ANT_ARATH
ID ANT_ARATH Reviewed; 555 AA.
AC Q38914; Q42462;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=AP2-like ethylene-responsive transcription factor ANT {ECO:0000303|PubMed:8742706, ECO:0000303|PubMed:8742707};
DE AltName: Full=Complementing a protein kinase C mutant protein 1;
DE AltName: Full=Protein AINTEGUMENTA {ECO:0000303|PubMed:8742706, ECO:0000303|PubMed:8742707};
DE AltName: Full=Protein DRAGON;
DE AltName: Full=Protein OVULE MUTANT {ECO:0000303|PubMed:12271029};
GN Name=ANT {ECO:0000303|PubMed:8742706, ECO:0000303|PubMed:8742707};
GN Synonyms=CKC1, DRG, OVM {ECO:0000303|PubMed:12271029};
GN OrderedLocusNames=At4g37750 {ECO:0000312|Araport:AT4G37750};
GN ORFNames=T28I19.30 {ECO:0000312|EMBL:CAB38923.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS
RP OF GLY-382.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=8742706; DOI=10.2307/3870260;
RA Klucher K.M., Chow H., Reiser L., Fischer R.L.;
RT "The AINTEGUMENTA gene of Arabidopsis required for ovule and female
RT gametophyte development is related to the floral homeotic gene APETALA2.";
RL Plant Cell 8:137-153(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Landsberg erecta; TISSUE=Flower;
RX PubMed=8742707; DOI=10.2307/3870261;
RA Elliott R.C., Betzner A.S., Huttner E., Oakes M.P., Tucker W.Q.J.,
RA Gerentes D., Perez P., Smyth D.R.;
RT "AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles
RT in ovule development and floral organ growth.";
RL Plant Cell 8:155-168(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=9001406; DOI=10.1016/s0014-5793(96)01399-3;
RA Vergani P., Morandini P., Soave C.;
RT "Complementation of a yeast delta pkc1 mutant by the Arabidopsis protein
RT ANT.";
RL FEBS Lett. 400:243-246(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Wassilewskija;
RA Fischer R.L., Mizukami Y.;
RT "Methods for altering mass and fertility in plants.";
RL Patent number US6559357, 06-MAY-2003.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [8]
RP FUNCTION, AND REVIEW.
RX PubMed=12271029; DOI=10.2307/3869782;
RA Reiser L., Fischer R.L.;
RT "The ovule and the embryo sac.";
RL Plant Cell 5:1291-1301(1993).
RN [9]
RP FUNCTION.
RX PubMed=9118807; DOI=10.1242/dev.124.7.1367;
RA Schneitz K., Huelskamp M., Kopczak S.D., Pruitt R.E.;
RT "Dissection of sexual organ ontogenesis: a genetic analysis of ovule
RT development in Arabidopsis thaliana.";
RL Development 124:1367-1376(1997).
RN [10]
RP FUNCTION.
RX PubMed=9093862; DOI=10.1093/genetics/145.4.1109;
RA Baker S.C., Robinson-Beers K., Villanueva J.M., Gaiser J.C., Gasser C.S.;
RT "Interactions among genes regulating ovule development in Arabidopsis
RT thaliana.";
RL Genetics 145:1109-1124(1997).
RN [11]
RP DEVELOPMENTAL STAGE.
RX PubMed=9671577; DOI=10.1242/dev.125.16.3027;
RA Long J.A., Barton M.K.;
RT "The development of apical embryonic pattern in Arabidopsis.";
RL Development 125:3027-3035(1998).
RN [12]
RP FUNCTION, AND MUTAGENESIS OF ALA-422.
RX PubMed=10528263;
RX DOI=10.1002/(sici)1520-6408(1999)25:3<224::aid-dvg5>3.0.co;2-y;
RA Krizek B.A.;
RT "Ectopic expression of AINTEGUMENTA in Arabidopsis plants results in
RT increased growth of floral organs.";
RL Dev. Genet. 25:224-236(1999).
RN [13]
RP DEVELOPMENTAL STAGE.
RX PubMed=10656774; DOI=10.1006/dbio.1999.9572;
RA Long J., Barton M.K.;
RT "Initiation of axillary and floral meristems in Arabidopsis.";
RL Dev. Biol. 218:341-353(2000).
RN [14]
RP DNA-BINDING.
RX PubMed=11058102; DOI=10.1093/nar/28.21.4076;
RA Nole-Wilson S., Krizek B.A.;
RT "DNA binding properties of the Arabidopsis floral development protein
RT AINTEGUMENTA.";
RL Nucleic Acids Res. 28:4076-4082(2000).
RN [15]
RP FUNCTION.
RX PubMed=10948255; DOI=10.2307/3871135;
RA Krizek B.A., Prost V., Macias A.;
RT "AINTEGUMENTA promotes petal identity and acts as a negative regulator of
RT AGAMOUS.";
RL Plant Cell 12:1357-1366(2000).
RN [16]
RP FUNCTION.
RX PubMed=11041883; DOI=10.2307/3871199;
RA Liu Z., Franks R.G., Klink V.P.;
RT "Regulation of gynoecium marginal tissue formation by LEUNIG and
RT AINTEGUMENTA.";
RL Plant Cell 12:1879-1891(2000).
RN [17]
RP FUNCTION.
RX PubMed=10639184; DOI=10.1073/pnas.97.2.942;
RA Mizukami Y., Fischer R.L.;
RT "Plant organ size control: AINTEGUMENTA regulates growth and cell numbers
RT during organogenesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:942-947(2000).
RN [18]
RP FUNCTION.
RX PubMed=12183381; DOI=10.1242/dev.129.18.4291;
RA Balasubramanian S., Schneitz K.;
RT "NOZZLE links proximal-distal and adaxial-abaxial pattern formation during
RT ovule development in Arabidopsis thaliana.";
RL Development 129:4291-4300(2002).
RN [19]
RP FUNCTION, AND MUTAGENESIS OF ARG-285; VAL-287; THR-288; HIS-290; TRP-301;
RP ASN-303; GLY-314; VAL-317; TYR-318; LEU-319; TYR-322; ASP-333; LEU-337;
RP TYR-353; GLU-358; HIS-371; PHE-379; SER-380; GLY-382; SER-384; TYR-386;
RP ARG-387; VAL-389; HIS-392; HIS-393; TYR-412; LEU-413; ALA-422; ALA-423;
RP ASP-427; ILE-431; ILE-452; 281-THR--ILE-357; 383-ALA--ARG-451 AND
RP 452-ILE--SER-555.
RX PubMed=12655002; DOI=10.1093/nar/gkg292;
RA Krizek B.A.;
RT "AINTEGUMENTA utilizes a mode of DNA recognition distinct from that used by
RT proteins containing a single AP2 domain.";
RL Nucleic Acids Res. 31:1859-1868(2003).
RN [20]
RP TISSUE SPECIFICITY.
RX PubMed=15988559; DOI=10.1007/s11103-005-0955-6;
RA Nole-Wilson S., Tranby T.L., Krizek B.A.;
RT "AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may
RT specify meristematic or division-competent states.";
RL Plant Mol. Biol. 57:613-628(2005).
RN [21]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
RN [22]
RP INTERACTION WITH ANL2; HDG2; HDG10; GL2; HDG3; HDG8; ATML1 AND PDF2.
RC STRAIN=cv. Columbia;
RX PubMed=25564655; DOI=10.1242/dev.117168;
RA Horstman A., Fukuoka H., Muino J.M., Nitsch L., Guo C., Passarinho P.,
RA Sanchez-Perez G., Immink R., Angenent G., Boutilier K.;
RT "AIL and HDG proteins act antagonistically to control cell proliferation.";
RL Development 142:454-464(2015).
CC -!- FUNCTION: Transcription activator that recognizes and binds to the DNA
CC consensus sequence 5'-CAC[AG]N[AT]TNCCNANG-3'. Required for the
CC initiation and growth of ovules integumenta, and for the development of
CC female gametophyte. Plays a critical role in the development of
CC gynoecium marginal tissues (e.g. stigma, style and septa), and in the
CC fusion of carpels and of medial ridges leading to ovule primordia. Also
CC involved in organs initiation and development, including floral organs.
CC Maintains the meristematic competence of cells and consequently
CC sustains expression of cell cycle regulators during organogenesis, thus
CC controlling the final size of each organ by controlling their cell
CC number. Regulates INO autoinduction and expression pattern. As ANT
CC promotes petal cell identity and mediates down-regulation of AG in
CC flower whorl 2, it functions as a class A homeotic gene.
CC {ECO:0000269|PubMed:10528263, ECO:0000269|PubMed:10639184,
CC ECO:0000269|PubMed:10948255, ECO:0000269|PubMed:11041883,
CC ECO:0000269|PubMed:12183381, ECO:0000269|PubMed:12271029,
CC ECO:0000269|PubMed:12655002, ECO:0000269|PubMed:8742706,
CC ECO:0000269|PubMed:8742707, ECO:0000269|PubMed:9001406,
CC ECO:0000269|PubMed:9093862, ECO:0000269|PubMed:9118807}.
CC -!- SUBUNIT: Interacts with ANL2, HDG2 and HDG10, and possibly with GL2,
CC HDG3, HDG8, ATML1 and PDF2. {ECO:0000269|PubMed:25564655}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in developing flowers. Also
CC present in mature flowers, siliques and seedlings, but not in mature
CC roots, leaves and stems. Expressed in ovules and in vegetative and
CC floral primordia. {ECO:0000269|PubMed:15988559,
CC ECO:0000269|PubMed:8742706, ECO:0000269|PubMed:8742707}.
CC -!- DEVELOPMENTAL STAGE: Expressed in floral primordia, in STM-negative
CC region, then in sepal primordia. As sepal develops, progressively
CC confined to a basal core before disappearing. Present in stamen
CC primordia, then confined to a central region as they become stalked and
CC develop locules. Later reduced to procambial cells as stamen mature.
CC From petal primordia, expressed on the lateral edges of developing
CC petals and finally confined to petal epidermis before disappearing.
CC Present in carpel primordia, then in inner side of carpels especially
CC in the placenta. Strong levels in ovules primordia and young ovules,
CC then localized in integuments initiation zone before being confined to
CC inner integument cells that will differentiate into the endothelium.
CC Expressed in the distal half of the funiculus throughout ovule
CC development and later extends into the chalaza. After fertilization,
CC expression shift to the embryo. First on the apical part at the
CC globular stage, then in cotyledons primordia, and later in cotyledons
CC during the torpedo stage. As cotyledons grow out, expression becomes
CC limited to a plane separating adaxial and abaxial parts. Excluded from
CC the embryonic central region (ECR). In seedlings, found in leaf
CC primordia then in central and lateral actively developing regions of
CC extending leaves. {ECO:0000269|PubMed:10656774,
CC ECO:0000269|PubMed:8742707, ECO:0000269|PubMed:9671577}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. AP2
CC subfamily. {ECO:0000305}.
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DR EMBL; U40256; AAA91040.1; -; mRNA.
DR EMBL; U41339; AAB17364.1; -; mRNA.
DR EMBL; U44028; AAA86281.1; -; mRNA.
DR EMBL; AR316367; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL035709; CAB38923.1; -; Genomic_DNA.
DR EMBL; AL161592; CAB80440.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86834.1; -; Genomic_DNA.
DR EMBL; AY080706; AAL85024.1; -; mRNA.
DR EMBL; AY117207; AAM51282.1; -; mRNA.
DR PIR; S71365; S71365.
DR RefSeq; NP_195489.1; NM_119937.3.
DR AlphaFoldDB; Q38914; -.
DR SMR; Q38914; -.
DR IntAct; Q38914; 1.
DR STRING; 3702.AT4G37750.1; -.
DR PaxDb; Q38914; -.
DR PRIDE; Q38914; -.
DR ProteomicsDB; 240880; -.
DR EnsemblPlants; AT4G37750.1; AT4G37750.1; AT4G37750.
DR GeneID; 829931; -.
DR Gramene; AT4G37750.1; AT4G37750.1; AT4G37750.
DR KEGG; ath:AT4G37750; -.
DR Araport; AT4G37750; -.
DR TAIR; locus:2137559; AT4G37750.
DR eggNOG; ENOG502QQ82; Eukaryota.
DR HOGENOM; CLU_013549_4_0_1; -.
DR InParanoid; Q38914; -.
DR OMA; AETNINI; -.
DR OrthoDB; 455685at2759; -.
DR PhylomeDB; Q38914; -.
DR PRO; PR:Q38914; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q38914; baseline and differential.
DR Genevisible; Q38914; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IMP:TAIR.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0019760; P:glucosinolate metabolic process; IMP:TAIR.
DR GO; GO:0010492; P:maintenance of shoot apical meristem identity; IGI:TAIR.
DR GO; GO:0010601; P:positive regulation of auxin biosynthetic process; IPI:TAIR.
DR GO; GO:0042127; P:regulation of cell population proliferation; IMP:TAIR.
DR CDD; cd00018; AP2; 2.
DR Gene3D; 3.30.730.10; -; 2.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 2.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 2.
DR SUPFAM; SSF54171; SSF54171; 2.
DR PROSITE; PS51032; AP2_ERF; 2.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Differentiation; DNA-binding; Flowering;
KW Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..555
FT /note="AP2-like ethylene-responsive transcription factor
FT ANT"
FT /id="PRO_0000112524"
FT DNA_BIND 283..349
FT /note="AP2/ERF 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT DNA_BIND 385..443
FT /note="AP2/ERF 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 34..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 199..231
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..56
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 281..357
FT /note="Missing: Loss of activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 285
FT /note="R->G: Reduced activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 287
FT /note="V->A: Loss of activation of transcription; when
FT associated with D-303."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 287
FT /note="V->D: Loss of activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 288
FT /note="T->A: Reduced activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 290
FT /note="H->A: Reduced activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 301
FT /note="W->R: Reduced DNA-binding and loss of activation of
FT transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 303
FT /note="N->D: Loss of activation of transcription; when
FT associated with A-287."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 314
FT /note="G->R: Loss of activation of transcription; when
FT associated with C-353."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 317
FT /note="V->D: No effect on DNA-binding but reduced
FT activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 318
FT /note="Y->C: Reduced DNA-binding and loss of activation of
FT transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 319
FT /note="L->P: Loss of activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 319
FT /note="L->Q: Reduced DNA-binding and loss of activation of
FT transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 322
FT /note="Y->F: Loss of activation of transcription; when
FT associated with D-386."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 333
FT /note="D->E: Loss of activation of transcription; when
FT associated with T-452."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 333
FT /note="D->G: Reduced DNA-binding and loss of activation of
FT transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 337
FT /note="L->P: Reduced DNA-binding and loss of activation of
FT transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 353
FT /note="Y->C: Loss of activation of transcription; when
FT associated with R-314."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 358
FT /note="E->G: Loss of activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 371
FT /note="H->P: Loss of activation of transcription; when
FT associated with A-389."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 379
FT /note="F->S: Loss of activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 380
FT /note="S->P: Reduced DNA-binding and reduced activation of
FT transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 382
FT /note="G->D: In ant-2; female-sterile, loss of ovule
FT integuments initiation, impaired female gametophyte
FT development, abnormal floral organs, and loss of activation
FT of transcription."
FT /evidence="ECO:0000269|PubMed:12655002,
FT ECO:0000269|PubMed:8742706"
FT MUTAGEN 383..451
FT /note="Missing: Loss of activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 384
FT /note="S->P: Loss of activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 386
FT /note="Y->D: Loss of activation of transcription; when
FT associated with F-322."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 387
FT /note="R->G: Reduced DNA-binding and loss of activation of
FT transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 389
FT /note="V->A: Loss of activation of transcription; when
FT associated with P-371."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 389
FT /note="V->D: Reduced activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 392
FT /note="H->A: Reduced activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 393
FT /note="H->A: Reduced activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 412
FT /note="Y->C: Reduced activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 413
FT /note="L->Q: Reduced activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 422
FT /note="A->T: In ant-8; abnormal floral organs and ovule
FT development."
FT /evidence="ECO:0000269|PubMed:10528263,
FT ECO:0000269|PubMed:12655002"
FT MUTAGEN 423
FT /note="A->T: Reduced DNA-binding and loss of activation of
FT transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 427
FT /note="D->G: Reduced DNA-binding and loss of activation of
FT transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 431
FT /note="I->S: Reduced DNA-binding and reduced activation of
FT transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 452..555
FT /note="Missing: No effect on activation of transcription."
FT /evidence="ECO:0000269|PubMed:12655002"
FT MUTAGEN 452
FT /note="I->T: Loss of activation of transcription; when
FT associated with E-333."
FT /evidence="ECO:0000269|PubMed:12655002"
FT CONFLICT 45
FT /note="S -> C (in Ref. 3; AAA86281)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 555 AA; 61725 MW; BD275D2F082B519B CRC64;
MKSFCDNDDN NHSNTTNLLG FSLSSNMMKM GGRGGREAIY SSSTSSAATS SSSVPPQLVV
GDNTSNFGVC YGSNPNGGIY SHMSVMPLRS DGSLCLMEAL NRSSHSNHHQ DSSPKVEDFF
GTHHNNTSHK EAMDLSLDSL FYNTTHEPNT TTNFQEFFSF PQTRNHEEET RNYGNDPSLT
HGGSFNVGVY GEFQQSLSLS MSPGSQSSCI TGSHHHQQNQ NQNHQSQNHQ QISEALVETS
VGFETTTMAA AKKKRGQEDV VVVGQKQIVH RKSIDTFGQR TSQYRGVTRH RWTGRYEAHL
WDNSFKKEGH SRKGRQVYLG GYDMEEKAAR AYDLAALKYW GPSTHTNFSA ENYQKEIEDM
KNMTRQEYVA HLRRKSSGFS RGASIYRGVT RHHQHGRWQA RIGRVAGNKD LYLGTFGTQE
EAAEAYDVAA IKFRGTNAVT NFDITRYDVD RIMSSNTLLS GELARRNNNS IVVRNTEDQT
ALNAVVEGGS NKEVSTPERL LSFPAIFALP QVNQKMFGSN MGGNMSPWTS NPNAELKTVA
LTLPQMPVFA AWADS
