ID   ANR16_RAT               Reviewed;         370 AA.
AC   Q499M5;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Ankyrin repeat domain-containing protein 16;
GN   Name=Ankrd16;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Prostate;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Required to prevent the misactivation of serine (Ser) with
CC       tRNA(Ala) by promoting the hydrolysis of Ser-mischarged tRNA(Ala),
CC       thereby playing a role in translational fidelity. Binds directly to the
CC       catalytic domain of AARS/AlaRS and captures Ser that is misactivated by
CC       AARS/AlaRS, preventing the charging of Ser adenylates to tRNA(Ala) and
CC       precluding Ser misincorporation in nascent peptides.
CC       {ECO:0000250|UniProtKB:A2AS55}.
CC   -!- SUBUNIT: Interacts with AARS; the interaction is direct.
CC       {ECO:0000250|UniProtKB:A2AS55}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:A2AS55}. Nucleus
CC       {ECO:0000250|UniProtKB:A2AS55}.
CC   -!- DOMAIN: Side chains of Lys-111, Lys-144 and Lys-174 capture Ser that is
CC       misactivated by AARS/AlaRS. {ECO:0000250|UniProtKB:A2AS55}.
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DR   EMBL; BC099837; AAH99837.1; -; mRNA.
DR   RefSeq; NP_001028870.1; NM_001033698.1.
DR   AlphaFoldDB; Q499M5; -.
DR   SMR; Q499M5; -.
DR   STRING; 10116.ENSRNOP00000025011; -.
DR   PaxDb; Q499M5; -.
DR   Ensembl; ENSRNOT00000025011; ENSRNOP00000025011; ENSRNOG00000028168.
DR   GeneID; 307102; -.
DR   KEGG; rno:307102; -.
DR   UCSC; RGD:1311405; rat.
DR   CTD; 54522; -.
DR   RGD; 1311405; Ankrd16.
DR   eggNOG; KOG0504; Eukaryota.
DR   GeneTree; ENSGT00940000153969; -.
DR   HOGENOM; CLU_000134_48_2_1; -.
DR   InParanoid; Q499M5; -.
DR   OMA; QYEPDCK; -.
DR   OrthoDB; 1073161at2759; -.
DR   PhylomeDB; Q499M5; -.
DR   PRO; PR:Q499M5; -.
DR   Proteomes; UP000002494; Chromosome 17.
DR   Bgee; ENSRNOG00000028168; Expressed in thymus and 19 other tissues.
DR   Genevisible; Q499M5; RN.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0006400; P:tRNA modification; ISS:UniProtKB.
DR   Gene3D; 1.25.40.20; -; 3.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   Pfam; PF00023; Ank; 1.
DR   Pfam; PF12796; Ank_2; 1.
DR   Pfam; PF13637; Ank_4; 2.
DR   PRINTS; PR01415; ANKYRIN.
DR   SMART; SM00248; ANK; 9.
DR   SUPFAM; SSF48403; SSF48403; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 1.
DR   PROSITE; PS50088; ANK_REPEAT; 3.
PE   2: Evidence at transcript level;
KW   ANK repeat; Cytoplasm; Nucleus; Reference proteome; Repeat.
FT   CHAIN           1..370
FT                   /note="Ankyrin repeat domain-containing protein 16"
FT                   /id="PRO_0000240832"
FT   REPEAT          45..75
FT                   /note="ANK 1"
FT   REPEAT          79..108
FT                   /note="ANK 2"
FT   REPEAT          112..141
FT                   /note="ANK 3"
FT   REPEAT          145..174
FT                   /note="ANK 4"
FT   REPEAT          179..209
FT                   /note="ANK 5"
FT   REPEAT          213..242
FT                   /note="ANK 6"
FT   REPEAT          247..277
FT                   /note="ANK 7"
FT   REPEAT          282..311
FT                   /note="ANK 8"
FT   REPEAT          315..344
FT                   /note="ANK 9"
FT   SITE            111
FT                   /note="Required to capture Ser that is misactivated by
FT                   AARS/AlaRS"
FT                   /evidence="ECO:0000250|UniProtKB:A2AS55"
FT   SITE            144
FT                   /note="Required to capture Ser that is misactivated by
FT                   AARS/AlaRS"
FT                   /evidence="ECO:0000250|UniProtKB:A2AS55"
FT   SITE            174
FT                   /note="Required to capture Ser that is misactivated by
FT                   AARS/AlaRS"
FT                   /evidence="ECO:0000250|UniProtKB:A2AS55"
SQ   SEQUENCE   370 AA;  40560 MW;  EFF510C923D3F6B7 CRC64;
     MAPPGDPRRL CRLVQEGQLR ALREELEVAG GCWDPEMFRG SQGPAGDTLL HFASRHGRQD
     ILAYLVEAWS MDIEAANRDY KRPLHEAASM GHRDCVRYLL GRGAVVDSLK KADWTPLMMA
     CTRKNLEVIQ DLVEHGANPL LKNKDGWNSF HIASREGDPV ILRYLLTVCP DVWKTESKIR
     RTPLHTAAMH GCFEAVQELL ERCHYEPDCR DNCGVTPFMD AIQCGHVSIA KLLLENHEAC
     SSATDSLGAQ ALHRAAVTGQ DEAIRFLVCG LGIDVDVRAK SSQLTALLYA AKEGQTSTVQ
     TLLSLGADIN STDERNRSAL HLACAGQHAA CARFLRQSGL KDSADLTGAL AQQLTRGVAI
     LQDFDPGVKS