ID   ANSP2_MYCBO             Reviewed;         487 AA.
AC   P0A4W1; A0A1R3XV15; O06297; X2BES8;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=L-asparagine permease 2;
DE   AltName: Full=L-asparagine transport protein 2;
GN   Name=ansP2; OrderedLocusNames=BQ2027_MB0354C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: Dual function in both nitrogen assimilation and in protection
CC       against acid stress during infection. Involved in asparagine uptake.
CC       {ECO:0000250|UniProtKB:P9WQM7}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC       superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family.
CC       {ECO:0000305}.
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DR   EMBL; LT708304; SIT98907.1; -; Genomic_DNA.
DR   RefSeq; NP_854017.1; NC_002945.3.
DR   RefSeq; WP_003898409.1; NC_002945.4.
DR   AlphaFoldDB; P0A4W1; -.
DR   SMR; P0A4W1; -.
DR   PATRIC; fig|233413.5.peg.385; -.
DR   OMA; LMIPGIQ; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   InterPro; IPR004841; AA-permease/SLC12A_dom.
DR   InterPro; IPR004840; Amoino_acid_permease_CS.
DR   Pfam; PF00324; AA_permease; 1.
DR   PROSITE; PS00218; AMINO_ACID_PERMEASE_1; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..487
FT                   /note="L-asparagine permease 2"
FT                   /id="PRO_0000054188"
FT   TRANSMEM        26..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        50..70
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        163..183
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        214..234
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        256..276
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        290..310
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        341..361
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        369..389
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        414..434
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        440..460
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   487 AA;  52195 MW;  3572502DB6ACD987 CRC64;
     MPPLDITDER LTREDTGYHK GLHSRQLQMI ALGGAIGTGL FLGAGGRLAS AGPGLFLVYG
     ICGIFVFLIL RALGELVLHR PSSGSFVSYA REFYGEKVAF VAGWMYFLNW AMTGIVDTTA
     IAHYCHYWRA FQPIPQWTLA LIALLVVLSM NLISVRLFGE LEFWASLIKV IALVTFLIVG
     TVFLAGRYKI DGQETGVSLW SSHGGIVPTG LLPIVLVTSG VVFAYAAIEL VGIAAGETAE
     PAKIMPRAIN SVVLRIACFY VGSTVLLALL LPYTAYKEHV SPFVTFFSKI GIDAAGSVMN
     LVVLTAALSS LNAGLYSTGR ILRSMAINGS GPRFTAPMSK TGVPYGGILL TAGIGLLGII
     LNAIKPSQAF EIVLHIAATG VIAAWATIVA CQLRLHRMAN AGQLQRPKFR MPLSPFSGYL
     TLAFLAGVLI LMYFDEQHGP WMIAATVIGV PALIGGWYLV RNRVTAVAHH AIDHTKSVAV
     VHSADPI