ANRA2_MOUSE
ID ANRA2_MOUSE Reviewed; 312 AA.
AC Q99PE2;
DT 10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Ankyrin repeat family A protein 2;
DE AltName: Full=RFXANK-like protein 2;
GN Name=Ankra2; Synonyms=Ankra;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH LRP2, AND
RP SUBCELLULAR LOCATION.
RX PubMed=11095640; DOI=10.1681/asn.v11122167;
RA Rader K., Orlando R.A., Lou X., Farquhar M.G.;
RT "Characterization of ANKRA, a novel ankyrin repeat protein that interacts
RT with the cytoplasmic domain of megalin.";
RL J. Am. Soc. Nephrol. 11:2167-2178(2000).
RN [2]
RP INTERACTION WITH AHRR.
RX PubMed=17949687; DOI=10.1016/j.bbrc.2007.09.131;
RA Oshima M., Mimura J., Yamamoto M., Fujii-Kuriyama Y.;
RT "Molecular mechanism of transcriptional repression of AhR repressor
RT involving ANKRA2, HDAC4, and HDAC5.";
RL Biochem. Biophys. Res. Commun. 364:276-282(2007).
CC -!- FUNCTION: May regulate the interaction between the 3M complex and the
CC histone deacetylases HDAC4 and HDAC5 (By similarity). May also regulate
CC LRP2/megalin (PubMed:11095640). {ECO:0000250|UniProtKB:Q9H9E1,
CC ECO:0000269|PubMed:11095640}.
CC -!- SUBUNIT: Interacts (via ANK repeats) with CCDC8 (via PxLPxI/L motif);
CC mediates the interaction with the 3M complex which is composed of
CC CCDC8, CUL7 and OBSL1. Interacts (via ANK repeats) with HDAC4 (via
CC PxLPxI/L motif). Interacts (via ANK repeats) with HDAC5 (via PxLPxI/L
CC motif) (By similarity). Interacts (via ANK repeats) with LRP2/megalin
CC (via PxLPxI/L motif) (PubMed:11095640). Interacts (via ANK repeats)
CC with RFX7 (via PxLPxI/L motif) (By similarity). Interacts with AHRR
CC (PubMed:17949687). Interacts with NEK6 (By similarity).
CC {ECO:0000250|UniProtKB:Q9H9E1, ECO:0000269|PubMed:11095640,
CC ECO:0000269|PubMed:17949687}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:11095640}. Membrane {ECO:0000269|PubMed:11095640};
CC Peripheral membrane protein {ECO:0000269|PubMed:11095640}.
CC -!- DOMAIN: The ankyrin repeats, mainly ANK 2, ANK 3 and ANK 4, mediate
CC interaction with a wide array of PxLPxI/L motif-containing proteins
CC including HDAC4 and LRP2. The PxLPxI/L motif of interactors can contain
CC a Ser or a Thr residue in position 2, which phosphorylation prevents
CC the interaction with ANKRA2. {ECO:0000250|UniProtKB:Q9H9E1}.
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DR EMBL; AF314031; AAK01620.1; -; mRNA.
DR CCDS; CCDS26713.1; -.
DR RefSeq; NP_075961.1; NM_023472.2.
DR AlphaFoldDB; Q99PE2; -.
DR SMR; Q99PE2; -.
DR BioGRID; 212926; 2.
DR ELM; Q99PE2; -.
DR IntAct; Q99PE2; 1.
DR STRING; 10090.ENSMUSP00000022164; -.
DR iPTMnet; Q99PE2; -.
DR PhosphoSitePlus; Q99PE2; -.
DR PaxDb; Q99PE2; -.
DR PRIDE; Q99PE2; -.
DR ProteomicsDB; 281884; -.
DR Antibodypedia; 24269; 189 antibodies from 23 providers.
DR DNASU; 68558; -.
DR Ensembl; ENSMUST00000022164; ENSMUSP00000022164; ENSMUSG00000021661.
DR GeneID; 68558; -.
DR KEGG; mmu:68558; -.
DR UCSC; uc007ror.2; mouse.
DR CTD; 57763; -.
DR MGI; MGI:1915808; Ankra2.
DR VEuPathDB; HostDB:ENSMUSG00000021661; -.
DR eggNOG; KOG0502; Eukaryota.
DR GeneTree; ENSGT00940000157156; -.
DR HOGENOM; CLU_078123_0_0_1; -.
DR InParanoid; Q99PE2; -.
DR OrthoDB; 1348558at2759; -.
DR PhylomeDB; Q99PE2; -.
DR TreeFam; TF333112; -.
DR BioGRID-ORCS; 68558; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Ankra2; mouse.
DR PRO; PR:Q99PE2; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q99PE2; protein.
DR Bgee; ENSMUSG00000021661; Expressed in cortical plate and 243 other tissues.
DR ExpressionAtlas; Q99PE2; baseline and differential.
DR Genevisible; Q99PE2; MM.
DR GO; GO:1990393; C:3M complex; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IGI:MGI.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IGI:MGI.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0042826; F:histone deacetylase binding; IDA:MGI.
DR GO; GO:0050750; F:low-density lipoprotein particle receptor binding; ISO:MGI.
DR GO; GO:0019901; F:protein kinase binding; ISO:MGI.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; ISS:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR GO; GO:0043254; P:regulation of protein-containing complex assembly; ISS:UniProtKB.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR Pfam; PF12796; Ank_2; 2.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 3.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 3.
PE 1: Evidence at protein level;
KW ANK repeat; Cytoplasm; Cytoskeleton; Membrane; Reference proteome; Repeat.
FT CHAIN 1..312
FT /note="Ankyrin repeat family A protein 2"
FT /id="PRO_0000066896"
FT REPEAT 147..179
FT /note="ANK 1"
FT REPEAT 180..212
FT /note="ANK 2"
FT REPEAT 213..245
FT /note="ANK 3"
FT REPEAT 246..278
FT /note="ANK 4"
FT REPEAT 279..312
FT /note="ANK 5"
SQ SEQUENCE 312 AA; 34063 MW; 3B52B1415B7A5AFA CRC64;
MATSANLDIG AQLIVEECPS SYISGMPDIK LEHQLDPNPD EGAAQGVAMG MKFILPNRFD
MNVCSRFVKS LNEEDSKNIQ DQVNSDLEVA SVLFKAECNI HTSPSPGIQV RHVYTPSTTK
HFSPIKQSTT LTNKHRGNEV STTPLLANSL SAHQLAAQGE MLYLATRIEQ ENVINHTDEE
GFTPLMWAAA HGQIAVVEFL LQNGADPQLL GKGRESALSL ACSKGYTDIV KMLLDCGVDV
NEYDWNGGTP LLYAVHGNHV KCVKMLLENG ADPTIETDSG YNSMDLAVAL GYRGVQQAIE
SHLLKLLQNI RE
