ID   3SOB8_DENAN             Reviewed;          81 AA.
AC   P01404;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 2.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Toxin F-VIII;
DE   AltName: Full=Toxin DaF8;
DE   AltName: Full=Toxin TA2 {ECO:0000303|PubMed:4203470};
DE   Flags: Precursor;
OS   Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Dendroaspis.
OX   NCBI_TaxID=8618;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RA   Ducancel F.;
RL   Submitted (JUN-1990) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 22-81, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=4203470; DOI=10.1016/s0021-9258(19)43039-1;
RA   Viljoen C.C., Botes D.P.;
RT   "Snake venom toxins. The purification and amino acid sequence of toxin TA2
RT   from Dendroaspis angusticeps venom.";
RL   J. Biol. Chem. 249:366-372(1974).
RN   [3]
RP   FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=25035794;
RA   Conlon J.M., Prajeep M., Mechkarska M., Arafat K., Attoub S., Adem A.,
RA   Pla D., Calvete J.J.;
RT   "Peptides with in vitro anti-tumor activity from the venom of the Eastern
RT   green mamba, Dendroaspis angusticeps (Elapidae).";
RL   J. Venom Res. 5:16-21(2014).
CC   -!- FUNCTION: Is cytotoxic against A549 cells (LC(50)=106 ug/ml).
CC       {ECO:0000269|PubMed:25035794}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:4203470}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=6597.8; Method=Unknown; Note=Average mass.;
CC       Evidence={ECO:0000269|PubMed:25035794};
CC   -!- TOXIC DOSE: LD(50) is >250 mg/kg by subcutaneous injection.
CC       {ECO:0000269|PubMed:4203470}.
CC   -!- MISCELLANEOUS: Is not cytotoxic to MDA-MB-231, HT-29 cells, and HUVEC
CC       cells at concentrations up to 300 ug/ml and is not hemolytic at
CC       concentrations up to 1mg/ml. Does not inhibit the growth of reference
CC       strains of E. coli or S. aureus (MIC values >200 ug/ml).
CC       {ECO:0000269|PubMed:25035794}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Orphan group XI sub-subfamily. {ECO:0000305}.
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DR   EMBL; X53409; CAA37485.1; -; mRNA.
DR   PIR; S21299; T5EP2A.
DR   AlphaFoldDB; P01404; -.
DR   SMR; P01404; -.
DR   PRIDE; P01404; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:4203470"
FT   CHAIN           22..81
FT                   /note="Toxin F-VIII"
FT                   /evidence="ECO:0000269|PubMed:4203470"
FT                   /id="PRO_0000035483"
FT   DISULFID        24..43
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        38..60
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        62..73
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   DISULFID        74..79
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT   CONFLICT        54
FT                   /note="I -> V (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   81 AA;  8849 MW;  733195E17A656C97 CRC64;
     MKTLLLTLLV VTIVCLDLAS TMICYSHKTP QPSATITCEE KTCYKKSVRK LPAIVAGRGC
     GCPSKEMLVA IHCCRSDKCN E