ANR2_YEAST
ID ANR2_YEAST Reviewed; 516 AA.
AC P36090; D6VXP0;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Uncharacterized protein ANR2;
DE AltName: Full=AVL9-related family protein 2;
GN Name=ANR2; OrderedLocusNames=YKL047W; ORFNames=YKL260;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8154189; DOI=10.1002/yea.320091212;
RA Purnelle B., Tettelin H., van Dyck L., Skala J., Goffeau A.;
RT "The sequence of a 17.5 kb DNA fragment on the left arm of yeast chromosome
RT XI identifies the protein kinase gene ELM1, the DNA primase gene PRI2, a
RT new gene encoding a putative histone and seven new open reading frames.";
RL Yeast 9:1379-1384(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PALMITOYLATION.
RX PubMed=16751107; DOI=10.1016/j.cell.2006.03.042;
RA Roth A.F., Wan J., Bailey A.O., Sun B., Kuchar J.A., Green W.N.,
RA Phinney B.S., Yates J.R. III, Davis N.G.;
RT "Global analysis of protein palmitoylation in yeast.";
RL Cell 125:1003-1013(2006).
RN [7]
RP GENE NAME.
RX PubMed=17229886; DOI=10.1091/mbc.e06-11-1035;
RA Harsay E., Schekman R.;
RT "Avl9p, a member of a novel protein superfamily, functions in the late
RT secretory pathway.";
RL Mol. Biol. Cell 18:1203-1219(2007).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=24868093; DOI=10.1194/jlr.m050229;
RA Currie E., Guo X., Christiano R., Chitraju C., Kory N., Harrison K.,
RA Haas J., Walther T.C., Farese R.V. Jr.;
RT "High-confidence proteomic analysis of yeast lipid droplets identifies
RT additional droplet proteins and reveals connections to dolichol synthesis
RT and sterol acetylation.";
RL J. Lipid Res. 55:1465-1477(2014).
CC -!- FUNCTION: May be involved in lipid metabolism.
CC {ECO:0000269|PubMed:24868093}.
CC -!- SUBCELLULAR LOCATION: Lipid droplet {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:24868093}.
CC -!- PTM: Palmitoylated by AKR1. {ECO:0000269|PubMed:16751107}.
CC -!- MISCELLANEOUS: Present with 2550 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X71621; CAA50627.1; -; Genomic_DNA.
DR EMBL; Z28047; CAA81882.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09110.1; -; Genomic_DNA.
DR PIR; S37868; S37868.
DR RefSeq; NP_012877.1; NM_001179613.1.
DR AlphaFoldDB; P36090; -.
DR BioGRID; 34086; 45.
DR DIP; DIP-3998N; -.
DR IntAct; P36090; 6.
DR MINT; P36090; -.
DR STRING; 4932.YKL047W; -.
DR iPTMnet; P36090; -.
DR SwissPalm; P36090; -.
DR MaxQB; P36090; -.
DR PaxDb; P36090; -.
DR PRIDE; P36090; -.
DR EnsemblFungi; YKL047W_mRNA; YKL047W; YKL047W.
DR GeneID; 853819; -.
DR KEGG; sce:YKL047W; -.
DR SGD; S000001530; ANR2.
DR VEuPathDB; FungiDB:YKL047W; -.
DR eggNOG; KOG4704; Eukaryota.
DR HOGENOM; CLU_019791_0_0_1; -.
DR InParanoid; P36090; -.
DR OMA; KSWRHAP; -.
DR BioCyc; YEAST:G3O-31848-MON; -.
DR PRO; PR:P36090; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36090; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005811; C:lipid droplet; IDA:SGD.
DR InterPro; IPR028115; DUF4484.
DR InterPro; IPR018626; LCHN/Anr2.
DR InterPro; IPR037516; Tripartite_DENN.
DR Pfam; PF09804; DENND11; 1.
DR Pfam; PF14831; DUF4484; 1.
DR PROSITE; PS50211; DENN; 1.
PE 1: Evidence at protein level;
KW Lipid droplet; Lipoprotein; Palmitate; Reference proteome.
FT CHAIN 1..516
FT /note="Uncharacterized protein ANR2"
FT /id="PRO_0000203181"
FT DOMAIN 31..185
FT /note="uDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 211..365
FT /note="cDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 367..513
FT /note="dDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT LIPID 511
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 516
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 516 AA; 58895 MW; 18923F69D85F59D8 CRC64;
MNSGGEEPTI KPNVFNITQL LNSNGEKPGI ACIFLSKFDM KKGNIIIWSK SINGAAIDLS
NIEFKSLPAG IHEQTDDVVN FVVPKELDVC QTAKTTTYDY GIAYFKQNSF DIIENDNRID
RSKVQMFSLG VIIDVQNASS DSKKHFYKEI YHAYAANRYS SYLESLLGQW IRQRDLDKFD
IFEKFFDENN QGHMAENSVE VFEHSPKERR HLVEYLPYWT RKLGPLIFPL WKASLLQSRI
LILVPQGESF ELCNSLAYCV FLISMLPKNL IGNHVSDEYI KPIFTVSTSD IPFLESFKKG
NGYVATTSEE ILLYKPEIYD IVVKLTSSST IEESPEKEVE ILTASGEQNK ATPLDLEVYE
KLILGELQED ASTNATCRHH EVTEPISWLQ FLIDGFFLLT TAGYLVAPYH LANNFKIPRH
VSGPEPNNSE IQIAENLVRY FHRRTSNLYN DLKDVIQKSE NIDSEQPITI AASFLTKLNL
DCFSKQDHQF VKDIALKWFQ RSIDISNLPE CLGNLC
