ID   ANTA_HYDVU              Reviewed;         220 AA.
AC   P38977;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Antistasin;
DE            Short=ATS;
DE   AltName: Full=Blood coagulation factor Xa/proclotting enzyme inhibitor;
DE   Flags: Precursor;
OS   Hydra vulgaris (Hydra) (Hydra attenuata).
OC   Eukaryota; Metazoa; Cnidaria; Hydrozoa; Hydroidolina; Anthoathecata;
OC   Aplanulata; Hydridae; Hydra.
OX   NCBI_TaxID=6087;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=SF1;
RX   PubMed=1516699; DOI=10.1016/0014-5793(92)80791-e;
RA   Holstein T.W., Mala C., Kurz E., Bauer K., Greber M., David C.N.;
RT   "The primitive metazoan Hydra expresses antistasin, a serine protease
RT   inhibitor of vertebrate blood coagulation: cDNA cloning, cellular
RT   localisation and developmental regulation.";
RL   FEBS Lett. 309:288-292(1992).
CC   -!- FUNCTION: This highly disulfide-bonded protein is a potent inhibitor of
CC       factor Xa. Facilitates digestion of tissues and may also protect the
CC       gastric tissues from its own digestive enzymes. May have therapeutic
CC       utility as an anticoagulant. Also exhibits a strong metastatic
CC       activity.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Gland cells. It is more strongly expressed in the
CC       head than in the gastric tissue.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I15 (antistasin) family.
CC       {ECO:0000305}.
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DR   EMBL; X67590; CAA47864.1; -; mRNA.
DR   PIR; S29195; S29195.
DR   AlphaFoldDB; P38977; -.
DR   SMR; P38977; -.
DR   MEROPS; I15.003; -.
DR   Proteomes; UP000694840; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008201; F:heparin binding; IEA:UniProtKB-KW.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0007596; P:blood coagulation; IEA:UniProtKB-KW.
DR   InterPro; IPR004094; Antistasin-like.
DR   InterPro; IPR011061; Hirudin/antistatin.
DR   Pfam; PF02822; Antistasin; 6.
DR   SUPFAM; SSF57262; SSF57262; 6.
DR   PROSITE; PS51252; ANTISTASIN; 6.
PE   2: Evidence at transcript level;
KW   Blood coagulation; Hemostasis; Heparin-binding; Protease inhibitor;
KW   Reference proteome; Repeat; Secreted; Serine protease inhibitor; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..220
FT                   /note="Antistasin"
FT                   /id="PRO_0000001699"
FT   DOMAIN          21..46
FT                   /note="Antistasin-like 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00582"
FT   DOMAIN          54..79
FT                   /note="Antistasin-like 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00582"
FT   DOMAIN          91..117
FT                   /note="Antistasin-like 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00582"
FT   DOMAIN          120..145
FT                   /note="Antistasin-like 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00582"
FT   DOMAIN          154..180
FT                   /note="Antistasin-like 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00582"
FT   DOMAIN          183..208
FT                   /note="Antistasin-like 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00582"
FT   SITE            27..28
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   SITE            60..61
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   SITE            98..99
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   SITE            161..162
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   220 AA;  25016 MW;  E7987F01900D0278 CRC64;
     MNYLFVFLAL SAAVTFANAE CNKIQCRMFC KFGFQQDENG CDICKCAERP EKKCSNRYCK
     MLCPEGFQVD ANGCQICRCK RSALEAPEKK CDGLKQCKMH CENGFVRDEN GCPKCECSKC
     KQFQCLIFCP HGNEVDENGC KTCKCKAAPE KKKCDDLKQC RMFCENGFVR DENGCKKCEC
     NKCKNFICQI FCEYGNVVDE NGCKTCKCNS KPLKLSLHCR