ID   ANTIH_BPP2              Reviewed;         141 AA.
AC   P51769;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   23-FEB-2022, entry version 74.
DE   RecName: Full=Putative antiholin;
DE   AltName: Full=Protein lysA;
GN   Name=lysA;
OS   Escherichia phage P2 (Bacteriophage P2).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Peduovirinae; Peduovirus.
OX   NCBI_TaxID=10679;
OH   NCBI_TaxID=543; Enterobacteriaceae.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8051010; DOI=10.1128/jb.176.16.4974-4984.1994;
RA   Ziermann R., Bartlett B., Calendar R., Christie G.E.;
RT   "Functions involved in bacteriophage P2-induced host cell lysis and
RT   identification of a new tail gene.";
RL   J. Bacteriol. 176:4974-4984(1994).
RN   [2]
RP   FUNCTION.
RX   PubMed=23335412; DOI=10.1128/jb.01986-12;
RA   To K.H., Dewey J., Weaver J., Park T., Young R.;
RT   "Functional analysis of a class I holin, P2 Y.";
RL   J. Bacteriol. 195:1346-1355(2013).
CC   -!- FUNCTION: Involved in lysis inhibition. Interacts with and inhibits the
CC       holin thereby delaying the host cell lysis timing.
CC       {ECO:0000269|PubMed:23335412}.
CC   -!- SUBUNIT: Interacts with holin; this interaction this interaction blocks
CC       the holin homomultimerization and delays the host cell lysis.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cell inner membrane {ECO:0000305}; Multi-
CC       pass membrane protein {ECO:0000305}.
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DR   EMBL; AF063097; AAD03277.1; -; Genomic_DNA.
DR   PIR; E55855; E55855.
DR   RefSeq; NP_046766.1; NC_001895.1.
DR   SMR; P51769; -.
DR   GeneID; 1261525; -.
DR   KEGG; vg:1261525; -.
DR   Proteomes; UP000009092; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:1901218; P:negative regulation of holin activity; IDA:CACAO.
PE   3: Inferred from homology;
KW   Cytolysis; Host cell inner membrane; Host cell lysis by virus;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Viral release from host cell.
FT   CHAIN           1..141
FT                   /note="Putative antiholin"
FT                   /id="PRO_0000165242"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        94..114
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   141 AA;  15497 MW;  46E0CC198B0B84B2 CRC64;
     MKKLSLSLML NVSLALMLAL SLIYPQSVAV NFVAAWAILA TVICVVAGGV GVYATEYVLE
     RYGRELPPES LAVKIVTSLF LQPVPWRRRA AALVVVVATF ISLVAAGWIF TALIYLVVSL
     FFRLIRKACR QRLEGREPCQ G