ANTR4_ARATH
ID ANTR4_ARATH Reviewed; 533 AA.
AC Q66GI9; B3H4C5; Q3EAN9; Q56WL8; Q8W4P5; Q9SD75;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Probable anion transporter 4, chloroplastic;
DE AltName: Full=Phosphate transporter PHT4;3;
DE Flags: Precursor;
GN Name=ANTR4; Synonyms=PHT4;3; OrderedLocusNames=At3g46980;
GN ORFNames=F13I12.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14564522; DOI=10.1007/s00425-003-1121-5;
RA Roth C., Menzel G., Petetot J.M., Rochat-Hacker S., Poirier Y.;
RT "Characterization of a protein of the plastid inner envelope having
RT homology to animal inorganic phosphate, chloride and organic-anion
RT transporters.";
RL Planta 218:406-416(2004).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=18086223; DOI=10.1111/j.1469-8137.2007.02331.x;
RA Guo B., Jin Y., Wussler C., Blancaflor E.B., Motes C.M., Versaw W.K.;
RT "Functional analysis of the Arabidopsis PHT4 family of intracellular
RT phosphate transporters.";
RL New Phytol. 177:889-898(2008).
RN [8]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=19513231; DOI=10.4161/psb.3.10.6666;
RA Guo B., Irigoyen S., Fowler T.B., Versaw W.K.;
RT "Differential expression and phylogenetic analysis suggest specialization
RT of plastid-localized members of the PHT4 phosphate transporter family for
RT photosynthetic and heterotrophic tissues.";
RL Plant Signal. Behav. 3:784-790(2008).
CC -!- FUNCTION: Inorganic phosphate and probable anion transporter.
CC {ECO:0000269|PubMed:18086223}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q66GI9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q66GI9-2; Sequence=VSP_033254, VSP_033255;
CC Name=3;
CC IsoId=Q66GI9-3; Sequence=VSP_041590;
CC -!- TISSUE SPECIFICITY: Expressed in leaf veins and root tips.
CC {ECO:0000269|PubMed:18086223, ECO:0000269|PubMed:19513231}.
CC -!- INDUCTION: Expressed with a circadian rhythm showing a peak during the
CC middle of the day (under long day conditions).
CC {ECO:0000269|PubMed:19513231}.
CC -!- MISCELLANEOUS: [Isoform 3]: Incomplete sequence. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion
CC cotransporter (TC 2.A.1.14) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD94691.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB61944.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL133292; CAB61944.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78226.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78228.1; -; Genomic_DNA.
DR EMBL; AY062442; AAL32520.1; -; mRNA.
DR EMBL; BT015413; AAU05536.1; -; mRNA.
DR EMBL; AK222020; BAD94691.1; ALT_INIT; mRNA.
DR PIR; T45634; T45634.
DR RefSeq; NP_001030824.1; NM_001035747.2. [Q66GI9-3]
DR RefSeq; NP_190282.2; NM_114565.3. [Q66GI9-1]
DR AlphaFoldDB; Q66GI9; -.
DR SMR; Q66GI9; -.
DR BioGRID; 9171; 2.
DR STRING; 3702.AT3G46980.3; -.
DR PaxDb; Q66GI9; -.
DR ProteomicsDB; 240596; -. [Q66GI9-1]
DR EnsemblPlants; AT3G46980.1; AT3G46980.1; AT3G46980. [Q66GI9-1]
DR EnsemblPlants; AT3G46980.3; AT3G46980.3; AT3G46980. [Q66GI9-3]
DR GeneID; 823851; -.
DR Gramene; AT3G46980.1; AT3G46980.1; AT3G46980. [Q66GI9-1]
DR Gramene; AT3G46980.3; AT3G46980.3; AT3G46980. [Q66GI9-3]
DR KEGG; ath:AT3G46980; -.
DR Araport; AT3G46980; -.
DR TAIR; locus:2075586; AT3G46980.
DR eggNOG; KOG2532; Eukaryota.
DR HOGENOM; CLU_001265_5_11_1; -.
DR InParanoid; Q66GI9; -.
DR OMA; YNEQSQM; -.
DR PhylomeDB; Q66GI9; -.
DR PRO; PR:Q66GI9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q66GI9; baseline and differential.
DR Genevisible; Q66GI9; AT.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; IBA:GO_Central.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IDA:TAIR.
DR CDD; cd17380; MFS_SLC17A9_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044777; SLC17A9-like.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Membrane; Plastid; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN ?..533
FT /note="Probable anion transporter 4, chloroplastic"
FT /id="PRO_0000331537"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 438..458
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 474..494
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 502..522
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 462..469
FT /note="EIAPEYSG -> VPKRVIHT (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_033254"
FT VAR_SEQ 470..533
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_033255"
FT VAR_SEQ 533
FT /note="A -> AA (in isoform 3)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_041590"
FT CONFLICT 234
FT /note="P -> T (in Ref. 3; AAL32520)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 533 AA; 58243 MW; 45231CBDC78CB5C7 CRC64;
MCYSLSIQSS IDFHNRNALK IHGDRAILTS NLPTLRRIPF LPERDRRRKL VLCTGRVVNS
LKFTGNTSVD LCGIPRHRLR VSCSDARRTP EETAAELTAQ PNFSEFITSE RVKVVAMLAL
ALALCNADRV VMSVAIVPLS LSRGWSKSFS GIVQSSFLWG YLISPIAGGT LVDRYGGKVV
MAWGVALWSL ATFLTPWAAD SSLWALLAAR AMVGVAEGVA LPCMNNMVAR WFPPTERSRA
VGIAMAGFQL GNVVGLMLSP ILMSQGGIYG PFVIFGLSGF LWLLVWLSAT SSAPDRHPQI
TKSELEYIKQ KKQISTMENK RISTSGIPPF GRLLSKMPTW AVIVANSMHS WGFFVILSWM
PIYFNSVYHV NLKQAAWFSA VPWSMMAFTG YIAGFWSDLL IRRGTSITLT RKIMQSIGFI
GPGIALIGLT TAKQPLVASA WLSLAVGLKS FSHLGFLINL QEIAPEYSGV LHGMCLTAGT
LAAIVGTVGA GFFVELLGSF QGFILLTAIL YLLSALFYNI YATGERVDFD TTA
