
CC {ECO:0000250|UniProtKB:A2AS55}.
CC -!- SUBUNIT: Interacts with AARS; the interaction is direct.
CC {ECO:0000250|UniProtKB:A2AS55}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:A2AS55}. Nucleus
CC {ECO:0000250|UniProtKB:A2AS55}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6P6B7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6P6B7-2; Sequence=VSP_047046, VSP_047047;
CC -!- DOMAIN: Side chains of Lys-102, Lys-135 and Lys-165 capture Ser that is
CC misactivated by AARS/AlaRS. {ECO:0000250|UniProtKB:A2AS55}.
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DR EMBL; AL137186; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL596094; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC062346; AAH62346.1; -; mRNA.
DR EMBL; AL137614; CAB70843.1; -; mRNA.
DR CCDS; CCDS31136.1; -. [Q6P6B7-1]
DR CCDS; CCDS31137.1; -. [Q6P6B7-2]
DR PIR; T46316; T46316.
DR RefSeq; NP_001009941.1; NM_001009941.2. [Q6P6B7-1]
DR RefSeq; NP_001009943.1; NM_001009943.2. [Q6P6B7-2]
DR RefSeq; NP_061919.1; NM_019046.2. [Q6P6B7-1]
DR AlphaFoldDB; Q6P6B7; -.
DR SMR; Q6P6B7; -.
DR BioGRID; 120015; 6.
DR IntAct; Q6P6B7; 3.
DR STRING; 9606.ENSP00000369436; -.
DR iPTMnet; Q6P6B7; -.
DR PhosphoSitePlus; Q6P6B7; -.
DR BioMuta; ANKRD16; -.
DR DMDM; 74749136; -.
DR EPD; Q6P6B7; -.
DR jPOST; Q6P6B7; -.
DR MassIVE; Q6P6B7; -.
DR MaxQB; Q6P6B7; -.
DR PaxDb; Q6P6B7; -.
DR PeptideAtlas; Q6P6B7; -.
DR PRIDE; Q6P6B7; -.
DR ProteomicsDB; 31852; -.
DR ProteomicsDB; 67019; -. [Q6P6B7-1]
DR Antibodypedia; 51456; 34 antibodies from 15 providers.
DR DNASU; 54522; -.
DR Ensembl; ENST00000191063.8; ENSP00000352361.6; ENSG00000134461.16. [Q6P6B7-2]
DR Ensembl; ENST00000380092.8; ENSP00000369434.4; ENSG00000134461.16. [Q6P6B7-1]
DR Ensembl; ENST00000380094.10; ENSP00000369436.4; ENSG00000134461.16. [Q6P6B7-1]
DR GeneID; 54522; -.
DR KEGG; hsa:54522; -.
DR MANE-Select; ENST00000380094.10; ENSP00000369436.4; NM_019046.3; NP_061919.1.
DR UCSC; uc001iiq.4; human. [Q6P6B7-1]
DR CTD; 54522; -.
DR DisGeNET; 54522; -.
DR GeneCards; ANKRD16; -.
DR HGNC; HGNC:23471; ANKRD16.
DR HPA; ENSG00000134461; Low tissue specificity.
DR MIM; 618017; gene.
DR neXtProt; NX_Q6P6B7; -.
DR OpenTargets; ENSG00000134461; -.
DR PharmGKB; PA134916467; -.
DR VEuPathDB; HostDB:ENSG00000134461; -.
DR eggNOG; KOG0504; Eukaryota.
DR GeneTree; ENSGT00940000153969; -.
DR HOGENOM; CLU_000134_48_2_1; -.
DR InParanoid; Q6P6B7; -.
DR OMA; QYEPDCK; -.
DR OrthoDB; 1073161at2759; -.
DR PhylomeDB; Q6P6B7; -.
DR TreeFam; TF329520; -.
DR PathwayCommons; Q6P6B7; -.
DR SignaLink; Q6P6B7; -.
DR BioGRID-ORCS; 54522; 10 hits in 1077 CRISPR screens.
DR ChiTaRS; ANKRD16; human.
DR GenomeRNAi; 54522; -.
DR Pharos; Q6P6B7; Tdark.
DR PRO; PR:Q6P6B7; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q6P6B7; protein.
DR Bgee; ENSG00000134461; Expressed in ectocervix and 134 other tissues.
DR Genevisible; Q6P6B7; HS.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0006400; P:tRNA modification; ISS:UniProtKB.
DR Gene3D; 1.25.40.20; -; 3.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR Pfam; PF00023; Ank; 1.
DR Pfam; PF12796; Ank_2; 2.
DR Pfam; PF13637; Ank_4; 1.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 9.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 5.
PE 1: Evidence at protein level;
KW Alternative splicing; ANK repeat; Cytoplasm; Nucleus; Reference proteome;
KW Repeat.
FT CHAIN 1..361
FT /note="Ankyrin repeat domain-containing protein 16"
FT /id="PRO_0000240831"
FT REPEAT 36..66
FT /note="ANK 1"
FT REPEAT 70..99
FT /note="ANK 2"
FT REPEAT 103..132
FT /note="ANK 3"
FT REPEAT 136..165
FT /note="ANK 4"
FT REPEAT 170..200
FT /note="ANK 5"
FT REPEAT 204..234
FT /note="ANK 6"
FT REPEAT 238..268
FT /note="ANK 7"
FT REPEAT 273..302
FT /note="ANK 8"
FT REPEAT 306..335
FT /note="ANK 9"
FT SITE 102
FT /note="Required to capture Ser that is misactivated by
FT AARS/AlaRS"
FT /evidence="ECO:0000250|UniProtKB:A2AS55"
FT SITE 135
FT /note="Required to capture Ser that is misactivated by
FT AARS/AlaRS"
FT /evidence="ECO:0000250|UniProtKB:A2AS55"
FT SITE 165
FT /note="Required to capture Ser that is misactivated by
FT AARS/AlaRS"
FT /evidence="ECO:0000250|UniProtKB:A2AS55"
FT VAR_SEQ 284..304
FT /note="EGHTSTIQTLLSLGADINSKD -> PCIWPVQVSTWPVPSFSCSRD (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_047046"
FT VAR_SEQ 305..361
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_047047"
FT VARIANT 128
FT /note="A -> G (in dbSNP:rs2296136)"
FT /id="VAR_033503"
FT VARIANT 353
FT /note="Q -> R (in dbSNP:rs1052420)"
FT /evidence="ECO:0000269|PubMed:17974005"
FT /id="VAR_026832"
SQ SEQUENCE 361 AA; 39284 MW; 825E2D6EC6225F06 CRC64;
MAQPGDPRRL CRLVQEGRLR ALKEELQAAG GCPGPAGDTL LHCAARHGHR DVLAYLAEAW
GMDIEATNRD YKRPLHEAAS MGHRDCVRYL LGRGAAVDCL KKADWTPLMM ACTRKNLGVI
QELVEHGANP LLKNKDGWNS FHIASREGDP LILQYLLTVC PGAWKTESKI RRTPLHTAAM
HGHLEAVKVL LKRCQYEPDY RDNCGVTALM DAIQCGHIDV ARLLLDEHGA CLSAEDSLGA
QALHRAAVTG QDEAIRFLVS ELGVDVDVRA TSTHLTALHY AAKEGHTSTI QTLLSLGADI
NSKDEKNRSA LHLACAGQHL ACAKFLLQSG LKDSEDITGT LAQQLPRRAD VLQGSGHSAM
T
