ANR1_ARATH
ID ANR1_ARATH Reviewed; 234 AA.
AC Q9SI38; O49351;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 142.
DE RecName: Full=MADS-box transcription factor ANR1;
DE AltName: Full=Protein AGAMOUS-LIKE 44;
DE AltName: Full=Protein ARABIDOPSIS NITRATE REGULATED 1;
GN Name=ANR1; Synonyms=AGL44; OrderedLocusNames=At2g14210; ORFNames=F15N24.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION BY NITRATE, DISRUPTION
RP PHENOTYPE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. C24, and cv. Landsberg erecta;
RX PubMed=9430595; DOI=10.1126/science.279.5349.407;
RA Zhang H., Forde B.G.;
RT "An Arabidopsis MADS box gene that controls nutrient-induced changes in
RT root architecture.";
RL Science 279:407-409(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP REVIEW.
RX PubMed=10938795; DOI=10.1093/jxb/51.342.51;
RA Zhang H., Forde B.G.;
RT "Regulation of Arabidopsis root development by nitrate availability.";
RL J. Exp. Bot. 51:51-59(2000).
RN [7]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12837945; DOI=10.1105/tpc.011544;
RA Parenicova L., de Folter S., Kieffer M., Horner D.S., Favalli C.,
RA Busscher J., Cook H.E., Ingram R.M., Kater M.M., Davies B., Angenent G.C.,
RA Colombo L.;
RT "Molecular and phylogenetic analyses of the complete MADS-box transcription
RT factor family in Arabidopsis: new openings to the MADS world.";
RL Plant Cell 15:1538-1551(2003).
RN [8]
RP FUNCTION.
RX PubMed=15667327; DOI=10.1042/bst0330283;
RA Filleur S., Walch-Liu P., Gan Y., Forde B.G.;
RT "Nitrate and glutamate sensing by plant roots.";
RL Biochem. Soc. Trans. 33:283-286(2005).
RN [9]
RP INDUCTION BY NITROGEN DEPRIVATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=16021502; DOI=10.1007/s00425-005-0020-3;
RA Gan Y., Filleur S., Rahman A., Gotensparre S., Forde B.G.;
RT "Nutritional regulation of ANR1 and other root-expressed MADS-box genes in
RT Arabidopsis thaliana.";
RL Planta 222:730-742(2005).
RN [10]
RP INTERACTION WITH AGL16; AGL21 AND SOC1.
RX PubMed=15805477; DOI=10.1105/tpc.105.031831;
RA de Folter S., Immink R.G.H., Kieffer M., Parenicova L., Henz S.R.,
RA Weigel D., Busscher M., Kooiker M., Colombo L., Kater M.M., Davies B.,
RA Angenent G.C.;
RT "Comprehensive interaction map of the Arabidopsis MADS Box transcription
RT factors.";
RL Plant Cell 17:1424-1433(2005).
RN [11]
RP REVIEW.
RX PubMed=16339770; DOI=10.1093/aob/mcj601;
RA Walch-Liu P., Ivanov I.I., Filleur S., Gan Y., Remans T., Forde B.G.;
RT "Nitrogen regulation of root branching.";
RL Ann. Bot. 97:875-881(2006).
RN [12]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION BY NITRATE.
RX PubMed=17148611; DOI=10.1073/pnas.0605275103;
RA Remans T., Nacry P., Pervent M., Filleur S., Diatloff E., Mounier E.,
RA Tillard P., Forde B.G., Gojon A.;
RT "The Arabidopsis NRT1.1 transporter participates in the signaling pathway
RT triggering root colonization of nitrate-rich patches.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:19206-19211(2006).
RN [13]
RP REVIEW.
RX PubMed=17578866; DOI=10.1093/jxb/erm114;
RA Zhang H., Rong H., Pilbeam D.;
RT "Signalling mechanisms underlying the morphological responses of the root
RT system to nitrogen in Arabidopsis thaliana.";
RL J. Exp. Bot. 58:2329-2338(2007).
CC -!- FUNCTION: Probable transcription factor. Required for root plasticity
CC in response to nitrate, NO(3)(-). Promotes lateral root growth in a
CC NRT1.1-dependent manner. {ECO:0000269|PubMed:15667327,
CC ECO:0000269|PubMed:17148611, ECO:0000269|PubMed:9430595}.
CC -!- SUBUNIT: Interacts with AGL16, AGL21 and SOC1.
CC {ECO:0000269|PubMed:15805477}.
CC -!- INTERACTION:
CC Q9SI38; Q9SZJ6: AGL21; NbExp=4; IntAct=EBI-622096, EBI-621986;
CC Q9SI38; O80748: B-box domain protein 26; NbExp=3; IntAct=EBI-622096, EBI-15191535;
CC Q9SI38; Q0V7X4: FIT; NbExp=3; IntAct=EBI-622096, EBI-1640543;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in roots, mostly in lateral
CC roots (LR) primordia, young emerging LRs, apex and base of LRs, apex of
CC the primary root, and in the stele. Barely detectable in shoots.
CC {ECO:0000269|PubMed:12837945, ECO:0000269|PubMed:16021502,
CC ECO:0000269|PubMed:17148611, ECO:0000269|PubMed:9430595}.
CC -!- INDUCTION: Induced by nitrate in root cell culture, (PubMed:9430595,
CC PubMed:17148611). In roots, seems induced by nitrogen (N) deprivation
CC (e.g. nitrate free medium) but rapidly repressed by N re-supply (e.g.
CC nitrate, glutamine and ammonium) (PubMed:16021502). Slight repression
CC in shoots during nitrogen (N) deprivation.
CC {ECO:0000269|PubMed:16021502, ECO:0000269|PubMed:17148611,
CC ECO:0000269|PubMed:9430595}.
CC -!- DISRUPTION PHENOTYPE: Impaired lateral root proliferation in nitrate
CC rich root zones. {ECO:0000269|PubMed:16021502,
CC ECO:0000269|PubMed:9430595}.
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DR EMBL; Z97057; CAB09793.1; -; mRNA.
DR EMBL; AC007210; AAD25638.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06290.1; -; Genomic_DNA.
DR EMBL; BT005861; AAO64796.1; -; mRNA.
DR EMBL; AK228244; BAF00192.1; -; mRNA.
DR PIR; A84515; A84515.
DR PIR; T52100; T52100.
DR RefSeq; NP_179033.1; NM_126990.3.
DR AlphaFoldDB; Q9SI38; -.
DR SMR; Q9SI38; -.
DR BioGRID; 1267; 8.
DR IntAct; Q9SI38; 8.
DR STRING; 3702.AT2G14210.1; -.
DR PaxDb; Q9SI38; -.
DR PRIDE; Q9SI38; -.
DR EnsemblPlants; AT2G14210.1; AT2G14210.1; AT2G14210.
DR GeneID; 815907; -.
DR Gramene; AT2G14210.1; AT2G14210.1; AT2G14210.
DR KEGG; ath:AT2G14210; -.
DR Araport; AT2G14210; -.
DR TAIR; locus:2042182; AT2G14210.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_2_0_1; -.
DR InParanoid; Q9SI38; -.
DR OMA; IMIRRID; -.
DR PhylomeDB; Q9SI38; -.
DR PRO; PR:Q9SI38; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SI38; baseline and differential.
DR Genevisible; Q9SI38; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0071249; P:cellular response to nitrate; IEP:UniProtKB.
DR GO; GO:0048527; P:lateral root development; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0010167; P:response to nitrate; IMP:UniProtKB.
DR GO; GO:0007584; P:response to nutrient; IMP:TAIR.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..234
FT /note="MADS-box transcription factor ANR1"
FT /id="PRO_0000403962"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 87..177
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT CONFLICT 103
FT /note="Y -> H (in Ref. 1; CAB09793)"
FT /evidence="ECO:0000305"
FT CONFLICT 191
FT /note="A -> V (in Ref. 1; CAB09793)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 234 AA; 26803 MW; 720F16BC1F72DE27 CRC64;
MGRGKIVIRR IDNSTSRQVT FSKRRSGLLK KAKELSILCD AEVGVIIFSS TGKLYDYASN
SSMKTIIERY NRVKEEQHQL LNHASEIKFW QREVASLQQQ LQYLQECHRK LVGEELSGMN
ANDLQNLEDQ LVTSLKGVRL KKDQLMTNEI RELNRKGQII QKENHELQNI VDIMRKENIK
LQKKVHGRTN AIEGNSSVDP ISNGTTTYAP PQLQLIQLQP APREKSIRLG LQLS
