ANR44_HUMAN
ID ANR44_HUMAN Reviewed; 993 AA.
AC Q8N8A2; Q53SL9; Q6P480; Q86VL5; Q8IZ72; Q9UFA4;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 3.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B;
DE Short=PP6-ARS-B;
DE Short=Serine/threonine-protein phosphatase 6 regulatory subunit ARS-B;
DE AltName: Full=Ankyrin repeat domain-containing protein 44;
GN Name=ANKRD44;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC TISSUE=Spleen;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 5), AND NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] OF 812-989 (ISOFORM 1).
RC TISSUE=Duodenum, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 204-993 (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [5]
RP INTERACTION WITH PPP6R1.
RX PubMed=18186651; DOI=10.1021/bi7022877;
RA Stefansson B., Ohama T., Daugherty A.E., Brautigan D.L.;
RT "Protein phosphatase 6 regulatory subunits composed of ankyrin repeat
RT domains.";
RL Biochemistry 47:1442-1451(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Putative regulatory subunit of protein phosphatase 6 (PP6)
CC that may be involved in the recognition of phosphoprotein substrates.
CC -!- SUBUNIT: Protein phosphatase 6 (PP6) holoenzyme is proposed to be a
CC heterotrimeric complex formed by the catalytic subunit, a SAPS domain-
CC containing subunit (PP6R) and an ankyrin repeat-domain containing
CC regulatory subunit (ARS). Interacts with PPP6R1.
CC {ECO:0000269|PubMed:18186651}.
CC -!- INTERACTION:
CC Q8N8A2; Q9UPN7: PPP6R1; NbExp=4; IntAct=EBI-1245329, EBI-359745;
CC Q8N8A2; P35125-3: USP6; NbExp=4; IntAct=EBI-1245329, EBI-954590;
CC Q8N8A2; O00308: WWP2; NbExp=5; IntAct=EBI-1245329, EBI-743923;
CC Q8N8A2-2; P04792: HSPB1; NbExp=3; IntAct=EBI-21636328, EBI-352682;
CC Q8N8A2-2; Q9Y3C5: RNF11; NbExp=3; IntAct=EBI-21636328, EBI-396669;
CC Q8N8A2-2; O76024: WFS1; NbExp=3; IntAct=EBI-21636328, EBI-720609;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=Q8N8A2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N8A2-2; Sequence=VSP_019601, VSP_019604;
CC Name=3;
CC IsoId=Q8N8A2-3; Sequence=VSP_019602, VSP_019603;
CC Name=4;
CC IsoId=Q8N8A2-4; Sequence=VSP_019600, VSP_035965;
CC Name=5;
CC IsoId=Q8N8A2-5; Sequence=VSP_035966;
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DR EMBL; AK097086; BAC04946.1; -; mRNA.
DR EMBL; AC010746; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC013264; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC017035; AAY15060.1; -; Genomic_DNA.
DR EMBL; AW629326; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC016985; AAH16985.2; -; mRNA.
DR EMBL; BC050586; AAH50586.2; -; mRNA.
DR EMBL; BC063622; AAH63622.1; -; mRNA.
DR EMBL; AL133087; CAB61404.1; -; mRNA.
DR CCDS; CCDS33355.2; -. [Q8N8A2-5]
DR CCDS; CCDS74619.1; -. [Q8N8A2-1]
DR PIR; T42691; T42691.
DR RefSeq; NP_001182073.1; NM_001195144.1. [Q8N8A2-1]
DR RefSeq; NP_710181.2; NM_153697.2. [Q8N8A2-5]
DR RefSeq; XP_005247005.1; XM_005246948.2. [Q8N8A2-2]
DR AlphaFoldDB; Q8N8A2; -.
DR SMR; Q8N8A2; -.
DR BioGRID; 124841; 48.
DR IntAct; Q8N8A2; 28.
DR MINT; Q8N8A2; -.
DR STRING; 9606.ENSP00000282272; -.
DR ChEMBL; CHEMBL4105772; -.
DR GlyGen; Q8N8A2; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q8N8A2; -.
DR PhosphoSitePlus; Q8N8A2; -.
DR BioMuta; ANKRD44; -.
DR DMDM; 218512105; -.
DR EPD; Q8N8A2; -.
DR jPOST; Q8N8A2; -.
DR MassIVE; Q8N8A2; -.
DR MaxQB; Q8N8A2; -.
DR PaxDb; Q8N8A2; -.
DR PeptideAtlas; Q8N8A2; -.
DR PRIDE; Q8N8A2; -.
DR ProteomicsDB; 72386; -. [Q8N8A2-1]
DR ProteomicsDB; 72387; -. [Q8N8A2-2]
DR ProteomicsDB; 72388; -. [Q8N8A2-3]
DR ProteomicsDB; 72389; -. [Q8N8A2-4]
DR ProteomicsDB; 72390; -. [Q8N8A2-5]
DR Antibodypedia; 34063; 93 antibodies from 18 providers.
DR DNASU; 91526; -.
DR Ensembl; ENST00000282272.15; ENSP00000282272.9; ENSG00000065413.20. [Q8N8A2-1]
DR Ensembl; ENST00000328737.6; ENSP00000331516.2; ENSG00000065413.20. [Q8N8A2-4]
DR Ensembl; ENST00000409153.5; ENSP00000387141.1; ENSG00000065413.20. [Q8N8A2-3]
DR Ensembl; ENST00000409919.5; ENSP00000387233.1; ENSG00000065413.20. [Q8N8A2-5]
DR GeneID; 91526; -.
DR KEGG; hsa:91526; -.
DR MANE-Select; ENST00000282272.15; ENSP00000282272.9; NM_001195144.2; NP_001182073.1.
DR UCSC; uc002uub.5; human. [Q8N8A2-1]
DR CTD; 91526; -.
DR DisGeNET; 91526; -.
DR GeneCards; ANKRD44; -.
DR HGNC; HGNC:25259; ANKRD44.
DR HPA; ENSG00000065413; Tissue enhanced (lymphoid).
DR neXtProt; NX_Q8N8A2; -.
DR OpenTargets; ENSG00000065413; -.
DR PharmGKB; PA142672611; -.
DR VEuPathDB; HostDB:ENSG00000065413; -.
DR eggNOG; KOG0504; Eukaryota.
DR eggNOG; KOG4177; Eukaryota.
DR GeneTree; ENSGT00950000182908; -.
DR HOGENOM; CLU_000134_58_0_1; -.
DR InParanoid; Q8N8A2; -.
DR OMA; KCFRKFV; -.
DR OrthoDB; 1115202at2759; -.
DR PhylomeDB; Q8N8A2; -.
DR TreeFam; TF329520; -.
DR PathwayCommons; Q8N8A2; -.
DR SignaLink; Q8N8A2; -.
DR BioGRID-ORCS; 91526; 10 hits in 1076 CRISPR screens.
DR ChiTaRS; ANKRD44; human.
DR GenomeRNAi; 91526; -.
DR Pharos; Q8N8A2; Tdark.
DR PRO; PR:Q8N8A2; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q8N8A2; protein.
DR Bgee; ENSG00000065413; Expressed in monocyte and 177 other tissues.
DR ExpressionAtlas; Q8N8A2; baseline and differential.
DR Genevisible; Q8N8A2; HS.
DR Gene3D; 1.25.40.20; -; 10.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR Pfam; PF12796; Ank_2; 11.
DR Pfam; PF13637; Ank_4; 1.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 28.
DR SUPFAM; SSF48403; SSF48403; 4.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 23.
PE 1: Evidence at protein level;
KW Alternative splicing; ANK repeat; Reference proteome; Repeat.
FT CHAIN 1..993
FT /note="Serine/threonine-protein phosphatase 6 regulatory
FT ankyrin repeat subunit B"
FT /id="PRO_0000244572"
FT REPEAT 7..36
FT /note="ANK 1"
FT REPEAT 40..69
FT /note="ANK 2"
FT REPEAT 73..102
FT /note="ANK 3"
FT REPEAT 106..135
FT /note="ANK 4"
FT REPEAT 139..168
FT /note="ANK 5"
FT REPEAT 172..201
FT /note="ANK 6"
FT REPEAT 205..234
FT /note="ANK 7"
FT REPEAT 238..267
FT /note="ANK 8"
FT REPEAT 271..301
FT /note="ANK 9"
FT REPEAT 305..334
FT /note="ANK 10"
FT REPEAT 338..367
FT /note="ANK 11"
FT REPEAT 371..400
FT /note="ANK 12"
FT REPEAT 404..433
FT /note="ANK 13"
FT REPEAT 437..466
FT /note="ANK 14"
FT REPEAT 470..498
FT /note="ANK 15"
FT REPEAT 531..561
FT /note="ANK 16"
FT REPEAT 566..595
FT /note="ANK 17"
FT REPEAT 599..628
FT /note="ANK 18"
FT REPEAT 633..662
FT /note="ANK 19"
FT REPEAT 669..698
FT /note="ANK 20"
FT REPEAT 702..731
FT /note="ANK 21"
FT REPEAT 735..764
FT /note="ANK 22"
FT REPEAT 771..800
FT /note="ANK 23"
FT REPEAT 803..832
FT /note="ANK 24"
FT REPEAT 838..867
FT /note="ANK 25"
FT REPEAT 871..901
FT /note="ANK 26"
FT REPEAT 905..934
FT /note="ANK 27"
FT REPEAT 941..970
FT /note="ANK 28"
FT VAR_SEQ 1..25
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_019600"
FT VAR_SEQ 368..993
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_035966"
FT VAR_SEQ 395
FT /note="G -> GQKYSIVSLFSNEHVLSAG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_019601"
FT VAR_SEQ 551..579
FT /note="LLERTNSGFEESDSGATKSPLHLAAYNGH -> VSCFVLFSLTHTHTHTPNH
FT RGINCFFPEL (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_019602"
FT VAR_SEQ 580..993
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_019603"
FT VAR_SEQ 945..993
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_035965"
FT VAR_SEQ 975..993
FT /note="ASRSNGPRSTPGTAVQKEE -> GC (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_019604"
FT CONFLICT 205
FT /note="K -> R (in Ref. 4; CAB61404)"
FT /evidence="ECO:0000305"
FT CONFLICT 359
FT /note="I -> R (in Ref. 1; BAC04946)"
FT /evidence="ECO:0000305"
FT CONFLICT 622
FT /note="Q -> P (in Ref. 1; BAC04946)"
FT /evidence="ECO:0000305"
FT CONFLICT 844
FT /note="H -> R (in Ref. 1; BAC04946)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 993 AA; 107603 MW; 3502EBBD0D093566 CRC64;
MAVLKLTDQP PLVQAIFSGD PEEIRMLIHK TEDVNTLDSE KRTPLHVAAF LGDAEIIELL
ILSGARVNAK DNMWLTPLHR AVASRSEEAV QVLIKHSADV NARDKNWQTP LHVAAANKAV
KCAEVIIPLL SSVNVSDRGG RTALHHAALN GHVEMVNLLL AKGANINAFD KKDRRALHWA
AYMGHLDVVA LLINHGAEVT CKDKKGYTPL HAAASNGQIN VVKHLLNLGV EIDEINVYGN
TALHIACYNG QDAVVNELID YGANVNQPNN NGFTPLHFAA ASTHGALCLE LLVNNGADVN
IQSKDGKSPL HMTAVHGRFT RSQTLIQNGG EIDCVDKDGN TPLHVAARYG HELLINTLIT
SGADTAKCGI HSMFPLHLAA LNAHSDCCRK LLSSGFEIDT PDKFGRTCLH AAAAGGNVEC
IKLLQSSGAD FHKKDKCGRT PLHYAAANCH FHCIETLVTT GANVNETDDW GRTALHYAAA
SDMDRNKTIL GNAHDNSEEL ERARELKEKE ATLCLEFLLQ NDANPSIRDK EGYNSIHYAA
AYGHRQCLEL LLERTNSGFE ESDSGATKSP LHLAAYNGHH QALEVLLQSL VDLDIRDEKG
RTALDLAAFK GHTECVEALI NQGASIFVKD NVTKRTPLHA SVINGHTLCL RLLLEIADNP
EAVDVKDAKG QTPLMLAVAY GHIDAVSLLL EKEANVDTVD ILGCTALHRG IMTGHEECVQ
MLLEQEVSIL CKDSRGRTPL HYAAARGHAT WLSELLQMAL SEEDCCFKDN QGYTPLHWAC
YNGNENCIEV LLEQKCFRKF IGNPFTPLHC AIINDHGNCA SLLLGAIDSS IVSCRDDKGR
TPLHAAAFAD HVECLQLLLR HSAPVNAVDN SGKTALMMAA ENGQAGAVDI LVNSAQADLT
VKDKDLNTPL HLACSKGHEK CALLILDKIQ DESLINEKNN ALQTPLHVAA RNGLKVVVEE
LLAKGACVLA VDENASRSNG PRSTPGTAVQ KEE
