ANR53_MOUSE
ID ANR53_MOUSE Reviewed; 497 AA.
AC Q3V0J4; E9QN79;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Ankyrin repeat domain-containing protein 53;
GN Name=Ankrd53;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Required for normal progression through mitosis. Involved in
CC chromosome alignment and cytokinesis via regulation of microtubules
CC polymerization. {ECO:0000250|UniProtKB:Q8N9V6}.
CC -!- SUBUNIT: Interacts with PSRC1; recruited by PSRC1 to the spindle during
CC mitosis. {ECO:0000250|UniProtKB:Q8N9V6}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q8N9V6}. Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000250|UniProtKB:Q8N9V6}. Note=Localizes at the spindle around
CC the centrosome at prophase and prometaphase and at the spindle poles at
CC metaphase and anaphase. {ECO:0000250|UniProtKB:Q8N9V6}.
CC -!- PTM: Phosphorylated during mitosis. {ECO:0000250|UniProtKB:Q8N9V6}.
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DR EMBL; AK133099; BAE21510.1; -; mRNA.
DR EMBL; AC090647; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS20284.1; -.
DR RefSeq; NP_083521.2; NM_029245.3.
DR AlphaFoldDB; Q3V0J4; -.
DR SMR; Q3V0J4; -.
DR STRING; 10090.ENSMUSP00000014891; -.
DR iPTMnet; Q3V0J4; -.
DR PhosphoSitePlus; Q3V0J4; -.
DR PaxDb; Q3V0J4; -.
DR PRIDE; Q3V0J4; -.
DR ProteomicsDB; 282003; -.
DR Antibodypedia; 53588; 176 antibodies from 17 providers.
DR Ensembl; ENSMUST00000014891; ENSMUSP00000014891; ENSMUSG00000014747.
DR GeneID; 75305; -.
DR KEGG; mmu:75305; -.
DR UCSC; uc009cod.1; mouse.
DR CTD; 79998; -.
DR MGI; MGI:1922555; Ankrd53.
DR VEuPathDB; HostDB:ENSMUSG00000014747; -.
DR eggNOG; KOG4177; Eukaryota.
DR GeneTree; ENSGT00390000005650; -.
DR HOGENOM; CLU_038440_0_0_1; -.
DR InParanoid; Q3V0J4; -.
DR OMA; PCIHYLL; -.
DR OrthoDB; 1055342at2759; -.
DR PhylomeDB; Q3V0J4; -.
DR TreeFam; TF351267; -.
DR BioGRID-ORCS; 75305; 3 hits in 71 CRISPR screens.
DR PRO; PR:Q3V0J4; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q3V0J4; protein.
DR Bgee; ENSMUSG00000014747; Expressed in testis and 29 other tissues.
DR ExpressionAtlas; Q3V0J4; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005819; C:spindle; ISS:UniProtKB.
DR GO; GO:0000922; C:spindle pole; ISS:UniProtKB.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007080; P:mitotic metaphase plate congression; ISS:UniProtKB.
DR GO; GO:0031116; P:positive regulation of microtubule polymerization; ISS:UniProtKB.
DR GO; GO:1902412; P:regulation of mitotic cytokinesis; ISS:UniProtKB.
DR GO; GO:0060236; P:regulation of mitotic spindle organization; ISS:UniProtKB.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR042335; ANKRD53.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR PANTHER; PTHR24160; PTHR24160; 1.
DR Pfam; PF12796; Ank_2; 1.
DR SMART; SM00248; ANK; 3.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 2.
PE 2: Evidence at transcript level;
KW ANK repeat; Cell cycle; Cell division; Coiled coil; Cytoplasm;
KW Cytoskeleton; Mitosis; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..497
FT /note="Ankyrin repeat domain-containing protein 53"
FT /id="PRO_0000320070"
FT REPEAT 110..140
FT /note="ANK 1"
FT REPEAT 144..177
FT /note="ANK 2"
FT REPEAT 181..210
FT /note="ANK 3"
FT REGION 1..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 239..264
FT /evidence="ECO:0000255"
FT COMPBIAS 23..37
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..65
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 220
FT /note="D -> N (in Ref. 1; BAE21510)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 497 AA; 56738 MW; 488312022C5760D4 CRC64;
MRRPSRRRSK ASTPPRSHTT PRRTGPSDSR RRPGTKEQPR PSVQGGTRQA EHDLKVSSPN
SESSQYSTSE LWSHKVIANY SELFAASVGN VDWLRFCVNP ERKEIIVDDK GFTAIHFAAQ
KCQLSCLKVL IEEYKYPVDL PTNKGQTPLH LVIHKNNKSD ILPCIDYLLK KGAAINSQTY
NGSTPLHLAS CNGLLGCIKL LVQSGANVHA RDATGFKPID YCRLWNHRTC ARFLKDVMWK
HDKKVLAQEM EKLRTLKEKL TILEYHYLVE YQKEHQILRE AHFRKWLQNK VLAQTLGSAD
SKQKAGVQPW SLASNTLRCP ITESLHYPSV EAQLKNLPSP VVPPKPIYKQ TTISRPKLWN
YSANPARSPI TNIGHPQNIR LGVHPEPYKE HDFRRFLEVT RNKHGGACLR TVDRQLVTPV
PQLPFEMMVR VLYPGTQPYR MKVPQGLYPR DILKVPEKRH VGDTCSNTMA MTLRETFDKP
FLDSLEVCRT RVAPPSK
