ID   ANR45_DANRE             Reviewed;         224 AA.
AC   B0S794; Q501Y8;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Ankyrin repeat domain-containing protein 45;
GN   Name=ankrd45 {ECO:0000312|ZFIN:ZDB-GENE-050522-311};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Olfactory epithelium;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=31208154; DOI=10.3390/genes10060462;
RA   Kang Y., Xie H., Zhao C.;
RT   "Ankrd45 Is a Novel Ankyrin Repeat Protein Required for Cell
RT   Proliferation.";
RL   Genes (Basel) 10:0-0(2019).
CC   -!- FUNCTION: May play a role during cell division.
CC       {ECO:0000269|PubMed:31208154}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q5TZF3}.
CC       Midbody, Midbody ring {ECO:0000250|UniProtKB:Q5TZF3}. Cleavage furrow
CC       {ECO:0000250|UniProtKB:Q5TZF3}. Note=Distribution is highly dynamic
CC       during mitosis. Not detected during interphase, localized to cytoplasm
CC       during metaphase, to cleavage furrow during anaphase and telophase, and
CC       to midbody ring during cytokinesis. {ECO:0000250|UniProtKB:Q5TZF3}.
CC   -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|Ref.2}.
CC   -!- DEVELOPMENTAL STAGE: Weakly detected in the dorsal forerunner cells at
CC       80% epiboly to bud stages. At the 10-somite stage, strong expression is
CC       detected in the otic placodes, Kupffer's vesicles, and floor plate.
CC       Later, at 24 h post-fertilization, is mainly expressed in organs rich
CC       in motile cilia, including otic vesicles, pronephric ducts, and floor
CC       plates. At later stages, displays a ubiquitous expression pattern.
CC       {ECO:0000269|Ref.2}.
CC   -!- DISRUPTION PHENOTYPE: Ankrd45 mutants are viable and show no apparent
CC       defects. However mutant larvae developed enlarged livers when induced
CC       with liver specific expression of Kras G12V, one of the common
CC       mutations of KRAS that leads to cancer in humans.
CC       {ECO:0000269|PubMed:31208154}.
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DR   EMBL; FP015808; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; FP016213; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC095811; AAH95811.1; -; mRNA.
DR   RefSeq; NP_001018606.1; NM_001020770.1.
DR   AlphaFoldDB; B0S794; -.
DR   SMR; B0S794; -.
DR   STRING; 7955.ENSDARP00000020123; -.
DR   PaxDb; B0S794; -.
DR   PeptideAtlas; B0S794; -.
DR   Ensembl; ENSDART00000002094; ENSDARP00000020123; ENSDARG00000011326.
DR   GeneID; 553808; -.
DR   KEGG; dre:553808; -.
DR   CTD; 339416; -.
DR   ZFIN; ZDB-GENE-050522-311; ankrd45.
DR   eggNOG; KOG0100; Eukaryota.
DR   GeneTree; ENSGT00390000008829; -.
DR   HOGENOM; CLU_087014_0_0_1; -.
DR   InParanoid; B0S794; -.
DR   OMA; CRVKSDW; -.
DR   OrthoDB; 1354745at2759; -.
DR   PhylomeDB; B0S794; -.
DR   TreeFam; TF329503; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 2.
DR   Bgee; ENSDARG00000011326; Expressed in Kupffer's vesicle and 27 other tissues.
DR   ExpressionAtlas; B0S794; baseline.
DR   GO; GO:0032154; C:cleavage furrow; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0090543; C:Flemming body; IEA:UniProtKB-SubCell.
DR   GO; GO:0030496; C:midbody; ISS:UniProtKB.
DR   GO; GO:0008283; P:cell population proliferation; IMP:UniProtKB.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 1.25.40.20; -; 1.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   Pfam; PF12796; Ank_2; 1.
DR   SMART; SM00248; ANK; 2.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF48403; SSF48403; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 1.
DR   PROSITE; PS50088; ANK_REPEAT; 1.
PE   2: Evidence at transcript level;
KW   ANK repeat; Cytoplasm; Reference proteome; Repeat.
FT   CHAIN           1..224
FT                   /note="Ankyrin repeat domain-containing protein 45"
FT                   /id="PRO_0000453973"
FT   REPEAT          46..76
FT                   /note="ANK 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          80..109
FT                   /note="ANK 2"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        180
FT                   /note="D -> E (in Ref. 2; AAH95811)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        192
FT                   /note="D -> V (in Ref. 2; AAH95811)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   224 AA;  25070 MW;  20438C583DB7609F CRC64;
     MRSAEEKTVL LCALDDDLEG LKGILERTFT DDAAQSENIL WEKDEVGRNA LFAACMMGRS
     AIVRELVQNG AADVNELTAR GYSPLHCSAM WGQLDTLKTL VELNADFQAI NFRGEKAVDV
     ARRYDKLDCA EYLAWAEAKQ NLQAFIQEVR AIVADQEKVQ GKLNKEDKNI CINTCSAKSD
     WINNTRTATA QDFIEQKKLL EDVLAPVLLK LNAQPEASMK TRKN