ID   3SOA1_DENAN             Reviewed;          80 AA.
AC   P18329;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Toxin C13S1C1;
DE   Flags: Precursor;
OS   Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Dendroaspis.
OX   NCBI_TaxID=8618;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RA   Ducancel F.;
RL   Submitted (MAR-1990) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 22-80, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=7397227; DOI=10.1016/0005-2795(80)90274-3;
RA   Joubert F.J., Taljaard N.;
RT   "The complete primary structures of two reduced and S-carboxymethylated
RT   Angusticeps-type toxins from Dendroaspis angusticeps (green mamba) venom.";
RL   Biochim. Biophys. Acta 623:449-456(1980).
RN   [3]
RP   FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=25035794;
RA   Conlon J.M., Prajeep M., Mechkarska M., Arafat K., Attoub S., Adem A.,
RA   Pla D., Calvete J.J.;
RT   "Peptides with in vitro anti-tumor activity from the venom of the Eastern
RT   green mamba, Dendroaspis angusticeps (Elapidae).";
RL   J. Venom Res. 5:16-21(2014).
CC   -!- FUNCTION: Is cytotoxic against human non-small cell lung adenocarcinoma
CC       A-549 cells (LC(50)=56 ug/ml), breast adenocarcinoma MDA-MBsy-231 cells
CC       (LC(50)=62 ug/ml) and colorectal adenocarcinoma HT-29 cells (LC(50)=110
CC       ug/ml). Shows low hemolytic activity against human erythrocytes
CC       (LC(50)>600 ug/ml). Is also cytotoxic against human umbilical vein
CC       endothelial HUVEC cells (57 ug/ml) indicating no differential activity
CC       against cell lines derived from neoplastic tissues.
CC       {ECO:0000269|PubMed:25035794}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:7397227}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=6653.0; Method=Unknown;
CC       Evidence={ECO:0000269|PubMed:25035794};
CC   -!- TOXIC DOSE: LD(50) is 40 +/- 3 mg/kg by intravenous injection into
CC       mice. {ECO:0000269|PubMed:7397227}.
CC   -!- MISCELLANEOUS: Does not inhibit growth of reference strains of E.coli
CC       or S.aureus (MIC values >200 ug/ml). {ECO:0000269|PubMed:25035794}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Orphan group X sub-subfamily. {ECO:0000305}.
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DR   EMBL; X52260; CAA36503.1; -; mRNA.
DR   EMBL; X53410; CAA37486.1; -; mRNA.
DR   PIR; S10464; S10464.
DR   AlphaFoldDB; P18329; -.
DR   SMR; P18329; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:7397227"
FT   CHAIN           22..80
FT                   /note="Toxin C13S1C1"
FT                   /evidence="ECO:0000269|PubMed:7397227"
FT                   /id="PRO_0000035484"
FT   DISULFID        24..43
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        38..60
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        62..73
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        74..79
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   CONFLICT        56
FT                   /note="Missing (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   80 AA;  8954 MW;  5F4450DF19E721C8 CRC64;
     MKTLLLTLLV VTIVCLDLAH TRICYSHKLL QAKTTKTCEE NSCYKRSLPK IPLIIIGRGC
     GCPLTLPFLR IKCCTSDKCN